Detailed information of TA system TA099962

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 2359431..2360050
• Replicon: chromosome
• Accession: NZ_CP027718
• Organism: Pseudomonas chlororaphis subsp. aurantiaca strain Q16

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: C4K15_RS10705
• Protein ID: WP_124337971.1
• Coordinates: 2359868..2360050 (-)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A285IZU0
• Locus tag: C4K15_RS10700
• Protein ID: WP_007923747.1
• Coordinates: 2359431..2359832 (-)
• Length: 134 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C4K15_RS10685	2355297..2357186	+	1890	WP_124337970.1	PAS domain S-box protein	-
C4K15_RS10690	2357183..2357818	+	636	WP_023968669.1	response regulator transcription factor	-
C4K15_RS10695	2357911..2358675	+	765	WP_103330830.1	hypothetical protein	-
C4K15_RS10700	2359431..2359832	-	402	WP_007923747.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
C4K15_RS10705	2359868..2360050	-	183	WP_124337971.1	type II toxin-antitoxin system HicA family toxin	Toxin
C4K15_RS10710	2360301..2360804	-	504	WP_124339345.1	zinc ribbon domain-containing protein	-
C4K15_RS10720	2361412..2361693	-	282	WP_124337972.1	hypothetical protein	-
C4K15_RS10725	2362025..2362720	-	696	WP_124337973.1	hypothetical protein	-
C4K15_RS10730	2363081..2363632	-	552	WP_124337974.1	hypothetical protein	-
C4K15_RS10735	2363982..2364875	+	894	WP_124337975.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (3-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-127)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6772.91 Da
• Isoelectric Point: 10.8243

>T99962 WP_124337971.1 NZ_CP027718:c2360050-2359868 [Pseudomonas chlororaphis subsp. aurantiaca]
VQSRQLIKELEAAGWVLERVTGSHHLFTHPYRPETVPVPHPKKDLPRGTVRAIQKLAGLI

Nucleotide

• Length: 183 bp

>T99962 NZ_CP027718:c2360050-2359868 [Pseudomonas chlororaphis subsp. aurantiaca]
GTGCAAAGCAGACAGTTGATCAAGGAGCTTGAAGCCGCAGGCTGGGTGCTGGAGCGCGTTACCGGCAGCCATCATCTGTT
CACGCATCCCTACCGGCCCGAGACCGTTCCGGTGCCTCACCCGAAGAAGGATCTGCCACGGGGGACGGTCAGGGCCATCC
AGAAACTGGCCGGGCTGATATAG

Antitoxin

Protein

• Length: 134 a.a.
• Molecular weight: 14579.54 Da
• Isoelectric Point: 4.6017

>AT99962 WP_007923747.1 NZ_CP027718:c2359832-2359431 [Pseudomonas chlororaphis subsp. aurantiaca]
VQYPICIEWGDEHTATGIQIPDIPGAVTAGDTFEAAYSAAIEIAHVMLEELAREGQAIPLPSPTGTHRANPDFEGMGWGM
LDIDITPYLGKTEKVNVTLPGYVIQRIDRFVREHNIKSRSSFLADAAMEKLGR

Nucleotide

• Length: 402 bp

>AT99962 NZ_CP027718:c2359832-2359431 [Pseudomonas chlororaphis subsp. aurantiaca]
GTGCAATATCCCATCTGCATCGAGTGGGGCGACGAGCACACTGCTACCGGCATCCAGATTCCTGACATTCCTGGCGCGGT
CACCGCCGGCGACACCTTCGAGGCCGCGTACAGCGCCGCCATCGAGATCGCCCACGTCATGCTCGAAGAACTGGCCCGTG
AGGGACAAGCCATTCCCCTGCCCTCGCCCACCGGCACGCACCGCGCCAACCCCGATTTCGAGGGCATGGGCTGGGGCATG
CTGGATATCGATATCACGCCTTACCTGGGCAAGACCGAGAAGGTCAATGTGACCCTGCCGGGCTACGTGATTCAACGAAT
CGACCGCTTTGTACGGGAGCACAACATCAAGAGCCGGTCGTCTTTCCTGGCGGATGCGGCGATGGAAAAGCTGGGGCGTT
AA

Structures

Antitoxin

• AlphaFold DB: A0A285IZU0