Detailed information of TA system TA099951

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/upstrm_HI1419-dnstrm_HI1420
• Location: 2288296..2288881
• Replicon: chromosome
• Accession: NZ_CP027716
• Organism: Pseudomonas chlororaphis strain Pb-St2

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: A0A7S5HMS4
• Locus tag: C4K23_RS10545
• Protein ID: WP_016705075.1
• Coordinates: 2288588..2288881 (-)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: C4K23_RS10540
• Protein ID: WP_023968688.1
• Coordinates: 2288296..2288586 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C4K23_RS10530	2285808..2287190	+	1383	WP_023968686.1	efflux transporter outer membrane subunit	-
C4K23_RS10535	2287309..2287896	+	588	WP_023968687.1	hypothetical protein	-
C4K23_RS10540	2288296..2288586	-	291	WP_023968688.1	putative addiction module antidote protein	Antitoxin
C4K23_RS10545	2288588..2288881	-	294	WP_016705075.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C4K23_RS10550	2289117..2290511	-	1395	WP_023968689.1	VOC family protein	-
C4K23_RS10555	2290775..2291899	+	1125	WP_051408723.1	diguanylate cyclase	-
C4K23_RS10560	2291993..2293237	+	1245	WP_023968691.1	M20/M25/M40 family metallo-hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-97)

Antitoxin

• Uncharacterized protein HI1420 (5-91)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 10699.27 Da
• Isoelectric Point: 10.0959

>T99951 WP_016705075.1 NZ_CP027716:c2288881-2288588 [Pseudomonas chlororaphis]
MDYEILQTTVFAQWHTTLRDLRARIAIARRIERASAGNLGDVKNLGGGLSEMRVNTGAGYRLYFTVRGNTLIVLLLGGDK
SSQPADICQARSLAKEF

Nucleotide

• Length: 294 bp

>T99951 NZ_CP027716:c2288881-2288588 [Pseudomonas chlororaphis]
ATGGATTACGAGATCCTGCAGACGACCGTCTTTGCCCAATGGCACACCACCCTACGGGACCTGCGCGCCAGGATAGCCAT
CGCTCGGCGTATCGAGCGGGCTTCGGCGGGCAACCTGGGCGATGTGAAAAACCTCGGCGGCGGTCTCTCGGAAATGCGCG
TGAACACAGGCGCCGGCTACCGCCTCTATTTCACGGTTCGCGGCAATACCCTCATCGTCCTGCTGCTGGGTGGCGACAAG
TCCAGCCAGCCTGCCGATATCTGCCAGGCCCGTTCCCTGGCCAAGGAGTTCTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10223.78 Da
• Isoelectric Point: 8.0096

>AT99951 WP_023968688.1 NZ_CP027716:c2288586-2288296 [Pseudomonas chlororaphis]
MSDTLLPFDIAALLDSDQAISEYLSQVLADGDTDELIRALGHVARARGMTQIAKTTGQGRASLYKAFAPGAKPRFDTVVK
VMRALGLQLCATVSRG

Nucleotide

• Length: 291 bp

>AT99951 NZ_CP027716:c2288586-2288296 [Pseudomonas chlororaphis]
ATGTCCGACACACTGCTTCCCTTCGACATCGCCGCCCTGCTCGACAGCGACCAGGCGATCAGCGAATACCTGTCCCAGGT
CCTGGCCGATGGCGATACCGATGAACTGATCCGCGCCCTGGGCCACGTCGCCAGGGCCCGGGGCATGACGCAGATTGCCA
AGACCACCGGCCAGGGCCGGGCCAGCCTCTACAAGGCTTTTGCCCCGGGGGCCAAGCCGCGCTTCGATACCGTGGTCAAG
GTCATGCGCGCGCTGGGCCTGCAGCTGTGCGCCACCGTGTCCCGAGGCTGA

Structures

Toxin

• AlphaFold DB: A0A7S5HMS4