Detailed information NTG0154396

Bioinformatically predicted

Overview

• Name: comEC/celB
• Type: Machinery gene
• Locus tag: AMD29_RS04250
• Genome accession: NZ_CP012419
• Coordinates: 786925..789162 (+)
• Length: 745 a.a.
• NCBI ID: WP_017647796.1
• Uniprot ID: -
• Organism: Streptococcus agalactiae strain SG-M1
• Function: ssDNA transport into the cell (predicted from homology) DNA binding and uptake

Genomic Context

Location: 781925..794162

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AMD29_RS04225 (AMD29_04220)	-	782519..784105 (+)	1587	WP_000673089.1	DEAD/DEAH box helicase	-
AMD29_RS04230 (AMD29_04225)	-	784290..784556 (-)	267	WP_000598736.1	GIY-YIG nuclease family protein	-
AMD29_RS04235 (AMD29_04230)	-	784549..785313 (-)	765	WP_000567425.1	tRNA1(Val) (adenine(37)-N6)-methyltransferase	-
AMD29_RS04240 (AMD29_04235)	-	785448..786188 (+)	741	WP_000500220.1	lysophospholipid acyltransferase family protein	-
AMD29_RS04245 (AMD29_04240)	-	786288..786941 (+)	654	WP_000461744.1	helix-hairpin-helix domain-containing protein	-
AMD29_RS04250 (AMD29_04245)	comEC/celB	786925..789162 (+)	2238	WP_017647796.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
AMD29_RS04255 (AMD29_04250)	-	789288..790097 (+)	810	WP_000153219.1	Cof-type HAD-IIB family hydrolase	-
AMD29_RS04260 (AMD29_04255)	-	790108..791052 (+)	945	WP_000200830.1	LacI family DNA-binding transcriptional regulator	-
AMD29_RS04265 (AMD29_04260)	-	791111..792103 (-)	993	WP_000800998.1	alpha/beta hydrolase fold domain-containing protein	-
AMD29_RS04270 (AMD29_04265)	-	792279..793007 (+)	729	WP_000468966.1	methyltransferase domain-containing protein	-
AMD29_RS04275 (AMD29_04270)	holA	793061..794098 (+)	1038	WP_000560292.1	DNA polymerase III subunit delta	-

Sequence

Protein

• Length: 745 a.a.
• Molecular weight: 85380.33 Da
• Isoelectric Point: 9.8236

>NTDB_id=154396 AMD29_RS04250 WP_017647796.1 786925..789162(+) (comEC/celB) [Streptococcus agalactiae strain SG-M1]
MLQLTKYFPLKPIYLALLVFQIYLLVFSWTMLGCAFLLFSFIFLIYQYDRETIFKTIAIVIFFLFYFLWQNHNMNVQYQR
VPNHISQIKVRIDTISINGDVLSFQADASGNTCQAFYTLKNKSEKDYFQNLDNNIMIIADIKLEEAEERRHFNGFDYRQY
LKRHGIYRIAKVTKIKQIRLFQHRSFFALMSKWRRSAIVISQTFPNPMRHYMSGLLFGYLDKTFDDMSDLYSSLGIIHLF
ALSGMQVGFFLGIFRYICLRIGLRLDHVWLLQIPFSLIYAGLTGFSISVVRALIQSLLSHSGVKKDENFALCLLICLISL
PHSLLTTGGVLSFAYAFILTMTSFDHFSSIKKVAIESLTVSVGILPILTYYFSGFQPISIILTALLSFAFDIIFLPLLTV
IFVLSPIVKLSCINSLFEILEVLLKWTGQLFPRPLIFGKPSLFLLIVMIIILGLLYDYYHSKCFRYCSLLIIFTLFFITK
NPITNEVAILDVGQGDSILVRDWLGKTILIDTGGRVRFEQPEEWKQKVNQSNAKRTLIPYLKSRGISKIDDLVITHTDTD
HMGDMEVISKHFKVARLITSSGSLTNSQYVKHLSKIGVAVKSIEAGDKLAVMGSYLQVLYPWHKGDGKNNDSIVLYGHLL
GKGFLFTGDLEEEGEKQLLEAYPNLSVDILKAGHHGSKGSSSLSFLKKLSPSVVLVSAGKNNRYQHPHQETLQRFQKIKS
KIFRTDQSGTIRLTGWWKWHIQTVR

Nucleotide

• Length: 2238 bp

>NTDB_id=154396 AMD29_RS04250 WP_017647796.1 786925..789162(+) (comEC/celB) [Streptococcus agalactiae strain SG-M1]
ATGTTACAATTGACTAAGTATTTTCCTCTAAAACCTATTTATTTAGCATTGTTGGTCTTCCAAATTTACTTACTAGTGTT
TTCTTGGACAATGCTTGGTTGTGCCTTTCTTTTATTTTCTTTTATTTTTCTGATTTATCAATATGATCGTGAAACTATTT
TTAAAACAATAGCAATAGTAATTTTTTTCTTATTTTATTTTTTATGGCAAAATCACAATATGAATGTCCAATATCAAAGA
GTACCGAATCATATTAGCCAGATTAAAGTGCGTATTGATACTATTTCTATCAATGGTGATGTTTTATCATTCCAGGCAGA
TGCTTCAGGTAACACTTGTCAAGCTTTTTACACATTAAAAAATAAAAGTGAGAAAGATTATTTTCAAAATCTTGATAATA
ATATAATGATAATTGCAGATATCAAACTTGAAGAAGCAGAGGAGAGAAGGCATTTTAATGGCTTTGATTATCGTCAGTAT
TTAAAAAGACATGGAATTTATCGTATCGCCAAAGTGACAAAGATAAAACAGATACGCTTATTTCAACATAGGTCTTTCTT
TGCTCTTATGTCTAAGTGGCGTAGAAGTGCAATTGTTATTAGTCAAACTTTTCCAAATCCTATGCGTCACTATATGTCAG
GGCTTTTGTTTGGATATCTAGATAAGACCTTTGATGACATGTCCGATTTATATAGTAGTCTAGGTATTATACATTTATTT
GCTTTGTCAGGTATGCAAGTAGGTTTTTTTCTCGGTATTTTTCGTTATATCTGTCTACGTATTGGCTTACGTCTAGACCA
TGTTTGGTTACTTCAAATACCATTCTCGCTAATTTATGCTGGTTTAACAGGCTTTAGTATCTCAGTCGTTAGGGCACTTA
TTCAATCTTTATTATCACATAGCGGTGTCAAGAAAGATGAGAACTTTGCTCTCTGCTTGTTAATTTGTCTTATCTCCCTC
CCCCACTCACTTTTGACTACGGGAGGAGTTCTTAGCTTTGCTTATGCTTTTATACTTACGATGACCTCCTTTGATCATTT
TTCGAGTATAAAAAAAGTAGCTATCGAATCTTTGACAGTCTCTGTAGGAATTCTTCCCATACTAACCTACTATTTTTCGG
GTTTTCAACCAATATCAATTATATTAACAGCACTTTTATCTTTTGCATTTGATATTATATTTTTGCCTTTATTAACTGTT
ATATTTGTCTTATCGCCTATCGTTAAATTAAGTTGTATTAATAGTTTGTTTGAAATCCTAGAAGTGTTATTAAAATGGAC
TGGGCAACTGTTTCCAAGGCCACTTATTTTTGGAAAGCCCAGCCTTTTTCTTTTAATAGTCATGATTATAATTTTGGGAT
TACTTTATGATTATTATCATTCTAAATGTTTTCGTTATTGCTCCCTTCTTATTATCTTTACCTTGTTTTTTATCACTAAG
AATCCAATTACTAACGAGGTTGCGATTTTAGATGTTGGACAGGGAGATAGTATTTTAGTGAGGGATTGGTTAGGAAAAAC
AATTTTAATTGATACTGGGGGAAGGGTGAGATTTGAACAGCCTGAAGAATGGAAACAAAAAGTAAATCAGTCTAATGCTA
AGAGAACGCTCATTCCTTACTTGAAAAGCAGAGGTATTAGCAAGATAGATGATTTAGTGATAACTCATACCGATACAGAT
CATATGGGGGATATGGAAGTTATCTCAAAGCATTTTAAAGTTGCACGTTTGATTACAAGTTCAGGTTCTTTAACGAATTC
GCAGTACGTTAAGCATTTATCAAAGATAGGTGTAGCGGTAAAATCTATAGAAGCCGGTGATAAACTTGCTGTCATGGGAA
GTTATTTACAAGTACTTTACCCATGGCACAAGGGTGATGGAAAAAATAATGATTCAATTGTTTTATATGGACATTTATTA
GGAAAAGGCTTCTTATTTACCGGTGATTTGGAGGAAGAGGGAGAAAAGCAGTTATTAGAAGCTTATCCTAATTTATCAGT
AGATATCCTTAAAGCAGGACATCATGGTTCTAAGGGCTCATCAAGTCTATCCTTTCTGAAAAAGTTGTCTCCTAGTGTGG
TTCTAGTTTCAGCTGGTAAAAATAATCGTTACCAGCATCCTCATCAAGAGACTTTACAAAGGTTCCAAAAGATTAAAAGC
AAGATTTTCCGAACGGATCAATCAGGTACAATTAGGCTAACAGGATGGTGGAAGTGGCATATTCAGACAGTTCGTTGA

Domains

Predicted by InterproScan.

• Competence protein (Competence) (215-458)

• Domain of unknown function (DUF4131) (DUF4131) (27-177)

• Metallo-beta-lactamase superfamily (Lactamase_B) (491-576)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEC/celB	Streptococcus mitis NCTC 12261	48.4	100	0.487
comEC/celB	Streptococcus mitis SK321	48.529	100	0.487
comEC/celB	Streptococcus pneumoniae TIGR4	47.059	100	0.472
comEC/celB	Streptococcus pneumoniae Rx1	46.586	100	0.467
comEC/celB	Streptococcus pneumoniae D39	46.586	100	0.467
comEC/celB	Streptococcus pneumoniae R6	46.586	100	0.467
comEC	Lactococcus lactis subsp. cremoris KW2	43.203	99.732	0.431