Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 324991..325604 Replicon chromosome
Accession NZ_CP027719
Organism Pseudomonas chlororaphis subsp. aureofaciens strain P2

Toxin (Protein)


Gene name hicA Uniprot ID A0A285EYZ4
Locus tag C4K14_RS01430 Protein ID WP_009041685.1
Coordinates 324991..325173 (+) Length 61 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A554PT90
Locus tag C4K14_RS01435 Protein ID WP_009046450.1
Coordinates 325203..325604 (+) Length 134 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C4K14_RS01400 320768..321025 - 258 WP_038583011.1 hypothetical protein -
C4K14_RS01405 321078..321566 - 489 WP_124312631.1 DoxX family protein -
C4K14_RS01410 321640..322535 - 896 Protein_270 SDR family oxidoreductase -
C4K14_RS01415 322564..323073 - 510 WP_009046447.1 DUF1097 domain-containing protein -
C4K14_RS01420 323107..323847 - 741 WP_124312632.1 SDR family oxidoreductase -
C4K14_RS01425 323976..324866 + 891 WP_038630364.1 LysR family transcriptional regulator -
C4K14_RS01430 324991..325173 + 183 WP_009041685.1 type II toxin-antitoxin system HicA family toxin Toxin
C4K14_RS01435 325203..325604 + 402 WP_009046450.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
C4K14_RS01440 326173..327210 + 1038 WP_124312633.1 L-glyceraldehyde 3-phosphate reductase -
C4K14_RS01445 327340..327768 - 429 WP_023970008.1 type II toxin-antitoxin system VapC family toxin -
C4K14_RS01450 327765..328010 - 246 WP_016702561.1 plasmid stabilization protein -
C4K14_RS01455 328150..328989 - 840 WP_101281653.1 taurine dioxygenase -
C4K14_RS01460 329216..330055 - 840 WP_009046455.1 taurine ABC transporter permease TauC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(3-59)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(3-127)


Sequences

Toxin        


Download         Length: 61 a.a.        Molecular weight: 6933.08 Da        Isoelectric Point: 10.4588

>T99967 WP_009041685.1 NZ_CP027719:324991-325173 [Pseudomonas chlororaphis subsp. aureofaciens]
MRSRELIRMIEEDGWYLIAVKGSHHQYKHLHKPGRVTIPHPDADLPRGTIHSILKQAGLK

Download         Length: 183 bp

>T99967 NZ_CP027719:324991-325173 [Pseudomonas chlororaphis subsp. aureofaciens]
ATGCGCAGTCGGGAACTGATCAGGATGATCGAGGAGGACGGGTGGTATCTGATCGCGGTCAAAGGCAGTCATCACCAATA
CAAGCATCTGCACAAGCCGGGACGGGTCACTATCCCTCACCCGGATGCGGATCTGCCCAGGGGCACGATCCACAGCATCT
TGAAACAGGCGGGCCTGAAATGA

Antitoxin


Download         Length: 134 a.a.        Molecular weight: 14596.44 Da        Isoelectric Point: 4.9133

>AT99967 WP_009046450.1 NZ_CP027719:325203-325604 [Pseudomonas chlororaphis subsp. aureofaciens]
MKFPVVLHKDADSGYGVIVPDVPGCFSAGHTAAQAFENVKEALSLHYEGLVADGEPLPQVHEVDVHIDNPDYAGGVWGVI
EFDITPYFGKAVRFNATLPEQLLERIDQTVKRDQRYRSRSGFLAAAALRELSA

Download         Length: 402 bp

>AT99967 NZ_CP027719:325203-325604 [Pseudomonas chlororaphis subsp. aureofaciens]
ATGAAATTCCCGGTCGTGCTGCACAAGGATGCCGACTCCGGATATGGAGTGATCGTCCCCGATGTGCCGGGCTGTTTTTC
CGCCGGTCATACCGCTGCGCAAGCCTTCGAGAATGTGAAAGAGGCCCTTTCGCTGCATTATGAAGGCCTGGTCGCCGATG
GTGAGCCGCTGCCCCAGGTGCATGAGGTCGACGTGCATATCGACAATCCTGATTACGCCGGTGGCGTGTGGGGCGTGATC
GAGTTCGACATCACGCCTTACTTCGGCAAGGCGGTGCGTTTCAATGCGACGTTGCCGGAGCAACTGTTGGAGCGCATCGA
CCAGACGGTGAAGCGCGACCAGCGCTACCGTTCGCGCTCGGGCTTTCTCGCGGCAGCGGCATTGCGCGAACTATCCGCCT
AG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A285EYZ4

Antitoxin

Source ID Structure
AlphaFold DB A0A554PT90

References