Detailed information of TA system TA099976

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 324844..325514
• Replicon: chromosome
• Accession: NZ_CP027720
• Organism: Pseudomonas chlororaphis subsp. aureofaciens strain DSM 6698

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A554PKP7
• Locus tag: C4K13_RS01425
• Protein ID: WP_041985377.1
• Coordinates: 324844..325272 (-)
• Length: 143 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A3G7DZE9
• Locus tag: C4K13_RS01430
• Protein ID: WP_009046453.1
• Coordinates: 325269..325514 (-)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C4K13_RS01395	320066..320575	-	510	WP_009046447.1	DUF1097 domain-containing protein	-
C4K13_RS01400	320609..321349	-	741	WP_016702555.1	SDR family oxidoreductase	-
C4K13_RS01405	321479..322369	+	891	WP_041985374.1	LysR family transcriptional regulator	-
C4K13_RS01410	322494..322676	+	183	WP_009041685.1	type II toxin-antitoxin system HicA family toxin	-
C4K13_RS01415	322706..323107	+	402	WP_009046450.1	type II toxin-antitoxin system HicB family antitoxin	-
C4K13_RS01420	323677..324714	+	1038	WP_016702559.1	L-glyceraldehyde 3-phosphate reductase	-
C4K13_RS01425	324844..325272	-	429	WP_041985377.1	type II toxin-antitoxin system VapC family toxin	Toxin
C4K13_RS01430	325269..325514	-	246	WP_009046453.1	plasmid stabilization protein	Antitoxin
C4K13_RS01435	325654..326493	-	840	WP_009046454.1	taurine dioxygenase	-
C4K13_RS01440	326729..327568	-	840	WP_009046455.1	taurine ABC transporter permease TauC	-
C4K13_RS01445	327565..328359	-	795	WP_028683844.1	taurine ABC transporter ATP-binding subunit	-
C4K13_RS01450	328455..329432	-	978	WP_009046457.1	taurine ABC transporter substrate-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-124)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 143 a.a.
• Molecular weight: 15897.41 Da
• Isoelectric Point: 5.4927

>T99976 WP_041985377.1 NZ_CP027720:c325272-324844 [Pseudomonas chlororaphis subsp. aureofaciens]
MIVLDTNVLSELMRPQPDTAVLLWIEAQPVEDLYISAMTMAEILHGIARLPHGKRKQTLHASALAMFEEDFIDRIVPFGT
QAALYYTHLVSHRERSGQPINLVDAQIAAICRVHDAAIATRNTKDFTDTGLIVINPWLPLEV

Nucleotide

• Length: 429 bp

>T99976 NZ_CP027720:c325272-324844 [Pseudomonas chlororaphis subsp. aureofaciens]
ATGATAGTACTCGACACAAATGTGTTGTCAGAACTGATGCGCCCTCAACCGGATACGGCGGTATTGCTGTGGATCGAAGC
GCAACCCGTCGAAGACCTCTACATCAGCGCCATGACCATGGCCGAGATTCTCCACGGTATCGCGCGCCTGCCCCACGGCA
AGCGCAAACAGACGCTGCATGCATCGGCGCTGGCTATGTTCGAGGAAGACTTCATCGACCGCATCGTGCCCTTCGGAACC
CAAGCGGCTCTTTACTACACCCATCTGGTCTCGCATCGTGAAAGATCAGGCCAGCCGATCAATCTGGTGGATGCGCAAAT
TGCAGCGATATGCCGGGTCCACGACGCCGCGATCGCCACTCGTAACACCAAGGATTTCACCGACACCGGACTGATCGTTA
TCAACCCCTGGCTGCCGCTCGAGGTCTGA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 9103.27 Da
• Isoelectric Point: 6.2314

>AT99976 WP_009046453.1 NZ_CP027720:c325514-325269 [Pseudomonas chlororaphis subsp. aureofaciens]
MASLTIRNLDDHIKERLRIEAAHNGHSMEEEARLILRRALSRTADSQGLGSRIRARFAETGGVELELPERTEPARLVDLG
E

Nucleotide

• Length: 246 bp

>AT99976 NZ_CP027720:c325514-325269 [Pseudomonas chlororaphis subsp. aureofaciens]
ATGGCCAGCCTTACGATCCGTAATCTGGACGACCACATCAAAGAACGCCTGCGCATCGAGGCCGCTCACAATGGTCATTC
CATGGAAGAAGAAGCCCGATTGATTTTGCGCCGCGCATTGAGCAGGACAGCTGACAGCCAAGGCCTGGGCAGCCGTATCA
GGGCACGCTTTGCCGAGACCGGCGGAGTAGAGCTGGAGCTGCCTGAACGTACCGAACCGGCACGCTTGGTGGACCTGGGC
GAATGA

Structures

Toxin

• AlphaFold DB: A0A554PKP7

Antitoxin

• AlphaFold DB: A0A3G7DZE9