 ICEberg ID | 303 |
Name  | Tn916(pAM120)  |
| ICEO ID | ICEO_0000257 |
| Organism | Cloning vector pAM120 |
| Size (bp) | 18032 |
| GC content [Genome] (%) | 38.75[38.53] |
| Insertion site | AT rich regions |
| Function | tetracycline resistance |
| Species that ICE can be transferred to | - |
| Nucleotide Sequence | U49939 (complete ICE sequence in this genome) |
| Replicon | [U49939] |
| Genome coordinates | 1344..19375 |
| Putative oriT region | coordinates: 3844..3976; oriTDB id: 200001 ACTTAACCCCCCGTATCTAACAGGGGGGTACAAATCGACAGGAAACAGTCAAAAAAACATTAGAAAATCC TTTGGTTACAAGGGATTTACAAAATTTCAGCGTATGTCAAATGGGCTTTAAAAGTTGACATAC |
| Putative relaxase | - |
The graph information of Tn916(pAM120) components from U49939 | |||||
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| Complete gene list of Tn916(pAM120) from U49939 | |||||
| # | Gene | Coordinates [+/-], size (bp) | Product (GenBank annotation) | *Reannotation | |
| 1 | - | 13466..15385 [+], 1920 | tetracycline resistance protein | AR | |
| 2 | - | 22297..23157 [-], 861 | beta-lactamase | ||
 Flanking regions |
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(1) Gawron-Burke C; Clewell DB (1984). Regeneration of insertionally inactivated streptococcal DNA fragments after excision of transposon Tn916 in Escherichia coli: strategy for targeting and cloning of genes from gram-positive bacteria. J Bacteriol. 159(1):214-21. [PubMed:6330031]
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(2) Clewell DB; Flannagan SE; Ike Y; Jones JM; Gawron-Burke C (1988). Sequence analysis of termini of conjugative transposon Tn916. J Bacteriol. 170(7):3046-52. [PubMed:2838457]
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| experimental literature |