Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACEE_RS05215 Genome accession   NZ_CP007715
Coordinates   1147232..1148614 (-) Length   460 a.a.
NCBI ID   WP_039197144.1    Uniprot ID   -
Organism   Actinobacillus equuli subsp. equuli strain 19392     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1142232..1153614
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACEE_RS05185 (ACEE_05310) lptE 1142838..1143332 (+) 495 WP_005597385.1 LPS assembly lipoprotein LptE -
  ACEE_RS05190 (ACEE_05315) holA 1143440..1144465 (+) 1026 WP_039197140.1 DNA polymerase III subunit delta -
  ACEE_RS05195 (ACEE_05320) yacG 1144542..1144730 (-) 189 WP_005623139.1 DNA gyrase inhibitor YacG -
  ACEE_RS05200 (ACEE_05325) coaE 1144712..1145350 (-) 639 WP_039197141.1 dephospho-CoA kinase -
  ACEE_RS05205 (ACEE_05330) - 1145372..1146046 (-) 675 WP_039197142.1 fimbrial protein -
  ACEE_RS05210 (ACEE_05335) pilC 1146046..1147239 (-) 1194 WP_039197143.1 type II secretion system F family protein Machinery gene
  ACEE_RS05215 (ACEE_05340) pilB 1147232..1148614 (-) 1383 WP_039197144.1 GspE/PulE family protein Machinery gene
  ACEE_RS05220 (ACEE_05345) pilA 1148635..1149072 (-) 438 WP_039197145.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  ACEE_RS05225 (ACEE_05350) radA 1149253..1150632 (+) 1380 WP_039197146.1 DNA repair protein RadA Machinery gene
  ACEE_RS05230 (ACEE_05355) pepB 1150698..1151981 (+) 1284 WP_039199020.1 aminopeptidase PepB -
  ACEE_RS05235 (ACEE_05360) - 1152109..1153044 (+) 936 WP_039197147.1 SIS domain-containing protein -
  ACEE_RS05240 (ACEE_05365) greB 1153054..1153542 (+) 489 WP_005611555.1 transcription elongation factor GreB -

Sequence

Protein


Download         Length: 460 a.a.        Molecular weight: 52340.96 Da        Isoelectric Point: 5.4931

>NTDB_id=122845 ACEE_RS05215 WP_039197144.1 1147232..1148614(-) (pilB) [Actinobacillus equuli subsp. equuli strain 19392]
MQYSVCDMNSQQVFDISESLWQKNCDEKHILLRYLAIPIQENDDTLWLAIDDMNNLSACEIFAFIAHKTIEPVLISSDEL
KYLLNQLSPEQQSLYEETELNYQSPDNLELLNQNDPIIQILDNLFKFCLKQNASDIHFEPQKEKLLIRLRIDGVLHIYKS
LSFSLANRIITRLKLLAKLDISETRLPQDGQFNFKTVTAETLDFRVSSLPTSFGEKIVLRLQKNKPTHFDFLELGFSPEQ
KQLLLAALHQPQGLILVTGPTGSGKSITLYSALNYLNNSERHILTADPIEIEIEGIIQTQVNRAIQLDFSQLLRTFLRQD
PDIIMLGEIRDQESAEIALRAAQTGHLVLSTLHTNDAPSAIERLLQLGIKEYEIKNSLQLIIAQRLLRKRCPQCGGKGCD
NCYHGYKGRTGIYQLLTKQGKFFEKSTAYLDYDSLRASAEQKLAANITTVDEVKRVLGDG

Nucleotide


Download         Length: 1383 bp        

>NTDB_id=122845 ACEE_RS05215 WP_039197144.1 1147232..1148614(-) (pilB) [Actinobacillus equuli subsp. equuli strain 19392]
ATGCAGTATTCAGTTTGTGATATGAATAGTCAGCAAGTATTTGATATCTCTGAATCCTTGTGGCAGAAAAATTGTGACGA
AAAACATATTTTGTTGCGTTATTTAGCCATTCCGATTCAAGAAAATGACGATACGCTATGGCTTGCAATTGATGATATGA
ATAATTTAAGTGCTTGTGAAATTTTTGCATTTATTGCGCATAAAACAATTGAACCTGTTTTGATTTCCTCCGATGAATTG
AAGTACTTACTTAATCAACTTTCGCCGGAACAACAATCTTTATATGAAGAAACCGAGTTAAATTATCAATCGCCAGATAA
TTTAGAACTACTCAATCAAAATGATCCTATTATTCAGATTTTAGATAATTTATTTAAATTTTGCTTAAAGCAAAATGCTT
CGGATATTCATTTTGAGCCACAAAAAGAGAAGTTACTGATTAGATTACGTATTGATGGGGTATTGCATATCTATAAAAGC
TTATCGTTTAGTTTAGCCAATCGTATTATCACAAGGCTTAAACTCTTAGCTAAATTGGATATTAGTGAGACACGCTTACC
ACAAGACGGGCAGTTTAATTTTAAGACGGTAACAGCTGAGACATTAGATTTTCGAGTGTCCAGTTTACCGACTAGCTTCG
GTGAGAAAATAGTCTTGCGCTTGCAAAAGAATAAGCCTACCCATTTTGATTTTTTGGAATTAGGTTTTTCGCCGGAACAA
AAACAATTACTACTGGCAGCACTACATCAGCCACAAGGGCTAATTCTGGTGACAGGACCTACGGGTAGTGGCAAAAGTAT
TACTTTATATAGTGCGTTAAATTATTTAAATAATAGCGAACGGCATATTCTGACGGCAGACCCGATTGAAATTGAGATAG
AAGGGATTATCCAAACTCAAGTTAATCGTGCTATTCAATTGGATTTTAGTCAATTACTCAGAACATTTTTACGTCAGGAT
CCGGACATTATTATGTTAGGTGAAATCCGTGATCAGGAAAGTGCTGAAATCGCATTAAGAGCAGCGCAAACAGGACATCT
TGTACTTTCTACTTTACATACGAATGATGCGCCGTCTGCGATTGAGCGTTTACTACAATTAGGGATTAAAGAGTATGAGA
TTAAGAATTCACTGCAACTGATTATCGCACAGCGTTTATTACGTAAACGTTGTCCGCAATGTGGCGGAAAAGGATGTGAT
AACTGTTATCACGGTTATAAAGGACGAACGGGAATTTATCAGTTATTGACGAAGCAAGGTAAATTTTTTGAAAAATCAAC
CGCTTATTTGGATTATGACAGCTTACGAGCCAGTGCCGAACAAAAGTTAGCGGCTAACATAACAACAGTAGACGAAGTAA
AGCGGGTGTTAGGTGATGGTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(129-389)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(29-93)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Glaesserella parasuis strain SC1401

72.707

99.565

0.724
  pilB Haemophilus influenzae Rd KW20

56.984

98.043

0.559
  pilB Haemophilus influenzae 86-028NP

56.319

98.043

0.552
  pilB Legionella pneumophila strain ERS1305867

39.837

100

0.426
  pilB Vibrio parahaemolyticus RIMD 2210633

40.748

100

0.426
  pilB Vibrio campbellii strain DS40M4

40

100

0.422
  pilB Vibrio cholerae strain A1552

38.554

100

0.417
  pilB Acinetobacter baumannii D1279779

38.306

100

0.413
  pilB Acinetobacter baylyi ADP1

37.575

100

0.411

Multiple sequence alignment