Detailed information    

insolico Bioinformatically predicted

Overview


Name   ssb   Type   Machinery gene
Locus tag   BF28_RS20255 Genome accession   NZ_CP009968
Coordinates   3381188..3381526 (-) Length   112 a.a.
NCBI ID   WP_000982031.1    Uniprot ID   A0A6L8PNC7
Organism   Bacillus cereus E33L     
Function   ssDNA binding (predicted from homology)   
DNA processing

Genomic Context


Location: 3376188..3386526
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BF28_RS20225 (BF28_3505) - 3376220..3376996 (-) 777 WP_000637466.1 class I SAM-dependent methyltransferase -
  BF28_RS20230 (BF28_3506) - 3377046..3377480 (-) 435 WP_000063712.1 BrxA/BrxB family bacilliredoxin -
  BF28_RS20235 (BF28_3507) - 3377564..3377947 (-) 384 WP_000634865.1 thiol-disulfide oxidoreductase DCC family protein -
  BF28_RS20240 (BF28_3508) - 3377951..3379078 (-) 1128 WP_000692577.1 conserved virulence factor C family protein -
  BF28_RS20245 (BF28_3509) - 3379337..3380470 (+) 1134 WP_001066854.1 FAD-dependent oxidoreductase -
  BF28_RS20250 (BF28_3510) - 3380572..3381162 (+) 591 WP_000943174.1 TetR/AcrR family transcriptional regulator -
  BF28_RS20255 (BF28_3511) ssb 3381188..3381526 (-) 339 WP_000982031.1 single-stranded DNA-binding protein Machinery gene
  BF28_RS20260 (BF28_3512) - 3381685..3381939 (-) 255 WP_000975148.1 DUF4318 domain-containing protein -
  BF28_RS20265 (BF28_3513) - 3382045..3382776 (-) 732 WP_001260645.1 Bax inhibitor-1/YccA family membrane protein -
  BF28_RS20270 (BF28_3514) - 3382852..3384747 (-) 1896 WP_000783199.1 ABC-F family ATP-binding cassette domain-containing protein -
  BF28_RS20275 (BF28_3515) - 3384744..3385391 (-) 648 WP_000165938.1 HD domain-containing protein -
  BF28_RS20280 (BF28_3516) - 3385501..3386304 (+) 804 WP_000499702.1 hypothetical protein -

Sequence


Protein


Download         Length: 112 a.a.        Molecular weight: 12893.74 Da        Isoelectric Point: 9.0926

>NTDB_id=133236 BF28_RS20255 WP_000982031.1 3381188..3381526(-) (ssb) [Bacillus cereus E33L]
MMNRVVLIGRLTKEPELYYTKQGVAYARVCVAVNRGFRNSLGEQQVDFINCVVWRKSAENVTEYCTKGSLVGITGRIHTR
NYEDDQGKRIYITEVVIESITFLERRREGASQ

Nucleotide


Download         Length: 339 bp        

>NTDB_id=133236 BF28_RS20255 WP_000982031.1 3381188..3381526(-) (ssb) [Bacillus cereus E33L]
ATGATGAATCGAGTTGTATTAATCGGTAGATTGACAAAGGAGCCAGAATTATACTACACAAAGCAAGGCGTCGCGTATGC
ACGAGTATGTGTTGCGGTGAATAGAGGATTTCGAAATAGTTTAGGTGAACAACAAGTAGATTTTATTAATTGTGTCGTTT
GGCGAAAATCGGCTGAGAATGTAACTGAATATTGTACGAAAGGGTCTCTTGTTGGAATTACCGGACGTATTCATACGAGG
AATTACGAGGATGATCAAGGAAAGAGAATATATATAACGGAAGTCGTGATTGAGAGCATTACATTTTTGGAGAGAAGGCG
CGAAGGTGCATCGCAATAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure
  AlphaFold DB A0A6L8PNC7

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssb Latilactobacillus sakei subsp. sakei 23K

52.941

100

0.562

  ssbA Bacillus subtilis subsp. subtilis str. 168

58.491

94.643

0.554

  ssbB Bacillus subtilis subsp. subtilis str. 168

51.327

100

0.518

  ssbB Streptococcus sobrinus strain NIDR 6715-7

45.455

98.214

0.446

  ssbB Lactococcus lactis subsp. cremoris KW2

47.619

93.75

0.446

  ssbA Streptococcus mutans UA159

43.636

98.214

0.429

  ssbB/cilA Streptococcus pneumoniae TIGR4

42.727

98.214

0.42

  ssbB/cilA Streptococcus mitis NCTC 12261

42.727

98.214

0.42

  ssbB/cilA Streptococcus pneumoniae Rx1

41.818

98.214

0.411

  ssbB/cilA Streptococcus pneumoniae D39

41.818

98.214

0.411

  ssbB/cilA Streptococcus pneumoniae R6

41.818

98.214

0.411

  ssbB/cilA Streptococcus mitis SK321

41.818

98.214

0.411


Multiple sequence alignment