Detailed information of TA system TA099996

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA(toxin)
• Location: 2351395..2351996
• Replicon: chromosome
• Accession: NZ_CP027723
• Organism: Pseudomonas orientalis strain 8B

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A4U3I3Z1
• Locus tag: C4J98_RS10770
• Protein ID: WP_057722035.1
• Coordinates: 2351745..2351996 (-)
• Length: 84 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: C4J98_RS10765
• Protein ID: WP_065884693.1
• Coordinates: 2351395..2351748 (-)
• Length: 118 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C4J98_RS27240	2346824..2348461	+	1638	WP_206426489.1	hypothetical protein	-
C4J98_RS10755	2348827..2350182	+	1356	WP_124432414.1	MATE family efflux transporter	-
C4J98_RS10760	2350223..2351416	+	1194	WP_124432415.1	MFS transporter	-
C4J98_RS10765	2351395..2351748	-	354	WP_065884693.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
C4J98_RS10770	2351745..2351996	-	252	WP_057722035.1	type II toxin-antitoxin system HicA family toxin	Toxin

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (20-76)

Antitoxin

• Uncharacterised protein family HicB (55-105)

Sequences

Toxin

Protein

• Length: 84 a.a.
• Molecular weight: 9490.04 Da
• Isoelectric Point: 10.0459

>T99996 WP_057722035.1 NZ_CP027723:c2351996-2351745 [Pseudomonas orientalis]
VSKNEKLLAKLLNEHMAFTWSELVTLLRRLGYTQIEGAGSRVKFDIGDPSALITLHKPHPGNELKHYIRRQIIEQLKSGE
LIQ

Nucleotide

• Length: 252 bp

>T99996 NZ_CP027723:c2351996-2351745 [Pseudomonas orientalis]
GTGTCCAAAAATGAAAAGCTGCTCGCCAAACTGCTCAATGAGCACATGGCATTCACCTGGTCCGAACTCGTAACGCTGTT
ACGCCGACTGGGCTACACACAGATTGAAGGGGCTGGAAGCCGGGTCAAATTCGATATCGGCGACCCCAGTGCACTGATTA
CCCTGCACAAGCCTCACCCCGGCAACGAACTGAAACACTACATTCGGCGCCAAATCATCGAACAATTGAAATCAGGAGAA
CTGATTCAGTGA

Antitoxin

Protein

• Length: 118 a.a.
• Molecular weight: 12737.61 Da
• Isoelectric Point: 7.3241

>AT99996 WP_065884693.1 NZ_CP027723:c2351748-2351395 [Pseudomonas orientalis]
VNNQLKHKGYIGSIEASLEDNCLFGKILFIKALVSYEGKTVAELDAAFKEAVDDYLATCQALGQTPEKPCKGSFNVRVGH
DLHLAAALAATRKKVTLNDLTRQALNEFLQHHCPAIG

Nucleotide

• Length: 354 bp

>AT99996 NZ_CP027723:c2351748-2351395 [Pseudomonas orientalis]
GTGAACAACCAGCTCAAACACAAAGGCTACATCGGCTCCATCGAAGCCAGCCTCGAAGACAACTGCCTGTTCGGCAAAAT
CCTGTTCATCAAGGCGTTGGTGAGCTACGAAGGCAAAACCGTCGCCGAGTTGGACGCGGCTTTCAAGGAGGCGGTGGACG
ATTACCTGGCAACCTGCCAAGCCCTCGGGCAAACGCCGGAAAAGCCTTGCAAGGGCTCCTTCAACGTACGCGTGGGCCAT
GATCTGCATTTGGCGGCAGCGCTGGCGGCGACCCGTAAGAAGGTGACGTTGAACGACCTGACCCGCCAGGCGCTGAATGA
GTTTTTACAACATCATTGCCCTGCGATTGGCTGA

Structures

Toxin

• AlphaFold DB: A0A4U3I3Z1