Detailed information NTG0179874

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: A1D33_RS16355
• Genome accession: NZ_CP015443
• Coordinates: 3450492..3451106 (+)
• Length: 204 a.a.
• NCBI ID: WP_015240245.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain CC09
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3445492..3456106

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
A1D33_RS16320 (A1D33_016315)	aroE	3446179..3447021 (+)	843	WP_039254404.1	shikimate dehydrogenase	-
A1D33_RS16325 (A1D33_016320)	yhbY	3447015..3447305 (+)	291	WP_007408257.1	ribosome assembly RNA-binding protein YhbY	-
A1D33_RS16330 (A1D33_016325)	-	3447316..3447885 (+)	570	WP_007408258.1	nicotinate-nucleotide adenylyltransferase	-
A1D33_RS16335 (A1D33_016330)	yqeK	3447875..3448435 (+)	561	WP_014418422.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
A1D33_RS16340 (A1D33_016335)	rsfS	3448453..3448809 (+)	357	WP_007408260.1	ribosome silencing factor	-
A1D33_RS16345 (A1D33_016340)	-	3448806..3449543 (+)	738	WP_015417856.1	class I SAM-dependent DNA methyltransferase	-
A1D33_RS16350 (A1D33_016345)	comER	3449612..3450433 (-)	822	WP_039254409.1	late competence protein ComER	-
A1D33_RS16355 (A1D33_016350)	comEA	3450492..3451106 (+)	615	WP_015240245.1	helix-hairpin-helix domain-containing protein	Machinery gene
A1D33_RS16360 (A1D33_016355)	-	3451173..3451742 (+)	570	WP_003152868.1	ComE operon protein 2	-
A1D33_RS16365 (A1D33_016360)	comEC	3451743..3454094 (+)	2352	WP_062623529.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
A1D33_RS20165	-	3454113..3454247 (-)	135	WP_003152870.1	YqzM family protein	-
A1D33_RS20875	-	3454288..3454439 (+)	152	Protein_3244	hypothetical protein	-
A1D33_RS16370 (A1D33_016365)	holA	3454479..3455520 (+)	1042	Protein_3245	DNA polymerase III subunit delta	-
A1D33_RS16375 (A1D33_016370)	rpsT	3455537..3455803 (-)	267	WP_003152876.1	30S ribosomal protein S20	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22209.32 Da
• Isoelectric Point: 5.5211

>NTDB_id=179874 A1D33_RS16355 WP_015240245.1 3450492..3451106(+) (comEA) [Bacillus velezensis strain CC09]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=179874 A1D33_RS16355 WP_015240245.1 3450492..3451106(+) (comEA) [Bacillus velezensis strain CC09]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGTGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGGGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.854	94.118	0.554
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.545	100	0.368
comEA/celA/cilE	Streptococcus mitis SK321	35.071	100	0.363