Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) PumAB/upstrm_HI1419-dnstrm_HI1420
Location 2483675..2484260 Replicon chromosome
Accession NZ_CP027719
Organism Pseudomonas chlororaphis subsp. aureofaciens strain P2

Toxin (Protein)


Gene name PumA Uniprot ID A0A7S5HMS4
Locus tag C4K14_RS11700 Protein ID WP_016705075.1
Coordinates 2483967..2484260 (-) Length 98 a.a.

Antitoxin (Protein)


Gene name PumB Uniprot ID A0A8A9ZIA4
Locus tag C4K14_RS11695 Protein ID WP_009048177.1
Coordinates 2483675..2483965 (-) Length 97 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C4K14_RS11685 2481187..2482569 + 1383 WP_009048175.1 efflux transporter outer membrane subunit -
C4K14_RS11690 2482688..2483275 + 588 WP_028682910.1 hypothetical protein -
C4K14_RS11695 2483675..2483965 - 291 WP_009048177.1 putative addiction module antidote protein Antitoxin
C4K14_RS11700 2483967..2484260 - 294 WP_016705075.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
C4K14_RS11705 2484495..2485889 - 1395 WP_124313189.1 VOC family protein -
C4K14_RS11710 2486154..2487278 + 1125 WP_124313190.1 diguanylate cyclase -
C4K14_RS11715 2487372..2488616 + 1245 WP_124313191.1 M20/M25/M40 family metallo-hydrolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Addiction module killer protein, predicted

(3-97)

Antitoxin

Uncharacterized protein HI1420

(9-91)


Sequences

Toxin        


Download         Length: 98 a.a.        Molecular weight: 10699.27 Da        Isoelectric Point: 10.0959

>T99972 WP_016705075.1 NZ_CP027719:c2484260-2483967 [Pseudomonas chlororaphis subsp. aureofaciens]
MDYEILQTTVFAQWHTTLRDLRARIAIARRIERASAGNLGDVKNLGGGLSEMRVNTGAGYRLYFTVRGNTLIVLLLGGDK
SSQPADICQARSLAKEF

Download         Length: 294 bp

>T99972 NZ_CP027719:c2484260-2483967 [Pseudomonas chlororaphis subsp. aureofaciens]
ATGGATTACGAGATCCTGCAGACGACCGTCTTTGCCCAATGGCACACCACACTACGGGACCTGCGCGCCAGGATAGCCAT
CGCTCGGCGTATCGAGCGGGCGTCGGCGGGCAACCTGGGCGATGTGAAAAACCTCGGCGGCGGTCTCTCGGAAATGCGCG
TGAATACAGGCGCCGGCTACCGCCTCTATTTCACGGTTCGCGGCAATACCCTCATCGTCCTGCTGCTGGGTGGCGACAAG
TCCAGCCAGCCTGCCGATATCTGCCAGGCCCGTTCCCTGGCCAAGGAGTTCTGA

Antitoxin


Download         Length: 97 a.a.        Molecular weight: 10279.84 Da        Isoelectric Point: 8.0096

>AT99972 WP_009048177.1 NZ_CP027719:c2483965-2483675 [Pseudomonas chlororaphis subsp. aureofaciens]
MSDTLVPFDIATLLDSDQAISEYLSQVLADGDTDELIRALGHVARARGMTQIAKTTGQGRASLYKAFAPGAKPRFDTVVK
VMRALGLQLCTTVPRG

Download         Length: 291 bp

>AT99972 NZ_CP027719:c2483965-2483675 [Pseudomonas chlororaphis subsp. aureofaciens]
ATGTCCGACACACTGGTTCCCTTCGACATCGCCACCCTGCTCGACAGCGACCAGGCGATCAGCGAATACCTGTCCCAGGT
CCTGGCCGATGGCGATACCGACGAACTGATCCGCGCCCTGGGCCATGTCGCCAGGGCCCGGGGCATGACGCAGATTGCCA
AGACCACCGGCCAGGGCCGGGCCAGCCTCTACAAGGCTTTTGCCCCGGGGGCCAAGCCGCGCTTCGATACCGTGGTCAAG
GTCATGCGCGCGCTGGGCCTGCAGCTGTGCACCACCGTGCCCCGAGGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7S5HMS4

Antitoxin

Source ID Structure
AlphaFold DB A0A8A9ZIA4

References