Detailed information of TA system TA099957

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/upstrm_HI1419-dnstrm_HI1420
• Location: 2326096..2326666
• Replicon: chromosome
• Accession: NZ_CP027717
• Organism: Pseudomonas chlororaphis subsp. aurantiaca strain PCM 2210

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: C4K16_RS10640
• Protein ID: WP_124337412.1
• Coordinates: 2326388..2326666 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: A0A3G7ICX6
• Locus tag: C4K16_RS10635
• Protein ID: WP_038580367.1
• Coordinates: 2326096..2326386 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C4K16_RS10620	2321413..2323929	+	2517	WP_164485493.1	FtsX-like permease family protein	-
C4K16_RS10625	2323919..2324995	+	1077	WP_124336312.1	iron ABC transporter permease	-
C4K16_RS10630	2325109..2325696	+	588	WP_124336313.1	hypothetical protein	-
C4K16_RS10635	2326096..2326386	-	291	WP_038580367.1	putative addiction module antidote protein	Antitoxin
C4K16_RS10640	2326388..2326666	-	279	WP_124337412.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C4K16_RS10645	2326915..2328309	-	1395	WP_124336314.1	VOC family protein	-
C4K16_RS10650	2328574..2329698	+	1125	WP_124336315.1	diguanylate cyclase	-
C4K16_RS10655	2329792..2331036	+	1245	WP_124336316.1	M20/M25/M40 family metallo-hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2302493..2326759	24266

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (2-92)

Antitoxin

• Uncharacterized protein HI1420 (8-94)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10065.58 Da
• Isoelectric Point: 11.0291

>T99957 WP_124337412.1 NZ_CP027717:c2326666-2326388 [Pseudomonas chlororaphis subsp. aurantiaca]
MQTTVFAQWHTTLRDLRARIAIARRIERASAGNLGDVKNLGGGLSEMRVNTGAGYRLYFTVRGNTLIVLLLGGDKSSQPA
DICQARSLAKEF

Nucleotide

• Length: 279 bp

>T99957 NZ_CP027717:c2326666-2326388 [Pseudomonas chlororaphis subsp. aurantiaca]
CTGCAGACGACCGTCTTTGCCCAATGGCACACCACACTACGGGACCTGCGCGCCAGGATAGCCATCGCTCGGCGTATCGA
GCGGGCTTCGGCGGGCAACCTGGGCGATGTGAAAAACCTCGGCGGCGGTCTCTCGGAAATGCGCGTGAACACAGGCGCCG
GCTACCGCCTCTATTTCACGGTTCGCGGCAATACCCTCATCGTCCTGCTGCTGGGTGGCGACAAGTCCAGCCAGCCTGCC
GATATCTGCCAGGCCCGTTCCCTGGCCAAGGAGTTCTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10239.78 Da
• Isoelectric Point: 8.0096

>AT99957 WP_038580367.1 NZ_CP027717:c2326386-2326096 [Pseudomonas chlororaphis subsp. aurantiaca]
MSDTLVPFDIAALLDSDQAISEYLSQVLADGDTDELIRALGHVARARGMTQIAKTTGQGRASLYKAFAPGAKPRFDTVVK
VMRALGLQLCTTVSRG

Nucleotide

• Length: 291 bp

>AT99957 NZ_CP027717:c2326386-2326096 [Pseudomonas chlororaphis subsp. aurantiaca]
ATGTCCGACACACTGGTTCCCTTCGACATCGCCGCCCTGCTCGACAGCGACCAGGCGATCAGCGAATACCTGTCCCAGGT
CCTGGCCGATGGCGATACCGACGAACTGATCCGCGCCCTGGGCCACGTCGCCAGGGCCCGGGGCATGACGCAGATTGCCA
AGACCACCGGCCAGGGCCGGGCCAGCCTCTACAAGGCTTTTGCCCCGGGGGCCAAGCCGCGCTTCGATACCGTGGTCAAG
GTCATGCGCGCGCTGGGCCTGCAGCTGTGCACCACCGTGTCCCGAGGCTGA

Structures

Antitoxin

• AlphaFold DB: A0A3G7ICX6