Detailed information of TA system TA099971

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 2453997..2454616
• Replicon: chromosome
• Accession: NZ_CP027719
• Organism: Pseudomonas chlororaphis subsp. aureofaciens strain P2

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A3G7DMG3
• Locus tag: C4K14_RS11565
• Protein ID: WP_009048155.1
• Coordinates: 2454434..2454616 (-)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: C4K14_RS11560
• Protein ID: WP_124313176.1
• Coordinates: 2453997..2454398 (-)
• Length: 134 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C4K14_RS11545	2449741..2451630	+	1890	WP_124313174.1	PAS domain S-box protein	-
C4K14_RS11550	2451627..2452262	+	636	WP_023968669.1	response regulator transcription factor	-
C4K14_RS11555	2452356..2453120	+	765	WP_124313175.1	hypothetical protein	-
C4K14_RS11560	2453997..2454398	-	402	WP_124313176.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
C4K14_RS11565	2454434..2454616	-	183	WP_009048155.1	type II toxin-antitoxin system HicA family toxin	Toxin
C4K14_RS11570	2454993..2455274	-	282	WP_124313177.1	hypothetical protein	-
C4K14_RS11575	2455693..2456049	-	357	WP_124313178.1	hypothetical protein	-
C4K14_RS11580	2456034..2456816	-	783	WP_110175430.1	hypothetical protein	-
C4K14_RS11585	2456827..2457372	-	546	WP_124313179.1	PAAR domain-containing protein	-
C4K14_RS11590	2458355..2458828	-	474	WP_124314556.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2453997..2471956	17959

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (3-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-127)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6799.98 Da
• Isoelectric Point: 10.9678

>T99971 WP_009048155.1 NZ_CP027719:c2454616-2454434 [Pseudomonas chlororaphis subsp. aureofaciens]
VQSRQLIKELEAAGWVLERVTGSHHLFKHPYRPETVPVPHPKKDLPRGTVRAIQKLAGLI

Nucleotide

• Length: 183 bp

>T99971 NZ_CP027719:c2454616-2454434 [Pseudomonas chlororaphis subsp. aureofaciens]
GTGCAAAGCAGACAGTTGATCAAGGAGCTTGAAGCCGCAGGCTGGGTGCTGGAGCGCGTTACCGGCAGCCATCATCTGTT
CAAGCATCCCTACCGGCCGGAGACCGTTCCAGTGCCTCACCCGAAGAAGGATCTGCCACGGGGGACGGTCAGGGCCATCC
AGAAACTGGCCGGGCTGATATAG

Antitoxin

Protein

• Length: 134 a.a.
• Molecular weight: 14598.58 Da
• Isoelectric Point: 4.6034

>AT99971 WP_124313176.1 NZ_CP027719:c2454398-2453997 [Pseudomonas chlororaphis subsp. aureofaciens]
VQYPICIEWGDERTATGIQIPDIPGAVTAGDTFEAAYSAAIEIAHVMLEELAREGQAIPLPSPTGTHRANPDFEGMGWGM
LDIDITPYLGKTEKVNVTLPGYVIQRIDRFVREHNIKSRSSFLADAAMEKLGR

Nucleotide

• Length: 402 bp

>AT99971 NZ_CP027719:c2454398-2453997 [Pseudomonas chlororaphis subsp. aureofaciens]
GTGCAATATCCCATCTGCATCGAGTGGGGCGACGAGCGCACTGCTACCGGCATCCAGATTCCTGACATTCCTGGCGCGGT
CACCGCCGGCGACACCTTCGAGGCCGCGTACAGCGCCGCCATCGAGATCGCCCACGTCATGCTCGAAGAGCTGGCCCGTG
AGGGACAAGCCATTCCCCTGCCCTCGCCCACCGGCACACACCGCGCCAACCCCGACTTCGAGGGGATGGGCTGGGGCATG
CTGGATATCGACATCACGCCTTACCTGGGCAAAACCGAGAAGGTCAATGTGACCCTGCCGGGCTACGTGATTCAACGAAT
CGACCGCTTTGTGCGGGAGCACAACATCAAGAGCCGATCGTCTTTTCTGGCAGATGCGGCGATGGAAAAGCTGGGGCGTT
AA

Structures

Toxin

• AlphaFold DB: A0A3G7DMG3