Detailed information of TA system TA099973

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 6443953..6444542
• Replicon: chromosome
• Accession: NZ_CP027719
• Organism: Pseudomonas chlororaphis subsp. aureofaciens strain P2

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: A0A554PHZ9
• Locus tag: C4K14_RS29420
• Protein ID: WP_041987451.1
• Coordinates: 6444243..6444542 (-)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: C4K14_RS29415
• Protein ID: WP_041987449.1
• Coordinates: 6443953..6444246 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C4K14_RS29395	6439494..6440657	-	1164	WP_041987444.1	acyltransferase	-
C4K14_RS29400	6440852..6442042	-	1191	WP_041987447.1	serine hydrolase	-
C4K14_RS29405	6442169..6443056	+	888	WP_009051074.1	LysR family transcriptional regulator	-
C4K14_RS29410	6443428..6443820	+	393	WP_009051075.1	hypothetical protein	-
C4K14_RS29415	6443953..6444246	-	294	WP_041987449.1	putative addiction module antidote protein	Antitoxin
C4K14_RS29420	6444243..6444542	-	300	WP_041987451.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C4K14_RS29425	6444713..6445162	+	450	WP_124314409.1	DUF3828 domain-containing protein	-
C4K14_RS29430	6445187..6446197	-	1011	WP_173424526.1	NAD(P)-dependent alcohol dehydrogenase	-
C4K14_RS29435	6446255..6446554	-	300	WP_009051080.1	ArsR family transcriptional regulator	-
C4K14_RS29440	6446618..6447709	-	1092	WP_009051081.1	nitrite reductase, copper-containing	-
C4K14_RS29445	6447935..6448177	+	243	WP_007924102.1	exodeoxyribonuclease VII small subunit	-
C4K14_RS29450	6448174..6449061	+	888	WP_009051083.1	(2E,6E)-farnesyl diphosphate synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-96)

Antitoxin

• Cro/C1-type helix-turn-helix domain (47-90)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11231.97 Da
• Isoelectric Point: 10.6086

>T99973 WP_041987451.1 NZ_CP027719:c6444542-6444243 [Pseudomonas chlororaphis subsp. aureofaciens]
MIEIKQTATFMEWENRLKDSRARALIAARIFRLANGLAGDVPPVGEGVSELRIHYGPGYRVYFQQLGSQLVILLCGGDKS
RQSRDIELAKQLARRWSRS

Nucleotide

• Length: 300 bp

>T99973 NZ_CP027719:c6444542-6444243 [Pseudomonas chlororaphis subsp. aureofaciens]
ATGATCGAGATCAAACAGACCGCGACCTTTATGGAGTGGGAAAACAGGCTCAAGGATTCCAGAGCCAGGGCGTTGATCGC
TGCGCGGATTTTTCGCCTGGCCAATGGCCTTGCCGGTGACGTGCCACCGGTGGGCGAGGGCGTCAGCGAGTTGCGCATCC
ATTACGGGCCGGGCTATCGGGTTTACTTCCAGCAGCTTGGTTCGCAACTCGTCATCCTGTTATGCGGCGGCGACAAGAGT
CGCCAGAGCCGCGATATCGAACTGGCCAAACAGCTGGCCCGTCGTTGGAGCCGATCATGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10446.94 Da
• Isoelectric Point: 5.6558

>AT99973 WP_041987449.1 NZ_CP027719:c6444246-6443953 [Pseudomonas chlororaphis subsp. aureofaciens]
MNEPLFDYDPATSLRDPQAIALFMADAFETGDAGYIAKALGVVARAKGMTELAHETGLSREQLYRSFSDKGNPTLKTLLA
VMRALGLDMTARVHGAS

Nucleotide

• Length: 294 bp

>AT99973 NZ_CP027719:c6444246-6443953 [Pseudomonas chlororaphis subsp. aureofaciens]
ATGAACGAACCGCTATTCGACTATGACCCCGCCACGTCCCTGCGTGACCCGCAGGCCATTGCCCTGTTCATGGCGGACGC
CTTCGAGACCGGCGATGCCGGCTACATCGCCAAAGCCCTGGGCGTGGTGGCGCGGGCCAAGGGCATGACCGAACTGGCCC
ATGAAACCGGGCTGTCCCGCGAGCAGCTGTACCGCTCGTTCAGTGACAAGGGCAACCCGACCCTGAAGACCCTGCTGGCG
GTGATGCGCGCCCTGGGGCTGGATATGACGGCGCGGGTGCATGGGGCGTCTTGA

Structures

Toxin

• AlphaFold DB: A0A554PHZ9