Detailed information NTG1035385

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: ACA343_RS28810
• Genome accession: NZ_CP166821
• Coordinates: 6163901..6164965 (-)
• Length: 354 a.a.
• NCBI ID: WP_003110888.1
• Uniprot ID: -
• Organism: Pseudomonas aeruginosa strain J5083553
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 6158901..6169965

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACA343_RS28785 (ACA343_28785)	aroK	6159436..6159954 (-)	519	WP_003095833.1	shikimate kinase AroK	-
ACA343_RS28790 (ACA343_28790)	pilQ	6159966..6162110 (-)	2145	WP_015503770.1	type 4a pilus secretin PilQ	Machinery gene
ACA343_RS28795 (ACA343_28795)	pilP	6162164..6162688 (-)	525	WP_003095836.1	type 4a pilus biogenesis lipoprotein PilP	-
ACA343_RS28800 (ACA343_28800)	pilO	6162685..6163308 (-)	624	WP_003103268.1	type 4a pilus biogenesis protein PilO	-
ACA343_RS28805 (ACA343_28805)	pilN	6163305..6163901 (-)	597	WP_003095839.1	type 4a pilus biogenesis protein PilN	-
ACA343_RS28810 (ACA343_28810)	pilM	6163901..6164965 (-)	1065	WP_003110888.1	type IV pilus assembly protein PilM	Machinery gene
ACA343_RS28815 (ACA343_28815)	-	6165150..6167621 (+)	2472	WP_019484455.1	penicillin-binding protein 1A	-
ACA343_RS28820 (ACA343_28820)	-	6167717..6168985 (-)	1269	WP_003095842.1	malic enzyme	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 37983.48 Da
• Isoelectric Point: 4.5405

>NTDB_id=1035385 ACA343_RS28810 WP_003110888.1 6163901..6164965(-) (pilM) [Pseudomonas aeruginosa strain J5083553]
MLGLIKKKANTLLGIDISSTSVKLLELSRSGGRYKVEAYAVEPLPPNAVVEKNIVELEGVGQALSRVLVKAKTNLKSAVV
AVAGSAVITKTIEMEAGLSEDELENQLKIEADQYIPYPLEEVAIDFEVQGLSARNPERVDVLLAACRKENVEVREAALAL
AGLTAKVVDVEAYALERSYALLSSQLGADTDQLTVAVVDIGATMTTLSVLHNGRTIYTREQLFGGRQLTEEIQRRYGLSV
EEAGLAKKQGGLPDDYDSEVLRPFKDAVVQQVSRSLQFFFAAGQFNDVDYIVLAGGTASIQDLDRLIQQKIGTPTLVANP
FADMALNGKVNAGALASDAPALMIACGLALRSFD

Nucleotide

• Length: 1065 bp

>NTDB_id=1035385 ACA343_RS28810 WP_003110888.1 6163901..6164965(-) (pilM) [Pseudomonas aeruginosa strain J5083553]
GTGCTAGGGCTCATAAAGAAGAAAGCGAACACCTTGCTGGGGATCGACATCAGCTCGACCTCGGTAAAGCTCCTTGAGTT
GAGCCGCTCCGGAGGCCGCTACAAAGTGGAGGCCTATGCCGTCGAGCCGCTTCCGCCGAATGCAGTCGTGGAAAAGAACA
TCGTCGAGCTGGAAGGGGTCGGCCAGGCGTTGTCACGGGTGCTGGTCAAGGCGAAAACCAACCTGAAGTCGGCCGTGGTC
GCCGTCGCCGGTTCGGCGGTGATCACCAAGACCATCGAGATGGAGGCCGGGCTTTCCGAGGATGAACTGGAAAACCAGCT
GAAGATCGAGGCCGACCAGTACATCCCCTATCCGCTGGAGGAAGTCGCCATCGACTTCGAGGTCCAGGGACTCTCCGCGC
GCAATCCCGAGCGCGTGGACGTACTCCTGGCCGCCTGTCGGAAAGAGAACGTCGAGGTTCGCGAAGCGGCCCTGGCGCTG
GCCGGCCTGACCGCCAAGGTGGTCGACGTCGAGGCCTACGCGCTGGAGCGCTCCTATGCGCTGCTGAGCAGCCAGCTGGG
TGCCGACACCGACCAGTTGACCGTGGCGGTAGTGGACATCGGCGCGACCATGACCACCCTGAGCGTCCTGCACAACGGAC
GCACCATCTATACCCGCGAACAGCTGTTCGGCGGTCGCCAGCTCACCGAGGAGATCCAGCGGCGTTACGGACTCTCGGTG
GAGGAAGCCGGTCTCGCCAAGAAGCAGGGTGGTCTTCCGGATGACTACGACAGCGAAGTTCTGCGTCCGTTCAAGGACGC
CGTGGTGCAGCAGGTTTCCCGCTCGCTGCAGTTCTTCTTCGCCGCCGGACAGTTCAACGACGTCGACTACATCGTCCTGG
CGGGCGGTACGGCGTCTATCCAGGATCTCGATCGGTTGATCCAGCAGAAGATCGGCACCCCGACCCTGGTCGCCAACCCG
TTCGCCGACATGGCGCTGAACGGCAAGGTGAATGCCGGCGCCCTGGCCAGCGACGCTCCGGCGTTGATGATCGCCTGCGG
CCTGGCGTTGAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-353)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	55.899	100	0.562
comM	Acinetobacter nosocomialis M2	55.899	100	0.562
comM	Acinetobacter baylyi ADP1	51.977	100	0.52
pilM	Legionella pneumophila strain ERS1305867	46.61	100	0.466