Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYA   Type   Machinery gene
Locus tag   DQN23_RS00705 Genome accession   NZ_LS483403
Coordinates   105164..106105 (+) Length   313 a.a.
NCBI ID   WP_020915987.1    Uniprot ID   -
Organism   Streptococcus lutetiensis strain NCTC13774     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 100164..111105
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DQN23_RS00695 (NCTC13774_00140) rpoC 100929..104567 (+) 3639 WP_020915985.1 DNA-directed RNA polymerase subunit beta' -
  DQN23_RS00700 (NCTC13774_00141) - 104724..105092 (+) 369 WP_020915986.1 DUF1033 family protein -
  DQN23_RS00705 (NCTC13774_00142) comYA 105164..106105 (+) 942 WP_020915987.1 competence type IV pilus ATPase ComGA Machinery gene
  DQN23_RS00710 (NCTC13774_00143) comYB 106029..107072 (+) 1044 WP_020915988.1 competence type IV pilus assembly protein ComGB Machinery gene
  DQN23_RS00715 (NCTC13774_00144) comYC 107072..107365 (+) 294 WP_020915989.1 competence type IV pilus major pilin ComGC Machinery gene
  DQN23_RS00720 (NCTC13774_00145) comYD 107349..107780 (+) 432 WP_020915990.1 competence type IV pilus minor pilin ComGD Machinery gene
  DQN23_RS00725 (NCTC13774_00146) comYE 107734..108027 (+) 294 WP_020915991.1 competence type IV pilus minor pilin ComGE Machinery gene
  DQN23_RS00730 (NCTC13774_00147) comYF 108011..108448 (+) 438 WP_043894883.1 competence type IV pilus minor pilin ComGF Machinery gene
  DQN23_RS00735 (NCTC13774_00148) comGG 108420..108770 (+) 351 WP_020915993.1 competence type IV pilus minor pilin ComGG -
  DQN23_RS00740 (NCTC13774_00149) comYH 108824..109780 (+) 957 WP_020915994.1 class I SAM-dependent methyltransferase Machinery gene
  DQN23_RS00745 (NCTC13774_00150) - 109834..111033 (+) 1200 WP_020915995.1 acetate kinase -

Sequence

Protein


Download         Length: 313 a.a.        Molecular weight: 35692.88 Da        Isoelectric Point: 5.9480

>NTDB_id=1140322 DQN23_RS00705 WP_020915987.1 105164..106105(+) (comYA) [Streptococcus lutetiensis strain NCTC13774]
MIQEKAKQLIKDAVEKNVQDIYIVPKKEHYEIYQRVGDERQFIQEVASDEMTSVISHFKFVAGMNVGEKRRSQLGSCDYE
FAEGEEISLRLSTVGDYRGRESLVIRLLYSGRHDLQYWFDGMKNILEAIDSRGLYLFSGPVGSGKTTLMYQLVREKFPDK
QVITIEDPVEIKQDNMLQLQLNDEIGMTYDNLIKLSLRHRPDILIIGEIRDSETARAVIRASLTGVLVFSTIHAKSIPGV
YARLLELGVSRQELENSLRAIIYQRLIGGGGIVDFAKGDFEHYPPTRWNEQIKSLARDGHIGLEEAKIEKIAL

Nucleotide


Download         Length: 942 bp        

>NTDB_id=1140322 DQN23_RS00705 WP_020915987.1 105164..106105(+) (comYA) [Streptococcus lutetiensis strain NCTC13774]
ATGATTCAGGAAAAAGCAAAGCAGCTGATTAAGGATGCTGTTGAAAAGAATGTGCAAGATATTTACATTGTTCCTAAGAA
GGAGCACTATGAAATTTATCAACGTGTTGGAGATGAACGTCAGTTTATCCAAGAAGTTGCTAGTGATGAAATGACAAGCG
TGATTAGTCACTTTAAATTTGTTGCGGGAATGAATGTTGGTGAGAAACGTCGTAGTCAGCTAGGGTCTTGTGATTATGAA
TTTGCTGAGGGTGAAGAGATTTCACTGCGTTTATCCACTGTTGGTGATTATCGCGGGCGTGAGAGCCTGGTCATCAGACT
TCTTTATTCAGGTCGTCATGATTTGCAATATTGGTTCGATGGGATGAAGAATATTTTAGAGGCGATTGATAGTCGAGGCC
TTTATCTTTTTTCCGGGCCGGTAGGGAGTGGAAAGACTACTCTCATGTATCAATTGGTTCGTGAAAAGTTCCCCGATAAG
CAGGTCATTACGATAGAAGACCCTGTTGAGATTAAGCAGGATAATATGTTGCAGCTGCAGCTTAATGATGAAATTGGCAT
GACTTATGATAATCTCATTAAACTCTCGCTTCGCCATAGACCTGATATTTTAATTATTGGAGAGATTCGTGATAGTGAGA
CAGCGCGTGCGGTTATTAGAGCTAGTCTAACAGGTGTGCTCGTCTTTTCAACTATTCATGCGAAAAGTATCCCAGGTGTT
TATGCCAGATTATTGGAGTTGGGAGTTAGTAGACAAGAGCTCGAAAATAGTTTACGAGCGATTATTTATCAACGATTAAT
TGGCGGTGGAGGTATTGTGGATTTTGCCAAAGGAGATTTTGAACACTATCCCCCAACAAGGTGGAATGAGCAAATTAAAA
GCCTTGCTAGGGACGGACATATTGGCCTTGAGGAAGCAAAAATCGAAAAAATTGCCCTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(8-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYA Streptococcus mutans UA159

71.061

99.361

0.706
  comYA Streptococcus mutans UA140

70.74

99.361

0.703
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

67.097

99.042

0.665
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

65.064

99.681

0.649
  comYA Streptococcus gordonii str. Challis substr. CH1

64.952

99.361

0.645
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

64.423

99.681

0.642
  comGA/cglA/cilD Streptococcus pneumoniae D39

64.423

99.681

0.642
  comGA/cglA/cilD Streptococcus pneumoniae R6

64.423

99.681

0.642
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

64.423

99.681

0.642
  comGA Lactococcus lactis subsp. cremoris KW2

53.994

100

0.54
  comGA Latilactobacillus sakei subsp. sakei 23K

43.066

87.54

0.377

Multiple sequence alignment