Detailed information of TA system TA099990

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 2323946..2324550
• Replicon: chromosome
• Accession: NZ_CP027722
• Organism: Pseudomonas chlororaphis subsp. aureofaciens strain C50

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: C4K11_RS10705
• Protein ID: WP_164485685.1
• Coordinates: 2324383..2324550 (-)
• Length: 56 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: C4K11_RS10700
• Protein ID: WP_092418556.1
• Coordinates: 2323946..2324347 (-)
• Length: 134 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C4K11_RS10685	2319826..2321715	+	1890	WP_124354773.1	PAS domain S-box protein	-
C4K11_RS10690	2321712..2322347	+	636	WP_053260452.1	response regulator transcription factor	-
C4K11_RS10695	2322441..2323205	+	765	WP_124354774.1	hypothetical protein	-
C4K11_RS10700	2323946..2324347	-	402	WP_092418556.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
C4K11_RS10705	2324383..2324550	-	168	WP_164485685.1	type II toxin-antitoxin system HicA family toxin	Toxin
C4K11_RS10710	2324928..2325209	-	282	WP_124339878.1	hypothetical protein	-
C4K11_RS10715	2325607..2325984	-	378	WP_124310792.1	hypothetical protein	-
C4K11_RS10720	2326274..2326648	-	375	Protein_2078	transposase	-
C4K11_RS10725	2326736..2329039	-	2304	WP_124354775.1	SIR2 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2326274..2326498	224

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (1-54)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-127)

Sequences

Toxin

Protein

• Length: 56 a.a.
• Molecular weight: 6192.28 Da
• Isoelectric Point: 10.4656

>T99990 WP_164485685.1 NZ_CP027722:c2324550-2324383 [Pseudomonas chlororaphis subsp. aureofaciens]
MIKELEAAGWVLERVTGSHHLFTHPYRPETVPVPHPKKDLPRGTVRAIQKLAGLI

Nucleotide

• Length: 168 bp

>T99990 NZ_CP027722:c2324550-2324383 [Pseudomonas chlororaphis subsp. aureofaciens]
TTGATCAAGGAGCTTGAAGCCGCAGGCTGGGTGCTGGAGCGCGTTACCGGCAGCCATCATCTGTTCACGCATCCCTACCG
GCCCGAGACCGTTCCGGTGCCTCACCCGAAGAAGGATCTGCCACGGGGGACGGTCAGAGCCATCCAGAAACTGGCCGGGC
TGATATAG

Antitoxin

Protein

• Length: 134 a.a.
• Molecular weight: 14588.55 Da
• Isoelectric Point: 4.7465

>AT99990 WP_092418556.1 NZ_CP027722:c2324347-2323946 [Pseudomonas chlororaphis subsp. aureofaciens]
VHYPICIEWGDEHTATGIQIPDIPGAVTAGDTFEAAYSAAIEIAHVMLEELAREGQAIPLPSPTGTHRANPDFEGMGWGM
LDIDITPYLGKTEKVNVTLPGYVIQRIDRFVREHNIKSRSSFLADAAMEKLGR

Nucleotide

• Length: 402 bp

>AT99990 NZ_CP027722:c2324347-2323946 [Pseudomonas chlororaphis subsp. aureofaciens]
GTGCACTATCCCATCTGCATCGAGTGGGGCGACGAGCACACTGCTACCGGCATCCAGATTCCTGACATTCCTGGCGCGGT
CACCGCCGGCGACACCTTCGAGGCCGCGTACAGCGCCGCCATCGAGATTGCCCACGTCATGCTCGAAGAACTGGCCCGCG
AGGGGCAAGCCATTCCCCTGCCCTCGCCCACCGGCACGCACCGCGCCAACCCCGATTTCGAGGGCATGGGCTGGGGCATG
CTGGATATCGATATCACGCCTTACCTGGGCAAGACCGAGAAGGTCAATGTGACCCTGCCGGGCTACGTGATTCAACGAAT
CGACCGCTTTGTACGGGAGCACAACATCAAGAGCCGATCGTCTTTTCTGGCAGATGCGGCGATGGAAAAGCTGGGGCGTT
AA