Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   ACHZDB_RS08900 Genome accession   NZ_CP172405
Coordinates   1969652..1970668 (-) Length   338 a.a.
NCBI ID   WP_167425522.1    Uniprot ID   -
Organism   Vibrio campbellii strain LB503     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1964652..1975668
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACHZDB_RS08875 (ACHZDB_08875) aroK 1965512..1966030 (-) 519 WP_010443904.1 shikimate kinase AroK -
  ACHZDB_RS08880 (ACHZDB_08880) pilQ 1966219..1967967 (-) 1749 WP_045369316.1 type IV pilus secretin PilQ Machinery gene
  ACHZDB_RS08885 (ACHZDB_08885) pilP 1968002..1968517 (-) 516 WP_005432702.1 pilus assembly protein PilP Machinery gene
  ACHZDB_RS08890 (ACHZDB_08890) pilO 1968507..1969100 (-) 594 WP_005432718.1 type 4a pilus biogenesis protein PilO Machinery gene
  ACHZDB_RS08895 (ACHZDB_08895) pilN 1969093..1969668 (-) 576 WP_005432710.1 PilN domain-containing protein Machinery gene
  ACHZDB_RS08900 (ACHZDB_08900) pilM 1969652..1970668 (-) 1017 WP_167425522.1 type IV pilus biogenesis protein PilM Machinery gene
  ACHZDB_RS08905 (ACHZDB_08905) - 1970847..1973414 (+) 2568 WP_038889585.1 penicillin-binding protein 1A -
  ACHZDB_RS08910 (ACHZDB_08910) oxyR 1973492..1974400 (-) 909 WP_005432705.1 DNA-binding transcriptional regulator OxyR -
  ACHZDB_RS08915 (ACHZDB_08915) - 1974575..1975303 (+) 729 WP_399423791.1 redoxin family protein -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 37874.32 Da        Isoelectric Point: 6.3141

>NTDB_id=1067448 ACHZDB_RS08900 WP_167425522.1 1969652..1970668(-) (pilM) [Vibrio campbellii strain LB503]
MDKLLVTGIDIGHNSLKAVVLKPNGNSYALLGYKEIILKEGIVAENNTINHQEIVKTLKEMKKELPFRARKVAISVPDNA
VISKKLQIEQNLEETEKEFAVVQAFSHQSPFPVEELSLDFIKVDTDEQMRGTDSYQVFATRKDVVETRVEALQKSGLKPV
LVDVHSQSLGHMWKLVAERFPEKNKYCLLDIGSLNSSFTMFTEQGELFHKEFPCGTRTTGSLFQQDPLADDAQVATEQFN
RQIVERVKRQMQLYTSINGSQDIKGVWLSGEGAATPMLAEELSSQLALECELLNPLGLFEMKVSKRKRRAADWQHFTAAA
GLAVRGIQWLGGDNAVSH

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=1067448 ACHZDB_RS08900 WP_167425522.1 1969652..1970668(-) (pilM) [Vibrio campbellii strain LB503]
ATGGATAAGCTATTAGTTACGGGCATAGATATTGGCCATAACAGCCTCAAAGCCGTGGTACTTAAACCCAATGGCAACAG
TTACGCACTACTGGGTTACAAAGAAATAATACTTAAGGAAGGTATTGTCGCTGAAAATAACACCATAAATCATCAAGAAA
TTGTAAAGACCCTTAAAGAGATGAAAAAAGAGCTACCTTTTCGTGCTCGAAAGGTCGCAATTTCAGTTCCAGATAATGCT
GTGATCAGTAAAAAGCTGCAGATAGAGCAGAACTTAGAAGAGACTGAAAAGGAGTTTGCTGTTGTGCAAGCCTTCTCACA
TCAATCTCCATTCCCCGTGGAAGAGCTGAGTCTTGACTTCATCAAGGTTGATACGGATGAGCAGATGCGAGGCACTGACA
GTTATCAGGTCTTCGCGACTCGTAAAGACGTGGTAGAAACCCGAGTTGAGGCATTGCAGAAATCAGGCTTGAAACCAGTA
CTGGTGGACGTGCACTCACAAAGTCTTGGTCATATGTGGAAATTGGTAGCGGAACGTTTTCCTGAAAAGAACAAGTATTG
TTTACTGGACATTGGTTCTTTGAACAGTTCTTTCACTATGTTCACTGAACAAGGTGAATTGTTTCATAAAGAATTTCCCT
GCGGTACTCGCACTACGGGCAGTTTGTTTCAACAAGATCCATTAGCAGACGATGCACAAGTGGCAACGGAGCAATTTAAT
CGACAAATCGTTGAGCGTGTTAAACGTCAGATGCAGCTCTATACCTCAATTAATGGAAGCCAAGATATCAAAGGCGTTTG
GCTTTCGGGAGAGGGCGCTGCAACACCTATGTTGGCCGAGGAGCTGTCTAGCCAGTTGGCACTAGAGTGTGAACTGCTTA
ATCCACTTGGTTTGTTTGAAATGAAGGTGTCTAAACGCAAACGTCGTGCCGCGGATTGGCAACACTTCACTGCTGCAGCC
GGACTGGCTGTACGTGGTATTCAGTGGCTAGGAGGTGACAATGCTGTATCACATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio campbellii strain DS40M4

97.929

100

0.979
  pilM Vibrio cholerae strain A1552

49.415

100

0.5

Multiple sequence alignment