oriTDB

The information of the oriT region

oriTDB accession number	200001
Name	oriT_Tn916
Sequence Completeness	intact
oriT length	216 nt
oriT sequence	AAGCGGAAGTCGCAGGTGTGGACTGATCTTGCTGGCTGGTGTGGCAATAGCCACGCCAGC ACTTAACCCCCCGTATCTAACAGGGGGGTACAAATCGACAGGAAACAGTCAAAAAAACAT TAGAAAATCCTTTGGTTACAAGGGATTTACAAAATTTCAGCGTATGTCAAATGGGCTTTA AAAGTTGACATACGGCCTTTTTGATTGGAGGGATTT
IR (inverted repeat)	[61-76] [80-95] nt (ACTTAACCCCCCGTAT..ACAGGGGGGTACAAAT) [123-134] [139-150] nt (GAAAATCCTTTG..CAAGGGATTTAC)
Location of nic site	[135-136] nt
Conserved sequence flanking the nic site	TGG\|T
Note	_
	L:61-76;R:80-95;L:123-134;R:139-150;D:135-136

Visualization of oriT structure (The oriT was characterized experimentally)

1...5....10....15....20....25....30....35....40....45....50....55....60

1

AAGCGGAAGTCGCAGGTGTGGACTGATCTTGCTGGCTGGTGTGGCAATAGCCACGCCAGC

61

ACTTAACCCCCCGTATCTAACAGGGGGGTACAAATCGACAGGAAACAGTCAAAAAAACAT

121

TAGAAAATCCTTTGGTTACAAGGGATTTACAAAATTTCAGCGTATGTCAAATGGGCTTTA

181

AAAGTTGACATACGGCCTTTTTGATTGGAGGGATTT

Reference

[1] Rocco JM et al (2006) The integrase of the conjugative transposon Tn916 directs strand- and sequence-specific cleavage of the origin of conjugal transfer, oriT, by the endonuclease Orf20. J Bacteriol. 188(6):2207-13. [PMID:16513750]

[2] Jaworski DD et al (1995) A functional origin of transfer (oriT) on the conjugative transposon Tn916. J Bacteriol. 177(22):6644-51. [PMID:7592445]

[3] Roberts AP et al (2001) Comparison of Tn5397 from Clostridium difficile, Tn916 from Enterococcus faecalis and the CW459tet(M) element from Clostridium perfringens shows that they have similar conjugation regions but different insertion and excision modules. Microbiology. 147(Pt 5):1243-51. [PMID:11320127]

[4] Roberts AP et al (2009) A modular master on the move: the Tn916 family of mobile genetic elements. Trends Microbiol. 17(6):251-8. [PMID:19464182]

I. Information of Relaxase

ID	215
Name	Orf20_Tn916
GenBank accession number	AAB60013
Family	MOB_T
Length	329 aa
UniProt ID	Q47728
PDB ID	_
Pfam	Rep_trans [PF02486.18], Evalue: 1.80E-69, Aligned region: 96..301
Note	Endonuclease
Protein sequence [Download]	MLFDYVRIRFPTTDVQQVVENILQLKLSYFLHEDYGFYSYSEHYALGDIFVLCSHELDKG VLVELKGRGCRQFESYLLAQQRSWYEFFMDVLVAGGVMKRLDLAINDKTGILNIPVLTEK CQQEECISVFRSFKSYRSGELVRKEEKECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDI PIEDAEVKNRFEIRLKNERAYYAVRDLLVYDNPEHTAFKIINRYIRFVDKDDSKPRSDWK LNEEWAWFIGNNRERLKLTTKPEPYSFQRTLNWLSHQVAPTLKVAIKLDEINQTQVVKDI LDHAKLTDRHKQILKQQSVKEQDVITTKK

Reference

[1] Rocco JM et al (2006) The integrase of the conjugative transposon Tn916 directs strand- and sequence-specific cleavage of the origin of conjugal transfer, oriT, by the endonuclease Orf20. J Bacteriol. 188(6):2207-13. [PMID:16513750]

II. Auxiliary DNA-binding protein(s) of the relaxosome

#	ID	Name	GenBank	Length	Note
1	303	Int_Tn916	AAB60030	405 aa	Int apparently plays a dual role in the movement of the transposon from the donor to the recipient. Integrase;Accessory protein

Information of T4CP

ID	215
Name	Orf21_Tn916
SecReT4 accesion number	_
GenBank accession number	AAB60012
Family	TrwB/TraD
Length	461
UniProt ID	Q47727
PDB ID	_
Pfam	FtsK_SpoIIIE [PF01580.17], Evalue: 2.50E-07, Aligned region: 206..313 DUF87 [PF01935.16], Evalue: 4.40E-05, Aligned region: 216..352
Note	The AAA+ (ATPases Associated with a wide variety of cellular Activities) superfamily represents an ancient group of ATPases belonging to the ASCE (for additional strand, catalytic E) division of the P-loop NTPase fold.
Protein sequence [Download]	MKQRGKRIRPSGKDLVFHFTIASLLPVFLLVVGLFHVKTIQQINWQDFNLSQADKIDIPY LIISFSVAILICLLVAFVFKRVRYDTVKQLYHRQKLAKMILENKWYESEQVKTEGFFKDS AGRTKEKITYFPKMYYRLKNGLIQIRVEITLGKYQDQLLHLEKKLESGLYCELTDKELKD SYVEYTLLYDTIASRISIDEVEAKDGKLRLMKNVWWEYDKLPHMLIAGGTGGGKTYFILT LIEALLHTDSKLYILDPKNADLADLGSVMANVYYRKEDLLSCIETFYEEMMKRSEEMKQM KNYKTGKNYAYLGLPAHFLIFDEYVAFMEMLGTKENTAVMNKLKQIVMLGRQAGFFLILA CQRPDAKYLGDGIRDQFNFRVALGRMSEMGYGMMFGSDVQKDFFLKRIKGRGYVDVGTSV ISEFYTPLVPKGYDFLEEIKKLSNSRQSTQATCEAEVAGVD

Reference

[1] Rocco JM et al (2006) A modular master on the move: the Tn916 family of mobile genetic elements. J Bacteriol. 188(6):2207-13. [PMID:16513750]

Information of mobile element

ID	6
Element type	ICE (Conjugative transposon)
ICE description	Tn916
ICEberg accesion number	45
GenBank accession number	U09422.1
Family	Tn916
Genome size	18032 bp
Coordinate of oriT [+/-]	2441..2656 [+]
Drug resistance	resistance to tetracycline
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Enterococcus faecalis DS16 [1158677]

Reference

[1] Flannagan SE et al (1994) Nucleotide sequence of the 18-kb conjugative transposon Tn916 from Enterococcus faecalis. Plasmid. 32(3):350-4. [PMID:7899523]