Detailed information NTG0184417

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: A9E89_RS04575
• Genome accession: NZ_CP015947
• Coordinates: 1008000..1009064 (+)
• Length: 354 a.a.
• NCBI ID: WP_010946662.1
• Uniprot ID: Q5ZX06
• Organism: Legionella pneumophila strain E2_N
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1003000..1014064

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
A9E89_RS04565 (A9E89_04565)	-	1004037..1006421 (-)	2385	WP_010946660.1	penicillin-binding protein 1A	-
A9E89_RS04570 (A9E89_04570)	ravI	1006573..1007583 (-)	1011	WP_010946661.1	Dot/Icm T4SS effector RavI	-
A9E89_RS04575 (A9E89_04575)	pilM	1008000..1009064 (+)	1065	WP_010946662.1	type IV pilus assembly protein PilM	Machinery gene
A9E89_RS04580 (A9E89_04580)	pilN	1009068..1009616 (+)	549	WP_010946663.1	PilN domain-containing protein	Machinery gene
A9E89_RS04585 (A9E89_04585)	pilO	1009630..1010229 (+)	600	WP_014841277.1	type 4a pilus biogenesis protein PilO	Machinery gene
A9E89_RS04590 (A9E89_04590)	pilP	1010226..1010813 (+)	588	WP_010946665.1	pilus assembly protein PilP	Machinery gene
A9E89_RS04595 (A9E89_04595)	pilQ	1010817..1012913 (+)	2097	WP_010946666.1	type IV pilus secretin PilQ	Machinery gene
A9E89_RS04600 (A9E89_04600)	aroK	1013359..1013886 (+)	528	WP_010946667.1	shikimate kinase AroK	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 38732.71 Da
• Isoelectric Point: 5.0367

>NTDB_id=184417 A9E89_RS04575 WP_010946662.1 1008000..1009064(+) (pilM) [Legionella pneumophila strain E2_N]
MLKLLKPKHRSILGIDISSSAVKILEISGSGEDICVEGYGREEIPASALEGNIIKDVDVVANCIKKLVERLKFTSKNVAL
AVPDSAVISKVVQINEGLTDLEMEELVVIEADKYIPYPIDEINLDFEVLGHSAKSSSLLDVLIVASRAENVNTRVEAAHR
AGLEPKVVDVESYAVERSVQQIAQELPASGQDKIVAIVDIGANYTHLFVLHGMRLIFSREEKFGGMQLVEAIAEHYHMNL
EQAIALKNQGKLPEDYESAVLEPFKDMILLQIKRTLQFFYSTSQHGFVDHILLAGGMARQSGLAALIQEQLGVTTTVANP
LSHMSFGKKVNLDVINSDAPTLMVACGLALRHVE

Nucleotide

• Length: 1065 bp

>NTDB_id=184417 A9E89_RS04575 WP_010946662.1 1008000..1009064(+) (pilM) [Legionella pneumophila strain E2_N]
GTGCTCAAATTGCTTAAACCTAAGCATCGTTCAATTCTTGGCATTGACATCAGTTCTTCCGCAGTGAAAATCCTGGAAAT
ATCAGGTTCCGGAGAGGACATTTGTGTTGAAGGATATGGTCGCGAAGAAATACCTGCCTCTGCCTTAGAAGGAAATATAA
TTAAGGATGTCGATGTAGTAGCGAATTGTATCAAAAAATTAGTAGAAAGATTGAAGTTTACATCTAAAAATGTGGCATTG
GCTGTACCTGATTCGGCTGTTATCAGTAAAGTAGTGCAAATTAATGAAGGGTTAACTGACCTTGAAATGGAAGAGCTTGT
CGTTATTGAGGCGGATAAGTACATTCCTTACCCAATAGATGAAATAAACCTGGATTTTGAAGTGTTAGGCCATTCAGCCA
AGAGCTCATCACTGCTTGATGTTTTAATCGTGGCATCAAGGGCAGAAAACGTTAATACCCGTGTTGAAGCAGCCCATAGA
GCAGGGCTGGAACCCAAAGTTGTTGACGTTGAGTCTTATGCAGTAGAGCGTTCAGTACAACAAATCGCGCAAGAATTACC
AGCAAGTGGGCAAGACAAAATTGTTGCAATAGTAGATATTGGAGCCAATTATACTCACTTATTTGTTTTGCATGGTATGC
GACTAATCTTTTCAAGAGAAGAAAAATTTGGAGGTATGCAACTTGTTGAAGCTATTGCAGAACACTATCATATGAATTTG
GAGCAGGCTATTGCCCTAAAAAATCAGGGTAAACTACCGGAGGACTACGAATCTGCTGTATTAGAACCTTTTAAGGACAT
GATTCTTTTGCAGATCAAACGTACTTTGCAGTTTTTTTATTCAACCAGTCAACATGGATTTGTAGACCATATTTTATTGG
CGGGAGGAATGGCAAGGCAATCAGGATTGGCAGCGCTTATTCAAGAACAGTTAGGAGTGACTACTACAGTGGCAAATCCA
CTATCCCATATGTCTTTTGGTAAAAAAGTGAATTTGGATGTGATTAACAGTGATGCGCCTACACTGATGGTGGCTTGTGG
GTTGGCTTTGAGGCATGTCGAGTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-352)

3D structure

• AlphaFold DB: Q5ZX06

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Legionella pneumophila strain ERS1305867	99.435	100	0.994
comM	Acinetobacter nosocomialis M2	42.817	100	0.429
pilM	Acinetobacter baumannii D1279779	42.535	100	0.427
comM	Acinetobacter baylyi ADP1	41.457	100	0.418