Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   ACAK34_RS16115 Genome accession   NZ_AP031582
Coordinates   3332369..3333169 (-) Length   266 a.a.
NCBI ID   WP_004738086.1    Uniprot ID   -
Organism   Acinetobacter baumannii strain JUNP499     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3327369..3338169
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACAK34_RS16095 (JUNP499_3228) - 3328434..3329132 (-) 699 WP_000058787.1 tetratricopeptide repeat protein -
  ACAK34_RS16100 (JUNP499_3229) hisS 3329161..3330453 (-) 1293 WP_000095261.1 histidine--tRNA ligase -
  ACAK34_RS16105 (JUNP499_3230) ispG 3330450..3331565 (-) 1116 WP_000572095.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  ACAK34_RS16110 (JUNP499_3231) - 3331587..3332378 (-) 792 WP_000405163.1 RodZ domain-containing protein -
  ACAK34_RS16115 (JUNP499_3232) pilF 3332369..3333169 (-) 801 WP_004738086.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  ACAK34_RS16120 (JUNP499_3233) rlmN 3333185..3334417 (-) 1233 WP_000093085.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  ACAK34_RS16125 (JUNP499_3234) ndk 3334540..3334971 (-) 432 WP_000963851.1 nucleoside-diphosphate kinase -
  ACAK34_RS16130 (JUNP499_3235) iscX 3335085..3335285 (-) 201 WP_000524329.1 Fe-S cluster assembly protein IscX -
  ACAK34_RS16135 (JUNP499_3236) - 3335385..3335936 (-) 552 WP_000669568.1 phosphatase PAP2 family protein -
  ACAK34_RS16140 (JUNP499_3237) - 3335961..3337259 (-) 1299 WP_086225874.1 glycosyltransferase family 1 protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30094.44 Da        Isoelectric Point: 9.2307

>NTDB_id=110374 ACAK34_RS16115 WP_004738086.1 3332369..3333169(-) (pilF) [Acinetobacter baumannii strain JUNP499]
MSTPKLKIMLCMGVAVALLASGCQTSQTVKKDPEKAVKVRTQLAAEYIRSGDLDSAKRSLDQALSVDSRDATANMMMGIL
LQQEGSKPNLEKAEHYFKRAISSEPDNAQARNNYGTYLYQMERYNDAIEQFRIAGATLGYDQRYQALENLGRIYLKLGDI
ASAEKTFKQALLANRDSYISMLELAEIFYLQQQIPAATQMYEQYVRTVGQKNQGARALWIGLRVARANADKMGMQVLVNQ
LRALFPESPEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=110374 ACAK34_RS16115 WP_004738086.1 3332369..3333169(-) (pilF) [Acinetobacter baumannii strain JUNP499]
TTGAGTACTCCGAAGTTGAAGATCATGTTATGTATGGGGGTTGCTGTTGCACTTTTGGCAAGTGGTTGTCAGACTTCGCA
AACGGTTAAAAAGGACCCGGAAAAAGCAGTAAAAGTGCGTACGCAGCTTGCTGCCGAATACATTCGTTCTGGTGACTTGG
ATTCTGCAAAGCGTTCTCTTGACCAAGCGTTAAGTGTCGATAGCCGTGATGCAACGGCAAATATGATGATGGGTATTTTA
TTGCAACAAGAAGGCAGTAAACCCAATTTAGAAAAAGCGGAACATTACTTTAAGCGTGCAATTTCTTCTGAACCAGACAA
TGCTCAGGCACGTAATAATTATGGTACTTATTTGTACCAAATGGAGCGTTATAATGATGCTATTGAGCAATTTCGTATTG
CTGGTGCAACATTAGGCTATGATCAACGTTACCAGGCTTTAGAAAATCTGGGGCGAATTTATTTAAAACTAGGCGATATT
GCGAGTGCTGAAAAAACATTCAAACAAGCACTTCTTGCTAACCGCGACTCTTACATTTCAATGTTAGAGTTAGCAGAAAT
TTTTTATTTGCAGCAGCAGATTCCGGCTGCTACACAAATGTATGAACAATATGTTCGTACAGTGGGGCAGAAAAATCAGG
GTGCACGTGCACTTTGGATTGGTTTAAGAGTGGCTCGAGCCAATGCGGATAAAATGGGAATGCAAGTTTTAGTAAACCAG
TTACGTGCTTTATTCCCGGAAAGTCCAGAATATCAACGTTATTTGCAATTACAGTATAGTACTGAGGCCGTATGGAAATA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(39-68)

Tetratricopeptide repeat (TPR_16)

(89-134)

Tetratricopeptide repeat (TPR_16)

(149-206)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

100

100

1
  pilF Acinetobacter baylyi ADP1

74.231

97.744

0.726

Multiple sequence alignment