Detailed information of TA system TA099969

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 975376..975914
• Replicon: chromosome
• Accession: NZ_CP027719
• Organism: Pseudomonas chlororaphis subsp. aureofaciens strain P2

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: C4K14_RS04315
• Protein ID: WP_016701683.1
• Coordinates: 975636..975914 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A285H8D4
• Locus tag: C4K14_RS04310
• Protein ID: WP_016701682.1
• Coordinates: 975376..975639 (+)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C4K14_RS04295	971367..973640	-	2274	WP_041988885.1	TonB-dependent siderophore receptor	-
C4K14_RS04300	973846..974526	+	681	WP_016701680.1	Fe2+-dependent dioxygenase	-
C4K14_RS04305	974530..975315	+	786	WP_124312764.1	sel1 repeat family protein	-
C4K14_RS04310	975376..975639	+	264	WP_016701682.1	ribbon-helix-helix domain-containing protein	Antitoxin
C4K14_RS04315	975636..975914	+	279	WP_016701683.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C4K14_RS04320	975989..977152	-	1164	WP_007923836.1	type III PLP-dependent enzyme	-
C4K14_RS04325	977504..978475	-	972	WP_009046955.1	LysR family transcriptional regulator	-
C4K14_RS04330	978610..979293	+	684	WP_009046956.1	type 1 glutamine amidotransferase domain-containing protein	-
C4K14_RS33270	979294..979949	-	656	Protein_835	zinc-binding dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-91)

Antitoxin

• Ribbon-helix-helix protein, CopG (7-44)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10881.51 Da
• Isoelectric Point: 9.6914

>T99969 WP_016701683.1 NZ_CP027719:975636-975914 [Pseudomonas chlororaphis subsp. aureofaciens]
VRELKWTSKALSDLARLFEFLAPVNRSVAARTVQSLTQAPDTLLTNPRIGEQLEEFQPRDVRRLLVGPYEMRYEIRDATL
YILRVWHVREDR

Nucleotide

• Length: 279 bp

>T99969 NZ_CP027719:975636-975914 [Pseudomonas chlororaphis subsp. aureofaciens]
GTGAGGGAATTGAAATGGACGAGCAAGGCGCTGTCGGACCTGGCCCGACTGTTTGAGTTTCTTGCTCCGGTGAACCGTTC
GGTGGCTGCTCGAACGGTGCAATCGCTTACACAGGCCCCGGATACCCTGCTGACCAATCCGCGTATCGGTGAACAGTTGG
AGGAGTTTCAGCCTCGCGATGTTCGGCGTTTATTGGTCGGGCCTTACGAGATGCGTTACGAGATCCGGGATGCGACGCTC
TACATCCTGCGGGTGTGGCATGTACGAGAAGACCGCTAG

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 9741.14 Da
• Isoelectric Point: 5.6088

>AT99969 WP_016701682.1 NZ_CP027719:975376-975639 [Pseudomonas chlororaphis subsp. aureofaciens]
MTATKVLTAHVPLELADKVDQMASRLERSRGWIVKQALVAWVDQEEERSRLTHEALADVDAGRVIDHQAVQAWAESLMSD
KPLPVPK

Nucleotide

• Length: 264 bp

>AT99969 NZ_CP027719:975376-975639 [Pseudomonas chlororaphis subsp. aureofaciens]
ATGACCGCAACTAAAGTGCTGACCGCACATGTGCCGTTGGAGTTGGCCGACAAGGTCGACCAGATGGCCTCCCGGCTGGA
GCGCTCGCGCGGCTGGATCGTCAAGCAGGCTCTGGTGGCTTGGGTCGATCAGGAAGAGGAGCGTAGTCGCCTGACCCATG
AGGCTTTGGCCGATGTGGATGCTGGGCGTGTCATCGATCACCAGGCTGTCCAGGCATGGGCTGAAAGCCTGATGAGTGAC
AAGCCTTTGCCGGTGCCCAAGTGA

Structures

Antitoxin

• AlphaFold DB: A0A285H8D4