Detailed information NTG1156530

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: KJP78_RS14060
• Genome accession: NZ_OX419553
• Coordinates: 2665975..2666592 (-)
• Length: 205 a.a.
• NCBI ID: WP_004398514.1
• Uniprot ID: A0AAE2SLR2
• Organism: Bacillus subtilis isolate NRS6183
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2660975..2671592

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
KJP78_RS14030 (NRS6183_13965)	rpsT	2661277..2661543 (+)	267	WP_003229989.1	30S ribosomal protein S20	-
KJP78_RS14035 (NRS6183_13970)	holA	2661558..2662601 (-)	1044	WP_003229987.1	DNA polymerase III subunit delta	-
KJP78_RS14040	-	2662641..2662790 (-)	150	WP_003229985.1	hypothetical protein	-
KJP78_RS14045 (NRS6183_13975)	yqzM	2662831..2662965 (+)	135	WP_003229983.1	YqzM family protein	-
KJP78_RS14050 (NRS6183_13980)	comEC	2663005..2665335 (-)	2331	WP_198878618.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
KJP78_RS14055 (NRS6183_13985)	comEB	2665339..2665908 (-)	570	WP_003229978.1	ComE operon protein 2	-
KJP78_RS14060 (NRS6183_13990)	comEA	2665975..2666592 (-)	618	WP_004398514.1	competence protein ComEA	Machinery gene
KJP78_RS14065 (NRS6183_13995)	comER	2666676..2667497 (+)	822	WP_004398597.1	late competence protein ComER	-
KJP78_RS14070 (NRS6183_14000)	yqeM	2667563..2668306 (-)	744	WP_003229973.1	class I SAM-dependent methyltransferase	-
KJP78_RS14075 (NRS6183_14005)	rsfS	2668303..2668659 (-)	357	WP_003229971.1	ribosome silencing factor	-
KJP78_RS14080 (NRS6183_14010)	yqeK	2668677..2669237 (-)	561	WP_004399059.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
KJP78_RS14085 (NRS6183_14015)	nadD	2669227..2669796 (-)	570	WP_004398676.1	nicotinate-nucleotide adenylyltransferase	-
KJP78_RS14090 (NRS6183_14020)	yhbY	2669808..2670098 (-)	291	WP_003226133.1	ribosome assembly RNA-binding protein YhbY	-
KJP78_RS14095 (NRS6183_14025)	aroE	2670092..2670934 (-)	843	WP_003229967.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 205 a.a.
• Molecular weight: 21769.45 Da
• Isoelectric Point: 4.7220

>NTDB_id=1156530 KJP78_RS14060 WP_004398514.1 2665975..2666592(-) (comEA) [Bacillus subtilis isolate NRS6183]
MNWLNQHKKAIILAASAAVFTAIMIFLATGKNKEPVKQAVPTETENTVVKQEANNDESNETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTSEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide

• Length: 618 bp

>NTDB_id=1156530 KJP78_RS14060 WP_004398514.1 2665975..2666592(-) (comEA) [Bacillus subtilis isolate NRS6183]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTATTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGACAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAACGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGCGTTTATGAAATGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGCGGAGGGTCTG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAGGAAAACGGTCGTTTCCAAACAATTGAAGATATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (142-203)

• SLBB domain (SLBB) (63-118)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	100	100	1
comEA	Staphylococcus aureus MW2	37.273	100	0.4
comEA	Staphylococcus aureus N315	36.364	100	0.39