Detailed information of TA system TA099963

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/upstrm_HI1419-dnstrm_HI1420
• Location: 2373194..2373764
• Replicon: chromosome
• Accession: NZ_CP027718
• Organism: Pseudomonas chlororaphis subsp. aurantiaca strain Q16

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: C4K15_RS10785
• Protein ID: WP_081363241.1
• Coordinates: 2373486..2373764 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: A0A3G7ICX6
• Locus tag: C4K15_RS10780
• Protein ID: WP_038580367.1
• Coordinates: 2373194..2373484 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C4K15_RS10765	2368547..2371027	+	2481	WP_124339346.1	FtsX-like permease family protein	-
C4K15_RS10770	2371017..2372093	+	1077	WP_124337979.1	iron ABC transporter permease	-
C4K15_RS10775	2372207..2372794	+	588	WP_124308120.1	hypothetical protein	-
C4K15_RS10780	2373194..2373484	-	291	WP_038580367.1	putative addiction module antidote protein	Antitoxin
C4K15_RS10785	2373486..2373764	-	279	WP_081363241.1	addiction module antitoxin RelB	Toxin
C4K15_RS10790	2374013..2375407	-	1395	WP_081362128.1	VOC family protein	-
C4K15_RS10795	2375669..2376793	+	1125	WP_124337980.1	diguanylate cyclase	-
C4K15_RS10800	2376887..2378131	+	1245	WP_124337981.1	M20/M25/M40 family metallo-hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (2-92)

Antitoxin

• Uncharacterized protein HI1420 (8-94)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10061.59 Da
• Isoelectric Point: 11.0291

>T99963 WP_081363241.1 NZ_CP027718:c2373764-2373486 [Pseudomonas chlororaphis subsp. aurantiaca]
MQTTVFAQWHTTLRDLRARIAIARRIERASAGNLGDVKNLGGGLSEMRVNTGAGYRLYFTVRGNPLIVLLLGGDKSSQPA
DICQARSLAKEF

Nucleotide

• Length: 279 bp

>T99963 NZ_CP027718:c2373764-2373486 [Pseudomonas chlororaphis subsp. aurantiaca]
CTGCAGACGACCGTCTTTGCCCAATGGCACACCACACTACGGGACCTGCGCGCCAGGATAGCCATCGCTCGGCGTATCGA
GCGGGCTTCGGCGGGCAACCTGGGCGATGTGAAAAACCTCGGCGGCGGTCTCTCGGAAATGCGCGTGAACACAGGCGCTG
GCTACCGCCTCTATTTCACGGTTCGCGGCAATCCCCTCATCGTCCTGCTGCTGGGTGGCGACAAGTCCAGCCAGCCTGCC
GATATCTGCCAGGCCCGTTCCCTGGCCAAGGAGTTCTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10239.78 Da
• Isoelectric Point: 8.0096

>AT99963 WP_038580367.1 NZ_CP027718:c2373484-2373194 [Pseudomonas chlororaphis subsp. aurantiaca]
MSDTLVPFDIAALLDSDQAISEYLSQVLADGDTDELIRALGHVARARGMTQIAKTTGQGRASLYKAFAPGAKPRFDTVVK
VMRALGLQLCTTVSRG

Nucleotide

• Length: 291 bp

>AT99963 NZ_CP027718:c2373484-2373194 [Pseudomonas chlororaphis subsp. aurantiaca]
ATGTCCGACACACTGGTTCCCTTCGACATCGCCGCCCTGCTCGACAGCGACCAGGCGATCAGCGAATACCTGTCCCAGGT
CCTGGCCGATGGCGATACCGACGAACTGATCCGCGCCCTGGGCCACGTCGCCAGGGCCCGGGGCATGACGCAGATTGCCA
AGACCACCGGCCAGGGCCGGGCCAGCCTCTACAAGGCTTTTGCCCCGGGGGCCAAGCCGCGCTTCGATACCGTGGTCAAG
GTCATGCGCGCGCTGGGCCTGCAGCTGTGCACCACCGTGTCCCGAGGCTGA

Structures

Antitoxin

• AlphaFold DB: A0A3G7ICX6