Detailed information of TA system TA099964

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 4166136..4166655
• Replicon: chromosome
• Accession: NZ_CP027718
• Organism: Pseudomonas chlororaphis subsp. aurantiaca strain Q16

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: C4K15_RS18455
• Protein ID: WP_081360541.1
• Coordinates: 4166136..4166417 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A1H3UB71
• Locus tag: C4K15_RS18460
• Protein ID: WP_009044432.1
• Coordinates: 4166407..4166655 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C4K15_RS18425	4161586..4161858	+	273	WP_124338632.1	hypothetical protein	-
C4K15_RS18430	4162278..4162468	+	191	Protein_3606	IS4 family transposase	-
C4K15_RS18435	4162465..4162566	-	102	Protein_3607	ATP-binding protein	-
C4K15_RS18440	4162657..4163448	+	792	WP_106697892.1	methyltransferase domain-containing protein	-
C4K15_RS18445	4163763..4164245	+	483	WP_009049637.1	acyl-CoA thioesterase	-
C4K15_RS18450	4164302..4165951	+	1650	WP_124338633.1	FMN-binding glutamate synthase family protein	-
C4K15_RS18455	4166136..4166417	-	282	WP_081360541.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C4K15_RS18460	4166407..4166655	-	249	WP_009044432.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
C4K15_RS18465	4166752..4167540	-	789	WP_124308964.1	helix-turn-helix transcriptional regulator	-
C4K15_RS18470	4167625..4168623	+	999	WP_124308965.1	bile acid:sodium symporter	-
C4K15_RS18475	4168837..4170600	+	1764	WP_124338634.1	hypothetical protein	-
C4K15_RS18480	4170673..4171134	+	462	WP_009049642.1	YccF domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-81)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10821.62 Da
• Isoelectric Point: 10.5816

>T99964 WP_081360541.1 NZ_CP027718:c4166417-4166136 [Pseudomonas chlororaphis subsp. aurantiaca]
MTYELEFSEKAWKEWQKLGPTLKEQFKKKLQERLVNPHVPADRLSGLGNAYKIKLRTAGYRLVYRVVDEVLVVTVIAVGK
RERGSVYQQASKR

Nucleotide

• Length: 282 bp

>T99964 NZ_CP027718:c4166417-4166136 [Pseudomonas chlororaphis subsp. aurantiaca]
ATGACTTATGAGCTGGAGTTCTCCGAGAAAGCCTGGAAGGAATGGCAAAAGCTGGGCCCCACGCTGAAAGAGCAATTCAA
GAAGAAGCTGCAGGAACGACTCGTCAACCCCCATGTCCCGGCCGATCGCCTGAGCGGTTTGGGCAATGCCTACAAAATCA
AACTGCGCACAGCGGGCTACCGCCTGGTGTATCGGGTGGTGGATGAGGTGTTGGTGGTGACGGTCATTGCCGTGGGCAAG
CGAGAGCGGGGGAGTGTTTATCAGCAAGCCAGCAAGCGTTAG

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 8997.37 Da
• Isoelectric Point: 4.4142

>AT99964 WP_009044432.1 NZ_CP027718:c4166655-4166407 [Pseudomonas chlororaphis subsp. aurantiaca]
MTHVLLSNVVASISELKKNPMGTVAAGGGSSVAILNRNEPAFYCVPAKEYEAMMERLEDMELLALCRERENDPTIKVSLD
DL

Nucleotide

• Length: 249 bp

>AT99964 NZ_CP027718:c4166655-4166407 [Pseudomonas chlororaphis subsp. aurantiaca]
ATGACCCATGTCCTTCTGTCCAACGTCGTCGCGAGCATTTCCGAGCTCAAGAAAAACCCCATGGGCACCGTTGCGGCCGG
TGGCGGTTCCTCCGTGGCCATCCTCAATCGCAACGAACCGGCGTTTTACTGCGTTCCGGCCAAGGAATACGAAGCCATGA
TGGAGCGTCTGGAAGATATGGAGCTGCTCGCCTTGTGCCGTGAACGAGAGAATGATCCGACCATCAAGGTTTCGCTCGAT
GACTTATGA

Structures

Antitoxin

• AlphaFold DB: A0A1H3UB71