Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   ACKW6F_RS11630 Genome accession   NZ_CP176527
Coordinates   2301398..2302468 (-) Length   356 a.a.
NCBI ID   WP_025092972.1    Uniprot ID   A0A5C0WKU5
Organism   Bacillus safensis strain D-62     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2296398..2307468
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACKW6F_RS11580 (ACKW6F_11580) sipW 2296492..2297064 (-) 573 WP_024422971.1 signal peptidase I SipW -
  ACKW6F_RS11585 (ACKW6F_11585) tapA 2297117..2297644 (-) 528 WP_024422972.1 amyloid fiber anchoring/assembly protein TapA -
  ACKW6F_RS11590 (ACKW6F_11590) - 2297944..2298222 (+) 279 WP_171464609.1 DUF3889 domain-containing protein -
  ACKW6F_RS11595 (ACKW6F_11595) - 2298257..2298451 (-) 195 WP_149126478.1 YqzE family protein -
  ACKW6F_RS11600 (ACKW6F_11600) - 2298511..2298894 (-) 384 WP_073206276.1 competence type IV pilus minor pilin ComGG -
  ACKW6F_RS11605 (ACKW6F_11605) comGF 2298891..2299418 (-) 528 WP_335343444.1 competence type IV pilus minor pilin ComGF -
  ACKW6F_RS11610 (ACKW6F_11610) comGE 2299330..2299644 (-) 315 WP_149126477.1 competence type IV pilus minor pilin ComGE -
  ACKW6F_RS11615 (ACKW6F_11615) comGD 2299628..2300074 (-) 447 WP_149126476.1 competence type IV pilus minor pilin ComGD -
  ACKW6F_RS11620 (ACKW6F_11620) comGC 2300067..2300360 (-) 294 WP_024422977.1 competence type IV pilus major pilin ComGC Machinery gene
  ACKW6F_RS11625 (ACKW6F_11625) comGB 2300377..2301417 (-) 1041 WP_103131948.1 competence type IV pilus assembly protein ComGB -
  ACKW6F_RS11630 (ACKW6F_11630) comGA 2301398..2302468 (-) 1071 WP_025092972.1 competence type IV pilus ATPase ComGA Machinery gene
  ACKW6F_RS11635 (ACKW6F_11635) - 2302649..2303524 (-) 876 WP_024422979.1 STAS domain-containing protein -
  ACKW6F_RS11645 (ACKW6F_11645) - 2303748..2304128 (-) 381 WP_007501232.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  ACKW6F_RS11650 (ACKW6F_11650) - 2304342..2304584 (+) 243 WP_003217440.1 DUF2626 domain-containing protein -
  ACKW6F_RS11655 (ACKW6F_11655) - 2304632..2305270 (-) 639 WP_024422981.1 MBL fold metallo-hydrolase -
  ACKW6F_RS11660 (ACKW6F_11660) - 2305428..2305601 (+) 174 WP_003217333.1 DUF2759 domain-containing protein -
  ACKW6F_RS11665 (ACKW6F_11665) - 2305826..2306140 (-) 315 WP_024427984.1 MTH1187 family thiamine-binding protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40412.86 Da        Isoelectric Point: 9.5360

>NTDB_id=1080684 ACKW6F_RS11630 WP_025092972.1 2301398..2302468(-) (comGA) [Bacillus safensis strain D-62]
MYGIEYLRQELLEEACRMRASDVHIVPKEKEASVSFRVDSDLIQQRTIDKKSGERLIAHFKFLSSMDIGEKRRPQNGSLA
VMLRKGQVFIRMSTLPTVNDESLVIRILPQDHVPKIKHLSLFPKASARLLSFLNHSHGLILFTGPTNSGKTTTLYSLIQF
AKKNFNRNIITLEDPVETRNEEVLQVQVNEKAGITYAAGLRAILRHDPDVIVLGEIRDAETARTAIRAALTGHLVLSTLH
AKNAKGALYRMLEFGVTMNELEQTMVAIAAQRLIELTCPFCGERCQLYCKLNRPIRRTNVFELLFGKELGECIKEAKGEY
AHSSYETLQRLIRKGVALGYLSKNTYHRWVYEEASL

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=1080684 ACKW6F_RS11630 WP_025092972.1 2301398..2302468(-) (comGA) [Bacillus safensis strain D-62]
TTGTATGGGATTGAATATTTAAGGCAAGAGCTGCTAGAAGAAGCATGCCGCATGAGGGCTTCTGATGTGCATATTGTGCC
AAAAGAAAAGGAAGCTTCGGTGTCATTTCGTGTTGACTCAGATTTAATCCAGCAGCGAACGATTGATAAAAAAAGCGGAG
AGAGGCTTATTGCACATTTTAAATTTTTATCTTCTATGGATATCGGGGAAAAAAGGAGACCGCAAAATGGCTCACTAGCT
GTGATGCTAAGAAAAGGACAAGTGTTTATTCGGATGTCTACTTTGCCGACAGTAAATGATGAGAGCTTAGTGATTAGAAT
ATTGCCGCAGGATCATGTTCCGAAAATAAAACATCTATCATTATTTCCGAAAGCCTCAGCCAGATTATTATCCTTTTTAA
ACCACTCGCATGGACTCATTTTATTTACTGGGCCAACCAATTCGGGGAAAACAACAACCCTCTATTCACTGATCCAGTTT
GCAAAAAAGAATTTCAACCGAAATATTATTACACTTGAAGATCCTGTGGAAACAAGAAATGAAGAAGTATTACAGGTTCA
AGTAAATGAAAAAGCCGGCATCACATATGCCGCAGGATTACGCGCTATTTTAAGACATGATCCAGATGTGATTGTGCTAG
GAGAAATAAGAGATGCTGAAACGGCTAGAACGGCGATTAGAGCAGCACTGACAGGCCATTTAGTATTGAGTACGCTCCAT
GCAAAAAACGCAAAAGGAGCCCTTTATCGTATGCTTGAATTTGGTGTCACGATGAATGAACTTGAGCAAACGATGGTTGC
GATTGCTGCGCAGCGATTAATCGAGCTCACCTGTCCTTTTTGCGGAGAAAGATGTCAGCTTTACTGTAAATTAAATCGAC
CGATCAGACGAACAAATGTATTTGAACTGCTGTTCGGAAAAGAGCTTGGTGAGTGTATCAAGGAGGCTAAAGGAGAATAT
GCTCACTCGTCATATGAAACACTGCAAAGATTAATTCGTAAAGGAGTGGCACTCGGCTATTTATCAAAAAACACTTACCA
TCGCTGGGTTTATGAAGAAGCGAGCCTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5C0WKU5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

64.689

99.438

0.643

Multiple sequence alignment