Detailed information of TA system TA099954

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): stbE-relB/ParE-YafN
• Location: 213595..214111
• Replicon: chromosome
• Accession: NZ_CP027717
• Organism: Pseudomonas chlororaphis subsp. aurantiaca strain PCM 2210

Toxin (Protein)

• Gene name: stbE
• Uniprot ID: A0A8A9Z863
• Locus tag: C4K16_RS00895
• Protein ID: WP_028681510.1
• Coordinates: 213830..214111 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: C4K16_RS00890
• Protein ID: WP_023969916.1
• Coordinates: 213595..213840 (+)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C4K16_RS00865	209363..210358	+	996	WP_124335894.1	sulfotransferase family protein	-
C4K16_RS00870	210324..211082	-	759	WP_038583164.1	slipin family protein	-
C4K16_RS00875	211084..212469	-	1386	WP_081363045.1	nodulation protein NfeD	-
C4K16_RS00880	212693..213154	+	462	WP_016705004.1	YbaK/EbsC family protein	-
C4K16_RS00885	213285..213530	+	246	WP_023969915.1	DUF2789 domain-containing protein	-
C4K16_RS00890	213595..213840	+	246	WP_023969916.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
C4K16_RS00895	213830..214111	+	282	WP_028681510.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C4K16_RS00900	214244..214681	+	438	WP_038583159.1	winged helix DNA-binding protein	-
C4K16_RS00905	214678..216762	+	2085	WP_124335895.1	FUSC family protein	-
C4K16_RS00910	216759..216968	+	210	WP_007932080.1	DUF1656 domain-containing protein	-
C4K16_RS00915	216965..217861	+	897	WP_016705006.1	HlyD family secretion protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-83)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (8-70)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10907.82 Da
• Isoelectric Point: 10.6258

>T99954 WP_028681510.1 NZ_CP027717:213830-214111 [Pseudomonas chlororaphis subsp. aurantiaca]
MTYKLQFLPSARKEWDKLGHTLREQFKRKLAERLEMPRVPADALHGMADCYKIKLKASGYRLVYQVIEDQVVVSVVAVGK
RERSAVYERARKR

Nucleotide

• Length: 282 bp

>T99954 NZ_CP027717:213830-214111 [Pseudomonas chlororaphis subsp. aurantiaca]
ATGACTTATAAGCTCCAGTTTCTGCCTTCGGCGCGCAAGGAATGGGACAAGCTCGGTCATACCCTGCGCGAGCAATTCAA
GAGGAAGCTGGCCGAGCGCCTGGAAATGCCACGGGTGCCCGCGGACGCCCTTCACGGTATGGCGGACTGCTACAAGATCA
AGCTCAAGGCCTCTGGCTATCGCCTGGTTTACCAAGTGATCGAGGATCAGGTCGTGGTTTCGGTGGTGGCCGTGGGCAAA
CGTGAACGCAGTGCCGTGTATGAGCGGGCACGAAAGCGCTGA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 8840.18 Da
• Isoelectric Point: 4.7164

>AT99954 WP_023969916.1 NZ_CP027717:213595-213840 [Pseudomonas chlororaphis subsp. aurantiaca]
MQSVLADKAVSVSELKKNPSAVLNGAQGGPVAVLNHNRVMGYMVPADVFEAMMERLDDLELAEIVRARRHETPVPVNLDD
L

Nucleotide

• Length: 246 bp

>AT99954 NZ_CP027717:213595-213840 [Pseudomonas chlororaphis subsp. aurantiaca]
ATGCAAAGCGTATTGGCTGATAAAGCTGTCAGCGTGTCTGAACTGAAAAAAAATCCTTCGGCTGTCCTGAACGGCGCACA
GGGTGGTCCGGTTGCGGTGCTCAATCACAACCGGGTCATGGGCTATATGGTCCCGGCCGACGTGTTTGAAGCCATGATGG
AGCGTCTTGACGATCTTGAACTGGCGGAGATTGTCCGTGCCCGTCGTCACGAGACCCCGGTCCCGGTAAATCTGGATGAC
TTATAA

Structures

Toxin

• AlphaFold DB: A0A8A9Z863