Detailed information of TA system TA099993

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 5981332..5981921
• Replicon: chromosome
• Accession: NZ_CP027722
• Organism: Pseudomonas chlororaphis subsp. aureofaciens strain C50

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: C4K11_RS26980
• Protein ID: WP_124355458.1
• Coordinates: 5981622..5981921 (-)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: C4K11_RS26975
• Protein ID: WP_124355457.1
• Coordinates: 5981332..5981625 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C4K11_RS26955	5976972..5978135	-	1164	WP_124315432.1	acyltransferase	-
C4K11_RS26960	5978330..5979520	-	1191	WP_124355455.1	serine hydrolase	-
C4K11_RS26965	5979647..5980534	+	888	WP_009051074.1	LysR family transcriptional regulator	-
C4K11_RS26970	5980807..5981199	+	393	WP_124355456.1	hypothetical protein	-
C4K11_RS26975	5981332..5981625	-	294	WP_124355457.1	putative addiction module antidote protein	Antitoxin
C4K11_RS26980	5981622..5981921	-	300	WP_124355458.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C4K11_RS26985	5982093..5982542	+	450	WP_124355459.1	DUF3828 domain-containing protein	-
C4K11_RS26990	5982567..5983577	-	1011	WP_177477863.1	NAD(P)-dependent alcohol dehydrogenase	-
C4K11_RS26995	5983635..5983934	-	300	WP_009051080.1	ArsR family transcriptional regulator	-
C4K11_RS27000	5983998..5985089	-	1092	WP_041987461.1	nitrite reductase, copper-containing	-
C4K11_RS27005	5985315..5985557	+	243	WP_007924102.1	exodeoxyribonuclease VII small subunit	-
C4K11_RS27010	5985554..5986441	+	888	WP_009051083.1	(2E,6E)-farnesyl diphosphate synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-96)

Antitoxin

• Cro/C1-type helix-turn-helix domain (47-89)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11264.11 Da
• Isoelectric Point: 10.1871

>T99993 WP_124355458.1 NZ_CP027722:c5981921-5981622 [Pseudomonas chlororaphis subsp. aureofaciens]
MIEIKQIATFMEWENRLKDPRARALIAARIFRLANGLAGDVSPVGEGVSELRIHYGPGYRVYFQPCGLQLVILLCGGDKS
RQSRDIELAKYLARRWSRS

Nucleotide

• Length: 300 bp

>T99993 NZ_CP027722:c5981921-5981622 [Pseudomonas chlororaphis subsp. aureofaciens]
ATGATCGAGATCAAACAGATCGCGACCTTTATGGAGTGGGAAAACAGGCTCAAGGATCCCAGAGCCAGGGCGTTGATCGC
TGCGCGGATTTTTCGCCTGGCCAACGGCCTTGCCGGTGACGTGTCTCCGGTGGGCGAAGGCGTCAGTGAGTTGCGCATCC
ATTACGGGCCGGGCTACCGGGTTTACTTCCAGCCGTGCGGCCTGCAACTCGTCATCCTGTTATGCGGCGGCGACAAGAGT
CGCCAGAGCCGCGATATCGAACTGGCCAAATACCTGGCCCGTCGTTGGAGTCGATCATGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10426.93 Da
• Isoelectric Point: 5.6642

>AT99993 WP_124355457.1 NZ_CP027722:c5981625-5981332 [Pseudomonas chlororaphis subsp. aureofaciens]
MNEPLFDYDPATALRDPQAIALFMADAFETGDAGYIAKALGVVARAKGMTELAHETGLSREQLYRSFSDKGNPTLKTLLA
VMRALGLELTARVHGAS

Nucleotide

• Length: 294 bp

>AT99993 NZ_CP027722:c5981625-5981332 [Pseudomonas chlororaphis subsp. aureofaciens]
ATGAACGAACCGCTATTCGACTATGACCCCGCCACGGCCTTGCGCGACCCGCAGGCCATCGCCCTGTTCATGGCGGACGC
CTTCGAGACCGGCGATGCCGGCTACATCGCCAAAGCCCTGGGCGTGGTGGCGCGGGCCAAGGGCATGACCGAACTGGCCC
ATGAAACCGGGCTGTCCCGCGAGCAGCTGTACCGCTCGTTCAGTGACAAGGGCAACCCGACCCTGAAGACCCTGCTGGCG
GTGATGCGCGCCCTGGGGCTGGAGTTGACGGCGCGGGTGCATGGGGCGTCTTGA