 ICEberg ID | 103 |
Name  | Tn5397  |
| ICEO ID | ICEO_0000256 |
| Organism | Clostridium difficile 630 |
| Size (bp) | 20657 |
| GC content [Genome] (%) | 38.32[29.06] |
| Insertion site | AT rich regions |
| Function | Tetracycline resistance |
| Species that ICE can be transferred to | Bacillus subtilis; Clostridium difficile |
| Nucleotide Sequence | AM180355 (complete ICE sequence in this genome) |
| Replicon | chromosome (4290252 bp, BioProject:78) [NC_009089] |
| Genome coordinates | 585384..606040 |
| Putative oriT region | - |
| Putative relaxase | coordinates: 588010..589218; Locus tag: CD630_04990; Family: MOBT |
The Interaction Network among ICE/IME/CIME | ||||||
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| Detailed Informatioin of the Interaction Network | ||||||
| # | ICE | Inter_Ele [Type] | Methods | Donors | Recipients | Exper_Ref |
| 1 | Tn5397 | Tn5398 [CIME] |  in cis | - | - | - |
| This is a putative interactioin |
The graph information of Tn5397 components from AM180355 | |||||
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| Complete gene list of Tn5397 from AM180355 | |||||
| # | Gene | Coordinates [+/-], size (bp) | Product (GenBank annotation) | *Reannotation | |
| 1 | CD630_04900 | 580547..581599 [+], 1053 | putative sugar-phosphate dehydrogenase | ||
| 2 | CD630_04910 | 581655..582062 [+], 408 | PTS system, mannose/fructose/sorbose IIA component | ||
| 3 | CD630_04920 | 582091..582564 [+], 474 | PTS system, mannose/fructose/sorbose IIB component | ||
| 4 | CD630_04930 | 582611..583369 [+], 759 | PTS system, mannose/fructose/sorbose IIC component | ||
| 5 | CD630_04940 | 583393..584232 [+], 840 | PTS system, mannose/fructose/sorbose IID component | ||
| 6 | CD630_04950 | 584769..606563 [+], 21795 | Fragment of putative regulatory protein | ||
| 7 | CD630_04960 | 585695..586009 [+], 315 | putative conjugative transposon protein DUF961 family Tn5397, CTn3-Orf23 | ||
| 8 | CD630_04970 | 586031..586417 [+], 387 | putative conjugative transposon protein DUF961 family Tn5397, CTn3-Orf22 | ||
| 9 | CD630_04980 | 586451..587836 [+], 1386 | putative cell-division FtsK/SpoIIIE-family protein Tn5397, CTn3-Orf21 | Orf21_Tn, T4SS component | |
| 10 | CD630_04981 | 587839..587991 [+], 153 | putative conjugative transposon protein Tn5397,CTn3-Orf20.1 | ||
| 11 | CD630_04990 | 588010..589218 [+], 1209 | putative replication initiation factor Tn5397,CTn3-Orf20 | Relaxase, MOBT Family | |
| 12 | CD630_04991 | 589261..589482 [+], 222 | putative conjugative transposon protein Tn5397,CTn3-Orf19 | Orf19_Tn, T4SS component | |
| 13 | CD630_05000 | 589599..590096 [+], 498 | putative antirestriction protein Tn5397,CTn3-Orf18 | ||
| 14 | CD630_05010 | 590184..590576 [+], 393 | putative conjugative transposon protein Tn5397,CTn3-Orf17 | Orf17_Tn, T4SS component | |
| 15 | CD630_05020 | 590560..593007 [+], 2448 | putative ATPase Tn5397, CTn3-Orf16 | Orf16_Tn, T4SS component | |
| 16 | CD630_05030 | 593007..595175 [+], 2169 | putative membrane protein Tn5397, CTn3-Orf15 | Orf15_Tn, T4SS component | |
| 17 | CD630_05040 | 595172..598820 [+], 3649 | Fragment of putative conjugative transposon protein Tn5397, CTn3-Orf14 | ||
| 18 | CD630_05060 | 596830..598659 [+], 1830 | Reverse transcriptase/maturase/endonuclease,Group II intron | ||
| 19 | CD630_05070 | 598817..599749 [+], 933 | putative conjugative transposon protein Tn5397,CTn3-Orf13 | Orf13_Tn, T4SS component | |
| 20 | CD630_05071 | 599965..600018 [+], 54 | Fragment of Tet(M) leader peptide Tn5397,CTn3-orf12 (C-terminal region) | ||
| 21 | tetM | 600034..601953 [+], 1920 | Tetracycline resistance protein Tn5397 | AR | |
| 22 | CD630_05081 | 602049..602243 [+], 195 | putative conjugative transposon protein Tn5397,CTn3-Orf6 | ||
| 23 | CD630_05090 | 602302..602655 [-], 354 | Transcriptional regulator, HTH-type Tn5397,CTn3-Orf9 | ||
| 24 | CD630_05091 | 602866..602931 [+], 66 | putative conjugative transposon protein Tn5397,CTn3-Orf10 | ||
| 25 | CD630_05100 | 603157..603582 [+], 426 | putative RNA polymerase sigma factor Tn5397,CTn3-Orf7 | ||
| 26 | CD630_05101 | 603579..603809 [+], 231 | putative conjugative transposon protein Tn5397,CTn3-Orf8 | ||
| 27 | CD630_05102 | 603931..604068 [-], 138 | Fragment of putative conjugative transposon protein Tn5397, CTn3-Orf5 (C-terminal region) | ||
| 28 | CD630_05103 | 604190..604330 [+], 141 | putative conjugative transposon protein Tn5397,CTn3-Orf4 | ||
| 29 | tndX | 604427..606028 [+], 1602 | Recombinase site-specific resolvase family Tn5397, CTn3-Orf3 | Integrase | |
| 30 | CD630_04950 | 584769..606563 [+], 21795 | Fragment of putative regulatory protein | ||
| 31 | CD630_05130 | 606589..606777 [+], 189 | conserved hypothetical protein | ||
 Flanking regions |
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(1) Jasni AS; Mullany P; Hussain H; Roberts AP (2010). Demonstration of conjugative transposon (Tn5397)-mediated horizontal gene transfer between Clostridium difficile and Enterococcus faecalis. Antimicrob Agents Chemother. 54(11):4924-6. [PubMed:20713671]
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| (2) Sebaihia M; Wren BW; Mullany P; Fairweather NF; Minton N; Stabler R; Thomson NR; Roberts AP; Cerdeno-Tarraga AM; Wang H; Holden MT; Wright A; Churcher C; Quail MA; Baker S; Bason N; Brooks K; Chillingworth T; Cronin A; Davis P; Dowd L; Fraser A; Feltwell T; Hance Z; Holroyd S; Jagels K; Moule S; Mungall K; Price C; Rabbinowitsch E; Sharp S; Simmonds M; Stevens K; Unwin L; Whithead S; Dupuy B; Dougan G; Barrell B; Parkhill J (2006). The multidrug-resistant human pathogen Clostridium difficile has a highly mobile, mosaic genome. Nat Genet. 38(7):779-86. [PubMed:16804543] |
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(3) Wang H; Smith MC; Mullany P (2006). The conjugative transposon Tn5397 has a strong preference for integration into its Clostridium difficile target site. J Bacteriol. 188(13):4871-8. [PubMed:16788196]
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(4) Roberts AP; Johanesen PA; Lyras D; Mullany P; Rood JI (2001). Comparison of Tn5397 from Clostridium difficile, Tn916 from Enterococcus faecalis and the CW459tet(M) element from Clostridium perfringens shows that they have similar conjugation regions but different insertion and excision modules. Microbiology. 147(Pt 5):1243-51. [PubMed:11320127]
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(5) Wang H; Mullany P (2000). The large resolvase TndX is required and sufficient for integration and excision of derivatives of the novel conjugative transposon Tn5397. J Bacteriol. 182(23):6577-83. [PubMed:11073898]
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(6) Wang H; Roberts AP; Lyras D; Rood JI; Wilks M; Mullany P (2000). Characterization of the ends and target sites of the novel conjugative transposon Tn5397 from Clostridium difficile: excision and circularization is mediated by the large resolvase, TndX. J Bacteriol. 182(13):3775-83. [PubMed:10850994]
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(7) Roberts AP; Pratten J; Wilson M; Mullany P (1999). Transfer of a conjugative transposon, Tn5397 in a model oral biofilm. FEMS Microbiol Lett. 177(1):63-6. [PubMed:10436923]
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(8) Mullany P; Pallen M; Wilks M; Stephen JR; Tabaqchali S (1996). A group II intron in a conjugative transposon from the gram-positive bacterium, Clostridium difficile. Gene. 174(1):145-50. [PubMed:8863741]
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(9) Mullany P; Wilks M; Tabaqchali S (1995). Transfer of macrolide-lincosamide-streptogramin B (MLS) resistance in Clostridium difficile is linked to a gene homologous with toxin A and is mediated by a conjugative transposon, Tn5398. J Antimicrob Chemother. 35(2):305-15. [PubMed:7759394]
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| experimental literature |