Detailed information of TA system TA099998

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 4450397..4450961
• Replicon: chromosome
• Accession: NZ_CP027723
• Organism: Pseudomonas orientalis strain 8B

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: C4J98_RS20025
• Protein ID: WP_104505336.1
• Coordinates: 4450397..4450678 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A1B5E756
• Locus tag: C4J98_RS20030
• Protein ID: WP_065894994.1
• Coordinates: 4450674..4450961 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C4J98_RS20000	4445572..4446348	-	777	WP_124433735.1	hypothetical protein	-
C4J98_RS20005	4446329..4447732	-	1404	WP_124433736.1	dNTP triphosphohydrolase	-
C4J98_RS20010	4448210..4448740	+	531	WP_124433737.1	Bro-N domain-containing protein	-
C4J98_RS20015	4448840..4449553	+	714	WP_124433738.1	HNH endonuclease	-
C4J98_RS20020	4449626..4450273	+	648	WP_124433739.1	HAD-IB family hydrolase	-
C4J98_RS20025	4450397..4450678	-	282	WP_104505336.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C4J98_RS20030	4450674..4450961	-	288	WP_065894994.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
C4J98_RS20035	4451085..4451694	+	610	Protein_3845	transporter substrate-binding domain-containing protein	-
C4J98_RS20040	4451879..4452535	+	657	Protein_3846	MFS transporter	-
C4J98_RS20045	4452688..4453635	+	948	WP_124433740.1	hypothetical protein	-
C4J98_RS20050	4453743..4454123	+	381	WP_124433741.1	helix-turn-helix transcriptional regulator	-
C4J98_RS20055	4454053..4454610	-	558	WP_124433742.1	AAA family ATPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (10-77)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10944.76 Da
• Isoelectric Point: 10.3141

>T99998 WP_104505336.1 NZ_CP027723:c4450678-4450397 [Pseudomonas orientalis]
IEFSIEWDPKALKELKKLDGTIRHQFLRKLQERQDHPRVPADALYGMKDCYKIKLRGAGFRLVYRVEDDRIVILVLAVGK
RERSTVYDSAKSR

Nucleotide

• Length: 282 bp

>T99998 NZ_CP027723:c4450678-4450397 [Pseudomonas orientalis]
ATTGAGTTTAGTATTGAGTGGGATCCGAAGGCGTTAAAGGAACTCAAGAAACTGGACGGTACAATTCGTCATCAGTTTTT
GCGCAAACTTCAGGAGCGCCAAGATCACCCGCGGGTTCCAGCTGACGCGCTTTATGGAATGAAGGATTGCTACAAGATCA
AATTACGTGGTGCCGGCTTTCGGCTGGTCTACCGTGTTGAGGACGATCGAATAGTCATCCTCGTATTAGCGGTGGGTAAG
CGGGAACGTAGCACTGTCTACGATTCGGCCAAGAGCCGATAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10244.76 Da
• Isoelectric Point: 4.3557

>AT99998 WP_065894994.1 NZ_CP027723:c4450961-4450674 [Pseudomonas orientalis]
MQLVLADVAVSVSELKKNPSSVMNGANGSAVAVLNHNRVMGYMVPASVYEAMVERLDELELVQLVKERLDAKEAPIRVSL
DELIAEAEADIANGH

Nucleotide

• Length: 288 bp

>AT99998 NZ_CP027723:c4450961-4450674 [Pseudomonas orientalis]
ATGCAACTTGTTTTGGCTGACGTGGCCGTCAGCGTATCCGAACTCAAAAAGAACCCTTCATCCGTCATGAACGGAGCCAA
CGGCTCGGCGGTGGCTGTCCTCAACCATAATCGCGTGATGGGTTATATGGTCCCGGCTAGCGTATACGAGGCGATGGTTG
AGCGCCTTGATGAGCTGGAACTGGTGCAATTGGTCAAGGAAAGGCTGGACGCCAAGGAGGCACCGATTCGGGTGTCCCTT
GATGAGCTGATTGCTGAGGCAGAGGCGGATATCGCCAATGGGCATTGA

Structures

Antitoxin

• AlphaFold DB: A0A1B5E756