Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HTH_37(antitoxin)
Location 6429426..6430066 Replicon chromosome
Accession NZ_CP027720
Organism Pseudomonas chlororaphis subsp. aureofaciens strain DSM 6698

Toxin (Protein)


Gene name hicA Uniprot ID A0A285GR45
Locus tag C4K13_RS29380 Protein ID WP_028681531.1
Coordinates 6429426..6429608 (+) Length 61 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A554PIE8
Locus tag C4K13_RS29385 Protein ID WP_041984783.1
Coordinates 6429641..6430066 (+) Length 142 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C4K13_RS29350 6424746..6425453 + 708 WP_041984770.1 hypothetical protein -
C4K13_RS29355 6425450..6426373 + 924 WP_041984773.1 hypothetical protein -
C4K13_RS29360 6426370..6426918 + 549 WP_041984775.1 hypothetical protein -
C4K13_RS29365 6426915..6427619 + 705 WP_041984777.1 hypothetical protein -
C4K13_RS29370 6427592..6428563 + 972 WP_041984780.1 hypothetical protein -
C4K13_RS29375 6428560..6429201 - 642 WP_041985169.1 hypothetical protein -
C4K13_RS29380 6429426..6429608 + 183 WP_028681531.1 type II toxin-antitoxin system HicA family toxin Toxin
C4K13_RS29385 6429641..6430066 + 426 WP_041984783.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
C4K13_RS29390 6430243..6430890 - 648 WP_124355757.1 hypothetical protein -
C4K13_RS29395 6431732..6431941 + 210 WP_124355758.1 hypothetical protein -
C4K13_RS29400 6431938..6432564 + 627 WP_052443041.1 Fic family protein -
C4K13_RS29405 6433340..6433981 + 642 WP_052443043.1 lipopolysaccharide biosynthesis protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 6392412..6432564 40152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(3-58)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 61 a.a.        Molecular weight: 6764.83 Da        Isoelectric Point: 10.7304

>T99980 WP_028681531.1 NZ_CP027720:6429426-6429608 [Pseudomonas chlororaphis subsp. aureofaciens]
MKYSEFRRWLKAQGAEFQPGKGSHFKVTLNGKSTVFPDHGAKEMGEGLRKSIIKQLGLKD

Download         Length: 183 bp

>T99980 NZ_CP027720:6429426-6429608 [Pseudomonas chlororaphis subsp. aureofaciens]
ATGAAGTACAGCGAGTTTCGGCGATGGTTGAAAGCCCAAGGCGCGGAGTTCCAACCCGGCAAGGGCAGTCACTTCAAGGT
CACCTTGAATGGCAAGTCAACCGTGTTTCCAGACCACGGAGCCAAGGAAATGGGCGAAGGGTTAAGGAAGTCGATAATCA
AACAGCTGGGCCTCAAGGATTGA

Antitoxin


Download         Length: 142 a.a.        Molecular weight: 15470.58 Da        Isoelectric Point: 4.4313

>AT99980 WP_041984783.1 NZ_CP027720:6429641-6430066 [Pseudomonas chlororaphis subsp. aureofaciens]
MFKYALEIHEEPGTVWLSCEEIPEFHAAGDTVGEALDSVLDALETALSIYVDERRPIPSGNAEAQAGHVLLCLPALTAAK
VALWNAVLDEGTTKAELARRLGIQRPQVDRLLDFLHDSKIENVERALQELGRRISITVEAA

Download         Length: 426 bp

>AT99980 NZ_CP027720:6429641-6430066 [Pseudomonas chlororaphis subsp. aureofaciens]
ATGTTCAAGTACGCATTGGAAATTCACGAAGAGCCCGGCACCGTCTGGCTTTCGTGTGAGGAGATTCCAGAATTTCACGC
TGCCGGCGACACCGTAGGCGAGGCGCTGGATAGTGTTTTGGATGCGCTCGAAACGGCTCTGTCTATCTATGTCGATGAAC
GCCGCCCGATTCCATCTGGTAACGCCGAGGCTCAGGCAGGGCACGTTCTGTTATGCCTTCCAGCCCTCACCGCGGCGAAG
GTTGCACTGTGGAACGCGGTGTTGGATGAAGGCACCACCAAGGCAGAGCTGGCGCGCCGCCTGGGGATTCAGCGGCCGCA
GGTGGACCGCTTGCTGGACTTCCTGCACGACTCGAAAATCGAAAACGTCGAGCGCGCCCTGCAGGAACTCGGTCGGCGGA
TCTCTATCACCGTCGAAGCTGCATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A285GR45

Antitoxin

Source ID Structure
AlphaFold DB A0A554PIE8

References