Detailed information of TA system TA099958

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 4172320..4172839
• Replicon: chromosome
• Accession: NZ_CP027717
• Organism: Pseudomonas chlororaphis subsp. aurantiaca strain PCM 2210

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: C4K16_RS18570
• Protein ID: WP_124299847.1
• Coordinates: 4172320..4172601 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A1H3UB71
• Locus tag: C4K16_RS18575
• Protein ID: WP_009044432.1
• Coordinates: 4172591..4172839 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C4K16_RS18550	4167905..4168177	+	273	WP_081360544.1	hypothetical protein	-
C4K16_RS18555	4168840..4169631	+	792	WP_124336920.1	methyltransferase domain-containing protein	-
C4K16_RS18560	4169947..4170429	+	483	WP_009049637.1	acyl-CoA thioesterase	-
C4K16_RS18565	4170486..4172135	+	1650	WP_124336921.1	FMN-binding glutamate synthase family protein	-
C4K16_RS18570	4172320..4172601	-	282	WP_124299847.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C4K16_RS18575	4172591..4172839	-	249	WP_009044432.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
C4K16_RS18580	4172926..4173714	-	789	WP_124299848.1	helix-turn-helix transcriptional regulator	-
C4K16_RS18585	4173799..4174797	+	999	WP_124336922.1	bile acid:sodium symporter	-
C4K16_RS18590	4174824..4175186	-	363	WP_124336923.1	hypothetical protein	-
C4K16_RS18595	4175192..4175467	-	276	WP_124336924.1	hypothetical protein	-
C4K16_RS18600	4175783..4177549	+	1767	WP_124336925.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4168840..4177549	8709

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-83)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10793.57 Da
• Isoelectric Point: 10.5816

>T99958 WP_124299847.1 NZ_CP027717:c4172601-4172320 [Pseudomonas chlororaphis subsp. aurantiaca]
MTYELEFSEKAWKEWQKLGPTLKEQFKKKLQERLVNPHVPADRLSGLGNAYKIKLRTAGYRLVYRVVDEVLVVTVIAVGK
RERGSAYQQASKR

Nucleotide

• Length: 282 bp

>T99958 NZ_CP027717:c4172601-4172320 [Pseudomonas chlororaphis subsp. aurantiaca]
ATGACTTATGAGCTGGAGTTCTCCGAGAAAGCCTGGAAGGAATGGCAAAAGCTGGGCCCCACGCTGAAAGAGCAATTCAA
GAAGAAGCTGCAGGAACGACTCGTCAACCCCCATGTCCCGGCCGATCGCCTGAGCGGTTTGGGCAATGCCTACAAAATCA
AACTGCGCACAGCGGGCTACCGCCTGGTGTATCGGGTGGTGGATGAGGTGCTGGTGGTGACGGTCATTGCCGTGGGCAAG
CGAGAGCGGGGGAGTGCCTATCAGCAAGCCAGCAAGCGTTAG

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 8997.37 Da
• Isoelectric Point: 4.4142

>AT99958 WP_009044432.1 NZ_CP027717:c4172839-4172591 [Pseudomonas chlororaphis subsp. aurantiaca]
MTHVLLSNVVASISELKKNPMGTVAAGGGSSVAILNRNEPAFYCVPAKEYEAMMERLEDMELLALCRERENDPTIKVSLD
DL

Nucleotide

• Length: 249 bp

>AT99958 NZ_CP027717:c4172839-4172591 [Pseudomonas chlororaphis subsp. aurantiaca]
ATGACCCATGTCCTTCTGTCCAACGTCGTTGCGAGCATTTCCGAGCTCAAGAAAAACCCCATGGGCACCGTTGCGGCCGG
TGGCGGTTCCTCCGTGGCCATCCTCAATCGCAACGAACCGGCGTTTTACTGCGTTCCGGCCAAGGAATACGAAGCCATGA
TGGAGCGTCTGGAAGATATGGAGCTGCTCGCCTTGTGCCGTGAGCGAGAGAATGATCCGACCATCAAGGTTTCGCTCGAT
GACTTATGA

Structures

Antitoxin

• AlphaFold DB: A0A1H3UB71