Detailed information of TA system TA099977

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 2377390..2377994
• Replicon: chromosome
• Accession: NZ_CP027720
• Organism: Pseudomonas chlororaphis subsp. aureofaciens strain DSM 6698

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: C4K13_RS10825
• Protein ID: WP_041988418.1
• Coordinates: 2377827..2377994 (-)
• Length: 56 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A554PQR3
• Locus tag: C4K13_RS10820
• Protein ID: WP_041988420.1
• Coordinates: 2377390..2377791 (-)
• Length: 134 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C4K13_RS10805	2373273..2375162	+	1890	WP_041988426.1	PAS domain S-box protein	-
C4K13_RS10810	2375159..2375794	+	636	WP_023968669.1	response regulator transcription factor	-
C4K13_RS10815	2375888..2376652	+	765	WP_041988421.1	hypothetical protein	-
C4K13_RS10820	2377390..2377791	-	402	WP_041988420.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
C4K13_RS10825	2377827..2377994	-	168	WP_041988418.1	type II toxin-antitoxin system HicA family toxin	Toxin
C4K13_RS10830	2378374..2378655	-	282	WP_041988415.1	hypothetical protein	-
C4K13_RS10835	2379348..2380673	+	1326	WP_041988412.1	hypothetical protein	-
C4K13_RS10840	2380821..2381201	-	381	WP_124355740.1	hypothetical protein	-
C4K13_RS10845	2382062..2382610	-	549	WP_041988409.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2371468..2384632	13164

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (1-54)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-127)

Sequences

Toxin

Protein

• Length: 56 a.a.
• Molecular weight: 6219.35 Da
• Isoelectric Point: 10.6778

>T99977 WP_041988418.1 NZ_CP027720:c2377994-2377827 [Pseudomonas chlororaphis subsp. aureofaciens]
MIKELEAAGWVLERVTGSHHLFKHPYRPETVPVPHPKKDLPRGTVRAIQKLAGLI

Nucleotide

• Length: 168 bp

>T99977 NZ_CP027720:c2377994-2377827 [Pseudomonas chlororaphis subsp. aureofaciens]
TTGATCAAGGAGCTTGAAGCCGCAGGCTGGGTGCTGGAGCGCGTTACCGGCAGCCATCATCTGTTCAAGCATCCCTACCG
GCCGGAGACCGTTCCAGTGCCTCACCCGAAGAAGGATCTGCCACGGGGGACGGTCAGGGCCATCCAGAAACTGGCCGGGC
TGATATAG

Antitoxin

Protein

• Length: 134 a.a.
• Molecular weight: 14609.56 Da
• Isoelectric Point: 4.6017

>AT99977 WP_041988420.1 NZ_CP027720:c2377791-2377390 [Pseudomonas chlororaphis subsp. aureofaciens]
VQYPICIEWGDEHTATGIQIPDIPGAVTAGDTFEAAYSAAIEIAHVMLEELAREGQTIPLPSPTGTHRANPDFEGMGWGM
LDIDITPYLGKTEKVNVTLPGYVIQRIDRFVREHNIKSRSSFLADAAMEKLGR

Nucleotide

• Length: 402 bp

>AT99977 NZ_CP027720:c2377791-2377390 [Pseudomonas chlororaphis subsp. aureofaciens]
GTGCAATATCCCATCTGCATCGAGTGGGGCGACGAGCACACTGCTACCGGCATCCAGATTCCTGACATTCCTGGCGCGGT
CACCGCCGGCGACACCTTCGAGGCCGCGTACAGCGCCGCCATCGAGATCGCCCACGTCATGCTCGAAGAGCTGGCCCGCG
AGGGGCAAACCATTCCCCTGCCCTCGCCCACCGGCACACACCGCGCCAACCCCGACTTCGAGGGGATGGGCTGGGGCATG
CTGGATATCGATATCACGCCTTACCTGGGCAAAACCGAAAAGGTCAATGTGACCCTGCCGGGTTATGTGATTCAGCGGAT
TGATCGCTTTGTGCGGGAGCACAACATCAAGAGCCGGTCGTCTTTCCTGGCGGATGCGGCGATGGAAAAGCTGGGGCGTT
AA

Structures

Antitoxin

• AlphaFold DB: A0A554PQR3