Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   ABEF82_RS12385 Genome accession   NZ_CP156787
Coordinates   2546391..2547182 (-) Length   263 a.a.
NCBI ID   WP_404798875.1    Uniprot ID   -
Organism   Acinetobacter thermotolerans strain ANC 7924     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2541391..2552182
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABEF82_RS12365 (ABEF82_12365) - 2542442..2543140 (-) 699 WP_347453145.1 tetratricopeptide repeat protein -
  ABEF82_RS12370 (ABEF82_12370) hisS 2543171..2544466 (-) 1296 WP_347453146.1 histidine--tRNA ligase -
  ABEF82_RS12375 (ABEF82_12375) ispG 2544470..2545591 (-) 1122 WP_347453147.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  ABEF82_RS12380 (ABEF82_12380) - 2545615..2546400 (-) 786 WP_347454713.1 RodZ domain-containing protein -
  ABEF82_RS12385 (ABEF82_12385) pilF 2546391..2547182 (-) 792 WP_404798875.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  ABEF82_RS12390 (ABEF82_12390) rlmN 2547206..2548441 (-) 1236 WP_347455985.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  ABEF82_RS12395 (ABEF82_12395) ndk 2548564..2548995 (-) 432 WP_347455986.1 nucleoside-diphosphate kinase -
  ABEF82_RS12400 (ABEF82_12400) iscX 2549106..2549306 (-) 201 WP_347456360.1 Fe-S cluster assembly protein IscX -
  ABEF82_RS12405 (ABEF82_12405) - 2549396..2549951 (-) 556 Protein_2394 phosphatase PAP2 family protein -
  ABEF82_RS12410 (ABEF82_12410) - 2550045..2550455 (-) 411 WP_347454710.1 hypothetical protein -

Sequence

Protein


Download         Length: 263 a.a.        Molecular weight: 29712.89 Da        Isoelectric Point: 9.2335

>NTDB_id=1002776 ABEF82_RS12385 WP_404798875.1 2546391..2547182(-) (pilF) [Acinetobacter thermotolerans strain ANC 7924]
MTSKIALISTICFSLALTACQTVDAGKKDPEKAIKVRTQLAVEYLKTGDLDSAKRALDEALKINSRDAQANMMMGVLLQQ
EGSKANLEKAESYFKKAISAEPKNAQARNNYGTYLYQLERYNDAIEQFEIAGATLGYDQRYRALENLGRIYLKKGDLVNA
EKSFKQALQANRDSYISMLELAEIFYLNQQFPAASKMYEQFVHAVGQKNQGARALWIGIRTARASGDQLGMQVLVNQLRA
LFPESQEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 792 bp        

>NTDB_id=1002776 ABEF82_RS12385 WP_404798875.1 2546391..2547182(-) (pilF) [Acinetobacter thermotolerans strain ANC 7924]
ATGACATCTAAAATTGCTTTAATTTCAACAATATGTTTTTCACTTGCATTAACTGCCTGTCAGACAGTAGACGCGGGGAA
AAAGGACCCGGAAAAGGCAATTAAGGTACGTACTCAATTGGCGGTTGAATACCTGAAAACAGGTGATCTGGATTCAGCCA
AGCGCGCCCTGGACGAAGCACTGAAAATCAATTCACGTGATGCCCAGGCCAATATGATGATGGGCGTGCTGCTACAACAG
GAAGGCAGTAAAGCCAATCTGGAAAAAGCGGAAAGTTATTTTAAAAAAGCGATTTCTGCTGAGCCGAAAAATGCACAGGC
GCGTAACAATTACGGCACCTATTTATATCAACTTGAACGTTATAATGACGCGATTGAACAGTTTGAAATTGCCGGTGCCA
CCCTGGGCTATGACCAGCGTTACCGTGCCCTGGAAAATCTGGGGCGGATTTACCTGAAAAAAGGGGATCTGGTAAATGCT
GAAAAATCATTCAAACAGGCACTACAAGCCAATCGCGATTCTTATATTTCAATGTTAGAGTTGGCAGAGATTTTTTATCT
GAACCAGCAGTTCCCTGCAGCGAGCAAAATGTATGAACAGTTTGTGCATGCGGTAGGGCAGAAGAATCAGGGTGCTCGTG
CACTCTGGATAGGCATTCGCACAGCGCGTGCCAGTGGCGATCAACTCGGAATGCAGGTACTGGTGAATCAGCTGCGTGCA
CTTTTCCCTGAAAGCCAGGAATACCAACGTTATTTGCAATTACAGTACAGTACTGAGGCCGTATGGAAGTAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(35-65)

Tetratricopeptide repeat (TPR_8)

(142-173)

Tetratricopeptide repeat (TPR_17)

(94-126)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

78.462

98.859

0.776
  pilF Acinetobacter baylyi ADP1

70.196

96.958

0.681

Multiple sequence alignment