Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   CKU_RS07585 Genome accession   NZ_CP014791
Coordinates   1564517..1565203 (-) Length   228 a.a.
NCBI ID   WP_072468915.1    Uniprot ID   A0A5F0HFN7
Organism   Staphylococcus aureus strain MCRF184     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1559517..1570203
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CKU_RS07565 (CKU_1428) rpsT 1560431..1560682 (+) 252 WP_001274017.1 30S ribosomal protein S20 -
  CKU_RS07570 (CKU_1429) holA 1560727..1561701 (-) 975 WP_001282573.1 DNA polymerase III subunit delta -
  CKU_RS07575 (CKU_1430) comEC 1561758..1563959 (-) 2202 WP_001622275.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  CKU_RS07580 (CKU_1431) - 1563964..1564425 (-) 462 WP_000439693.1 ComE operon protein 2 -
  CKU_RS07585 (CKU_1432) comEA 1564517..1565203 (-) 687 WP_072468915.1 ComEA family DNA-binding protein Machinery gene
  CKU_RS07590 (CKU_1433) - 1565243..1565959 (-) 717 WP_000084840.1 class I SAM-dependent DNA methyltransferase -
  CKU_RS07595 (CKU_1434) rsfS 1565962..1566315 (-) 354 WP_001088020.1 ribosome silencing factor -
  CKU_RS07600 (CKU_1435) yqeK 1566316..1566900 (-) 585 WP_001017837.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  CKU_RS07605 (CKU_1436) nadD 1566890..1567459 (-) 570 WP_000725165.1 nicotinate (nicotinamide) nucleotide adenylyltransferase -
  CKU_RS07610 (CKU_1437) yhbY 1567462..1567752 (-) 291 WP_000955234.1 ribosome assembly RNA-binding protein YhbY -
  CKU_RS07615 (CKU_1438) aroE 1567756..1568562 (-) 807 WP_000666750.1 shikimate dehydrogenase -
  CKU_RS07620 (CKU_1439) yqeH 1568576..1569676 (-) 1101 WP_001280146.1 ribosome biogenesis GTPase YqeH -

Sequence

Protein


Download         Length: 228 a.a.        Molecular weight: 25812.41 Da        Isoelectric Point: 9.3305

>NTDB_id=174128 CKU_RS07585 WP_072468915.1 1564517..1565203(-) (comEA) [Staphylococcus aureus strain MCRF184]
MVLLYQFLLRYKDFLTQWKLYIISAVVLIMVLIGFIFWRQDDYTSRDFENKDTALKQSTSENSSLSKLEDVQAKDGDNSK
NKGPVYVDIKGAVKHPNVYKMTSKDRVVDLLDKAQLLDDADVSQINLSEKLTDQKMIFIPHKGQKNVEPQFGENSVHVKN
GNTNNTKVNLNTASVSELMSVPGVGQAKANAIVEYRNQQGAFQEIDDLKKVKGFGSKTFDKLKSYFTT

Nucleotide


Download         Length: 687 bp        

>NTDB_id=174128 CKU_RS07585 WP_072468915.1 1564517..1565203(-) (comEA) [Staphylococcus aureus strain MCRF184]
GTGGTTTTATTGTATCAATTTTTATTACGCTATAAAGATTTTTTAACTCAATGGAAGTTATATATTATAAGTGCTGTTGT
TTTAATTATGGTATTAATTGGTTTTATATTCTGGAGACAAGATGATTATACTTCAAGAGATTTTGAAAATAAAGATACTG
CTCTGAAACAAAGCACTAGTGAAAATAGTAGTTTGTCCAAATTAGAAGATGTCCAGGCCAAAGATGGAGATAATTCCAAA
AATAAAGGTCCTGTATATGTCGATATAAAAGGTGCTGTTAAACACCCTAATGTTTATAAAATGACATCTAAGGATAGAGT
AGTTGATTTACTTGATAAAGCACAATTATTGGATGATGCAGATGTAAGTCAAATTAATTTGTCTGAAAAATTAACAGATC
AAAAAATGATTTTCATTCCTCATAAAGGACAAAAGAATGTTGAACCACAATTTGGAGAAAACAGTGTGCACGTAAAAAAT
GGGAACACAAATAATACTAAAGTAAATTTAAATACGGCATCTGTATCAGAATTGATGTCTGTTCCTGGAGTAGGGCAAGC
TAAAGCTAATGCAATTGTTGAATATCGCAACCAACAAGGTGCATTTCAAGAAATTGACGATTTGAAAAAAGTAAAAGGTT
TTGGAAGTAAAACTTTTGATAAACTGAAATCTTATTTCACGACATAA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(164-227)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5F0HFN7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Staphylococcus aureus MW2

96.476

99.561

0.961
  comEA Staphylococcus aureus N315

96.476

99.561

0.961

Multiple sequence alignment