Detailed information NTG1132274

Bioinformatically predicted

Overview

• Name: comGF
• Type: Machinery gene
• Locus tag: BKMNNEJI_RS02425
• Genome accession: NZ_LR822061
• Coordinates: 452022..452519 (+)
• Length: 165 a.a.
• NCBI ID: WP_001788897.1
• Uniprot ID: -
• Organism: Staphylococcus aureus isolate HU-14
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 447022..457519

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BKMNNEJI_RS02385 (LJDFIFNA_00447)	-	447066..448052 (+)	987	WP_000161309.1	ROK family glucokinase	-
BKMNNEJI_RS02390 (LJDFIFNA_00448)	-	448052..448381 (+)	330	WP_001018871.1	MTH1187 family thiamine-binding protein	-
BKMNNEJI_RS02395 (LJDFIFNA_00449)	-	448378..449001 (+)	624	WP_001223008.1	MBL fold metallo-hydrolase	-
BKMNNEJI_RS02400 (LJDFIFNA_00450)	comGA	449053..450027 (+)	975	WP_000697220.1	competence type IV pilus ATPase ComGA	Machinery gene
BKMNNEJI_RS02405 (LJDFIFNA_00451)	comGB	449999..451069 (+)	1071	WP_000776422.1	competence type IV pilus assembly protein ComGB	Machinery gene
BKMNNEJI_RS02410 (LJDFIFNA_00452)	comGC	451083..451394 (+)	312	WP_000472256.1	competence type IV pilus major pilin ComGC	Machinery gene
BKMNNEJI_RS02415 (LJDFIFNA_00453)	comGD	451372..451818 (+)	447	WP_001788899.1	competence type IV pilus minor pilin ComGD	Machinery gene
BKMNNEJI_RS02420 (LJDFIFNA_00454)	comGE	451805..452104 (+)	300	WP_000844410.1	hypothetical protein	Machinery gene
BKMNNEJI_RS02425 (LJDFIFNA_00455)	comGF	452022..452519 (+)	498	WP_001788897.1	competence type IV pilus minor pilin ComGF	Machinery gene
BKMNNEJI_RS02430 (LJDFIFNA_RS02430)	-	452616..452762 (+)	147	WP_001792109.1	hypothetical protein	-
BKMNNEJI_RS02435 (LJDFIFNA_00456)	-	452752..453276 (+)	525	WP_001015120.1	shikimate kinase	-
BKMNNEJI_RS02440 (LJDFIFNA_00457)	gcvT	453435..454526 (+)	1092	WP_000093349.1	glycine cleavage system aminomethyltransferase GcvT	-
BKMNNEJI_RS02445 (LJDFIFNA_00458)	gcvPA	454546..455892 (+)	1347	WP_000019691.1	aminomethyl-transferring glycine dehydrogenase subunit GcvPA	-
BKMNNEJI_RS02450 (LJDFIFNA_00459)	gcvPB	455885..457357 (+)	1473	WP_000202188.1	aminomethyl-transferring glycine dehydrogenase subunit GcvPB	-

Sequence

Protein

• Length: 165 a.a.
• Molecular weight: 19181.99 Da
• Isoelectric Point: 10.1964

>NTDB_id=1132274 BKMNNEJI_RS02425 WP_001788897.1 452022..452519(+) (comGF) [Staphylococcus aureus isolate HU-14]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISIILLIVPDLIRLSKTFLIESRELTTVDFEFFSRDI
LEDFKGVDKNDIEIRQQRIILHKGEEMIEYKLINNKIIKVVNDRGNITMINNVTAFTANIYYKSIIKITITVKVGTNLQT
KTIYV

Nucleotide

• Length: 498 bp

>NTDB_id=1132274 BKMNNEJI_RS02425 WP_001788897.1 452022..452519(+) (comGF) [Staphylococcus aureus isolate HU-14]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTTATTGAAATGTTAGTAGCGATGATGGTTATAAGTATAATTTTACTAATTGTTCCAGACT
TAATTAGACTTAGCAAAACTTTTCTAATTGAAAGTAGGGAATTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGAGGATTTTAAAGGTGTAGATAAAAACGATATTGAAATTAGGCAACAGCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATAAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACAATGATTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCTATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATCTGCAGACT
AAAACTATTTATGTATAA

Domains

Predicted by InterproScan.

• Prokaryotic N-terminal methylation motif (N_methyl) (28-51)

• Putative Competence protein ComGF (ComGF) (69-155)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGF	Staphylococcus aureus N315	100	100	1
comGF	Staphylococcus aureus MW2	96.364	100	0.964