Detailed information NTG0121108

Bioinformatically predicted

Overview

• Name: comEC/celB
• Type: Machinery gene
• Locus tag: DK42_RS04540
• Genome accession: NZ_CP007571
• Coordinates: 869836..872073 (+)
• Length: 745 a.a.
• NCBI ID: WP_000939910.1
• Uniprot ID: -
• Organism: Streptococcus agalactiae strain GBS2-NM
• Function: ssDNA transport into the cell (predicted from homology) DNA binding and uptake

Genomic Context

Location: 864836..877073

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
DK42_RS04515 (DK42_04630)	-	865431..867017 (+)	1587	WP_000673086.1	DEAD/DEAH box helicase	-
DK42_RS04520 (DK42_04635)	-	867202..867468 (-)	267	WP_000598736.1	GIY-YIG nuclease family protein	-
DK42_RS04525 (DK42_04640)	-	867461..868225 (-)	765	WP_000567428.1	tRNA1(Val) (adenine(37)-N6)-methyltransferase	-
DK42_RS04530 (DK42_04645)	-	868360..869100 (+)	741	WP_000500220.1	1-acyl-sn-glycerol-3-phosphate acyltransferase	-
DK42_RS04535 (DK42_04650)	-	869199..869852 (+)	654	WP_000461748.1	helix-hairpin-helix domain-containing protein	-
DK42_RS04540 (DK42_04655)	comEC/celB	869836..872073 (+)	2238	WP_000939910.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
DK42_RS04545 (DK42_04660)	-	872199..873008 (+)	810	WP_000153220.1	Cof-type HAD-IIB family hydrolase	-
DK42_RS04550 (DK42_04665)	-	873019..873963 (+)	945	WP_000200832.1	LacI family DNA-binding transcriptional regulator	-
DK42_RS04555 (DK42_04670)	-	874022..875014 (-)	993	WP_000800992.1	alpha/beta hydrolase	-
DK42_RS04560 (DK42_04675)	-	875190..875918 (+)	729	WP_000468970.1	methyltransferase domain-containing protein	-
DK42_RS04565 (DK42_04680)	holA	875972..877009 (+)	1038	WP_000560292.1	DNA polymerase III subunit delta	-

Sequence

Protein

• Length: 745 a.a.
• Molecular weight: 85597.41 Da
• Isoelectric Point: 9.6952

>NTDB_id=121108 DK42_RS04540 WP_000939910.1 869836..872073(+) (comEC/celB) [Streptococcus agalactiae strain GBS2-NM]
MLQLTKYFPLKPIYLALLVFQIYLLVFSWTMLGCAFLLFTFIFLIYQYDRETIFKTIAIVIFFLFYFLWQNHNMNVQYQR
VPNHISQIEVRIDTISINGDVLSFQADASGNTYQAFYTLKNKSEKDYFQNLDNNIMIIADIKLEEAEERRHFNGFDYRQY
LKRHGIYRIAKVTKIKQIRLFQHRSFFDLMSKWRRSAIVISQTFPNPMRHYMSGLLFGYLDKTFDDMSDLYSSLGIIHLF
ALSGMQVGFFLGIFRYICLRIGLRLDHVWLLQIPFSLIYAGLTGFSISVVRALIQSLLSHSGVKKDENFAFCLLICLISL
PHSLLTTGGVLSFAYAFILTMTSFDHFSSIKKLAIESLTVSVGILPILTYYFSGFQPISIILTALLSFAFDIIFLPLLTV
IFVLSPIVKLSCINSLFEILEVLLKWTGQLFPRPLIFGKPSLFLLIVMIIILGLLYDYYHSKCFRYCSLLIIFTLFFITK
NPITNEVAILDVGQGDSILVRDWLGKTILIDTGGRLRFEQPEEWKQKVNQSNAERTLIPYLKSRGISKIDDLVITHTDTD
HMGDMEVISKHFKVARLITSSGSLTNSQYVKRLSKIGVAVKSIEAGDKLSVMGSYLQVLYPWHKGDGKNNDSIVLYGHLL
GKGFLFTGDLEEEGEKQLLEAYPNLSVDILKAGHHGSKGSSSLSFLKKLSPSVVLVSAGKNNRYQHPHQETLQRFQKIKS
KIFRTDQSGTIRLTGWWKWHIQTVR

Nucleotide

• Length: 2238 bp

>NTDB_id=121108 DK42_RS04540 WP_000939910.1 869836..872073(+) (comEC/celB) [Streptococcus agalactiae strain GBS2-NM]
ATGTTACAATTGACTAAGTATTTTCCTCTAAAACCTATTTATTTAGCATTGTTGGTCTTCCAAATTTACTTACTAGTGTT
TTCTTGGACAATGCTTGGTTGTGCCTTTCTTTTATTTACTTTTATTTTTCTGATTTATCAATATGATCGTGAAACTATTT
TTAAAACAATAGCAATAGTAATTTTTTTCTTATTTTATTTTTTATGGCAAAATCACAATATGAATGTCCAATATCAAAGA
GTACCGAATCATATTAGCCAGATTGAAGTGCGTATTGATACTATTTCTATCAATGGTGATGTTTTATCATTCCAGGCAGA
TGCTTCAGGTAACACTTATCAAGCTTTTTACACATTAAAAAATAAAAGTGAGAAAGATTATTTTCAAAATCTTGATAATA
ATATAATGATAATTGCAGATATCAAACTTGAAGAAGCAGAGGAGAGAAGGCATTTTAATGGCTTTGATTATCGTCAGTAT
TTAAAAAGACATGGAATTTATCGTATCGCCAAAGTGACAAAGATAAAACAGATACGCTTATTTCAACATAGGTCTTTCTT
TGATCTTATGTCTAAGTGGCGTAGAAGTGCAATTGTTATTAGTCAAACTTTTCCAAATCCTATGCGTCACTATATGTCAG
GGCTTTTGTTTGGATATCTAGATAAGACCTTTGATGACATGTCCGATTTATATAGTAGTCTAGGTATTATACATTTATTT
GCTTTGTCAGGTATGCAAGTAGGTTTTTTTCTCGGTATTTTTCGTTATATCTGTCTACGTATTGGCTTACGCCTAGACCA
TGTTTGGTTACTTCAAATACCATTCTCGCTAATTTATGCTGGTTTAACAGGCTTTAGTATCTCAGTCGTTAGGGCACTTA
TTCAATCTTTATTATCACATAGCGGTGTCAAGAAAGATGAGAACTTTGCTTTCTGCTTGTTAATTTGTCTTATCTCTCTC
CCCCACTCACTTTTGACTACGGGAGGAGTTCTTAGCTTTGCTTATGCTTTTATACTTACGATGACCTCCTTTGATCATTT
TTCGAGTATAAAAAAACTAGCTATCGAATCTTTGACAGTCTCTGTAGGAATTCTTCCCATACTAACCTACTATTTTTCAG
GTTTTCAACCAATATCAATTATATTAACAGCACTTTTATCTTTTGCATTTGATATTATATTTTTGCCTTTATTAACTGTT
ATATTTGTCTTATCGCCTATCGTTAAATTAAGTTGTATTAATAGTTTGTTTGAAATCCTAGAAGTGTTATTAAAATGGAC
TGGGCAACTGTTTCCAAGGCCACTTATTTTTGGAAAGCCCAGCCTTTTTCTTTTAATAGTCATGATTATAATTTTGGGAT
TACTTTATGATTATTATCATTCTAAATGTTTTCGTTATTGCTCCCTTCTTATTATCTTTACCTTGTTTTTTATCACTAAG
AATCCAATTACTAACGAGGTCGCGATTTTAGATGTTGGACAAGGAGATAGTATTTTAGTGAGGGATTGGTTAGGAAAAAC
AATTTTAATTGATACTGGGGGAAGGTTGAGATTTGAACAGCCTGAAGAATGGAAACAAAAAGTAAATCAGTCTAATGCTG
AGAGAACGCTCATTCCTTACTTGAAAAGCAGAGGTATTAGCAAGATAGATGATTTAGTGATAACTCATACCGATACAGAT
CATATGGGGGATATGGAAGTTATCTCAAAGCATTTTAAAGTTGCACGTTTGATTACAAGTTCAGGTTCTTTAACGAATTC
GCAGTACGTTAAGCGTTTATCAAAGATAGGAGTAGCTGTAAAATCTATAGAAGCTGGTGATAAACTTTCTGTCATGGGAA
GTTATTTACAAGTACTTTACCCATGGCACAAGGGTGATGGAAAAAATAATGATTCAATTGTTTTATATGGACATTTATTA
GGAAAAGGTTTCTTATTTACCGGTGATTTGGAGGAAGAGGGAGAAAAGCAGTTATTAGAAGCTTATCCTAATTTATCAGT
AGATATCCTTAAAGCAGGACATCATGGTTCTAAGGGCTCATCAAGTCTATCCTTTCTGAAAAAGTTGTCTCCTAGTGTGG
TGCTAGTGTCAGCTGGTAAAAATAATCGTTACCAGCATCCTCATCAAGAGACTTTACAAAGGTTCCAAAAGATTAAAAGC
AAGATTTTCCGAACGGATCAATCAGGTACAATTAGGCTAACAGGATGGTGGAAGTGGCATATTCAGACAGTTCGTTGA

Domains

Predicted by InterproScan.

• Domain of unknown function (DUF4131) (DUF4131) (28-177)

• Competence protein (Competence) (215-458)

• Metallo-beta-lactamase superfamily (Lactamase_B) (491-576)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEC/celB	Streptococcus mitis NCTC 12261	48.267	100	0.486
comEC/celB	Streptococcus mitis SK321	48.262	100	0.485
comEC/celB	Streptococcus pneumoniae TIGR4	46.791	100	0.47
comEC/celB	Streptococcus pneumoniae Rx1	46.319	100	0.464
comEC/celB	Streptococcus pneumoniae D39	46.319	100	0.464
comEC/celB	Streptococcus pneumoniae R6	46.319	100	0.464
comEC	Lactococcus lactis subsp. cremoris KW2	44.28	99.732	0.442