Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 1838381..1838985 Replicon chromosome
Accession NZ_CP027723
Organism Pseudomonas orientalis strain 8B

Toxin (Protein)


Gene name hicA Uniprot ID A0A2N1E473
Locus tag C4J98_RS08445 Protein ID WP_065934378.1
Coordinates 1838381..1838569 (+) Length 63 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A1B5E431
Locus tag C4J98_RS08450 Protein ID WP_065894766.1
Coordinates 1838581..1838985 (+) Length 135 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C4J98_RS08415 1833985..1834521 + 537 WP_124432057.1 DsbE family thiol:disulfide interchange protein -
C4J98_RS08420 1834518..1834991 + 474 WP_124432058.1 cytochrome c-type biogenesis protein CcmH -
C4J98_RS08425 1834984..1836168 + 1185 WP_124432059.1 c-type cytochrome biogenesis protein CcmI -
C4J98_RS08430 1836184..1836579 + 396 WP_124432060.1 hypothetical protein -
C4J98_RS08435 1836735..1837010 - 276 WP_124432061.1 DUF3077 domain-containing protein -
C4J98_RS08440 1837686..1838252 + 567 WP_124432062.1 phosphonate metabolism protein/1,5-bisphosphokinase (PRPP-forming) PhnN -
C4J98_RS08445 1838381..1838569 + 189 WP_065934378.1 type II toxin-antitoxin system HicA family toxin Toxin
C4J98_RS08450 1838581..1838985 + 405 WP_065894766.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
C4J98_RS08455 1838982..1840178 - 1197 WP_124432063.1 MFS transporter -
C4J98_RS08460 1840466..1841470 - 1005 WP_065894770.1 sulfate ABC transporter substrate-binding protein -
C4J98_RS08465 1841567..1842391 - 825 WP_124432064.1 ion transporter -
C4J98_RS08470 1842423..1843298 - 876 WP_124432065.1 urea transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(6-59)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(3-127)


Sequences

Toxin        


Download         Length: 63 a.a.        Molecular weight: 7204.45 Da        Isoelectric Point: 11.2000

>T99994 WP_065934378.1 NZ_CP027723:1838381-1838569 [Pseudomonas orientalis]
VQSRLLIKELLEAGWVLDRVTGSHHLFTHRYNPYTIPVPHPKKDLPLGTVRSIRKRAGLFQF

Download         Length: 189 bp

>T99994 NZ_CP027723:1838381-1838569 [Pseudomonas orientalis]
GTGCAAAGCAGGCTATTGATCAAGGAGTTGCTGGAAGCGGGCTGGGTGCTGGATAGGGTCACTGGCAGCCATCACCTTTT
CACCCACCGCTATAACCCGTACACGATACCGGTGCCCCATCCGAAGAAGGATCTGCCGTTGGGCACTGTGAGAAGCATCA
GGAAACGCGCCGGGCTGTTTCAGTTTTAA

Antitoxin


Download         Length: 135 a.a.        Molecular weight: 14676.62 Da        Isoelectric Point: 4.4765

>AT99994 WP_065894766.1 NZ_CP027723:1838581-1838985 [Pseudomonas orientalis]
MQYPICIEWGDDFTATGIQIPDIPGAVTAGDSFEEAYNAAVEVAHLMLQEIAAEGGVIPMPTSVATHHAHEDYAGMGWGM
LELDISPYLGKTEKVNVTLPGYVIQRIDRYVREHNVKSRSSFLADAALEKLVRS

Download         Length: 405 bp

>AT99994 NZ_CP027723:1838581-1838985 [Pseudomonas orientalis]
ATGCAATACCCAATCTGTATCGAATGGGGCGATGACTTCACCGCCACCGGTATCCAGATCCCCGATATTCCGGGCGCCGT
CACCGCCGGGGACAGTTTCGAAGAGGCCTACAACGCGGCCGTGGAAGTGGCGCACCTCATGCTGCAGGAGATTGCCGCAG
AGGGCGGGGTGATTCCAATGCCAACCTCGGTTGCCACTCATCACGCCCATGAAGATTACGCCGGCATGGGCTGGGGTATG
TTGGAGCTGGATATCTCGCCTTATCTGGGCAAGACCGAGAAGGTCAATGTGACTTTGCCCGGTTATGTGATCCAACGCAT
CGACCGTTACGTGCGTGAACACAACGTCAAAAGCCGCTCGTCATTTCTGGCGGATGCGGCTTTGGAGAAGTTGGTGCGTT
CTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2N1E473

Antitoxin

Source ID Structure
AlphaFold DB A0A1B5E431

References