Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 326032..326645 Replicon chromosome
Accession NZ_CP027717
Organism Pseudomonas chlororaphis subsp. aurantiaca strain PCM 2210

Toxin (Protein)


Gene name hicA Uniprot ID A0A285EYZ4
Locus tag C4K16_RS01430 Protein ID WP_009041685.1
Coordinates 326032..326214 (+) Length 61 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A554PT90
Locus tag C4K16_RS01435 Protein ID WP_009046450.1
Coordinates 326244..326645 (+) Length 134 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C4K16_RS01400 321799..322056 - 258 WP_038583011.1 hypothetical protein -
C4K16_RS01405 322109..322597 - 489 WP_038583009.1 DoxX family protein -
C4K16_RS01410 322671..323566 - 896 Protein_270 SDR family oxidoreductase -
C4K16_RS01415 323595..324104 - 510 WP_124333844.1 DUF1097 domain-containing protein -
C4K16_RS01420 324138..324878 - 741 WP_124335933.1 SDR family oxidoreductase -
C4K16_RS01425 325017..325907 + 891 WP_124335934.1 LysR family transcriptional regulator -
C4K16_RS01430 326032..326214 + 183 WP_009041685.1 type II toxin-antitoxin system HicA family toxin Toxin
C4K16_RS01435 326244..326645 + 402 WP_009046450.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
C4K16_RS01440 326745..327791 - 1047 WP_081362983.1 LacI family DNA-binding transcriptional regulator -
C4K16_RS01445 327999..330257 + 2259 WP_124335935.1 TonB-dependent receptor -
C4K16_RS01450 330304..331629 + 1326 WP_081362981.1 LLM class flavin-dependent oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(3-59)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(3-127)


Sequences

Toxin        


Download         Length: 61 a.a.        Molecular weight: 6933.08 Da        Isoelectric Point: 10.4588

>T99955 WP_009041685.1 NZ_CP027717:326032-326214 [Pseudomonas chlororaphis subsp. aurantiaca]
MRSRELIRMIEEDGWYLIAVKGSHHQYKHLHKPGRVTIPHPDADLPRGTIHSILKQAGLK

Download         Length: 183 bp

>T99955 NZ_CP027717:326032-326214 [Pseudomonas chlororaphis subsp. aurantiaca]
ATGCGCAGTCGGGAACTGATCAGGATGATCGAGGAGGACGGGTGGTATCTGATCGCAGTCAAAGGCAGTCATCACCAGTA
CAAGCATCTGCACAAGCCGGGGCGGGTCACTATCCCTCACCCGGATGCGGATCTGCCCAGGGGCACGATCCACAGCATCT
TGAAACAGGCGGGCCTGAAATGA

Antitoxin


Download         Length: 134 a.a.        Molecular weight: 14596.44 Da        Isoelectric Point: 4.9133

>AT99955 WP_009046450.1 NZ_CP027717:326244-326645 [Pseudomonas chlororaphis subsp. aurantiaca]
MKFPVVLHKDADSGYGVIVPDVPGCFSAGHTAAQAFENVKEALSLHYEGLVADGEPLPQVHEVDVHIDNPDYAGGVWGVI
EFDITPYFGKAVRFNATLPEQLLERIDQTVKRDQRYRSRSGFLAAAALRELSA

Download         Length: 402 bp

>AT99955 NZ_CP027717:326244-326645 [Pseudomonas chlororaphis subsp. aurantiaca]
ATGAAATTCCCGGTCGTGCTGCACAAGGATGCCGACTCCGGATATGGAGTGATCGTCCCCGATGTGCCGGGCTGTTTTTC
CGCCGGTCATACCGCTGCGCAAGCCTTCGAGAATGTGAAAGAGGCCCTGTCGCTGCACTACGAAGGCCTGGTCGCCGATG
GTGAGCCGTTGCCCCAGGTGCATGAAGTCGACGTGCATATCGACAATCCCGATTACGCCGGTGGCGTGTGGGGGGTGATC
GAGTTCGACATCACGCCTTACTTCGGCAAGGCGGTGCGTTTCAATGCGACGTTGCCGGAGCAACTGTTGGAGCGCATCGA
CCAGACGGTGAAGCGCGACCAGCGCTACCGTTCGCGCTCGGGTTTTCTCGCGGCAGCGGCATTGCGCGAGTTGTCGGCGT
AG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A285EYZ4

Antitoxin

Source ID Structure
AlphaFold DB A0A554PT90

References