Detailed information of TA system TA099950

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 310340..311010
• Replicon: chromosome
• Accession: NZ_CP027716
• Organism: Pseudomonas chlororaphis strain Pb-St2

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A8A9Z8F6
• Locus tag: C4K23_RS01370
• Protein ID: WP_023970008.1
• Coordinates: 310340..310768 (-)
• Length: 143 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: C4K23_RS01375
• Protein ID: WP_023970009.1
• Coordinates: 310765..311010 (-)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C4K23_RS01340	305565..306053	-	489	WP_023970004.1	DoxX family protein	-
C4K23_RS01345	306092..306832	-	741	WP_023970005.1	SDR family oxidoreductase	-
C4K23_RS01350	306972..307862	+	891	WP_023970006.1	LysR family transcriptional regulator	-
C4K23_RS01355	307987..308168	+	182	Protein_263	type II toxin-antitoxin system HicA family toxin	-
C4K23_RS01360	308198..308599	+	402	WP_009046450.1	type II toxin-antitoxin system HicB family antitoxin	-
C4K23_RS01365	309173..310210	+	1038	WP_023970007.1	L-glyceraldehyde 3-phosphate reductase	-
C4K23_RS01370	310340..310768	-	429	WP_023970008.1	type II toxin-antitoxin system VapC family toxin	Toxin
C4K23_RS01375	310765..311010	-	246	WP_023970009.1	plasmid stabilization protein	Antitoxin
C4K23_RS01380	311150..311989	-	840	WP_023970010.1	taurine dioxygenase	-
C4K23_RS01385	312074..312913	-	840	WP_023970011.1	taurine ABC transporter permease TauC	-
C4K23_RS01390	312910..313704	-	795	WP_023970012.1	taurine ABC transporter ATP-binding subunit	-
C4K23_RS01395	313799..314776	-	978	WP_023970013.1	taurine ABC transporter substrate-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-124)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 143 a.a.
• Molecular weight: 15853.36 Da
• Isoelectric Point: 5.4927

>T99950 WP_023970008.1 NZ_CP027716:c310768-310340 [Pseudomonas chlororaphis]
MIVLDTNVLSELMRPQPDASVLLWIEAQPVEDLYISAMTMAEILHGIARLPHGKRKQTLHASALAMFEEDFIDRIVPFGT
QAALYYAHLVSHRERSGQPINLVDAQIAAICRVHDAAIATRNTKDFTDTGLIVINPWLPLEV

Nucleotide

• Length: 429 bp

>T99950 NZ_CP027716:c310768-310340 [Pseudomonas chlororaphis]
ATGATAGTGCTCGATACCAATGTGCTGTCGGAGCTGATGCGTCCTCAGCCAGACGCCTCGGTGTTGTTGTGGATCGAAGC
GCAACCCGTCGAGGACCTCTACATCAGCGCCATGACCATGGCCGAGATTCTCCACGGTATCGCGCGCCTGCCCCACGGCA
AGCGCAAACAGACGCTGCATGCATCGGCACTGGCCATGTTCGAGGAAGACTTCATCGACCGGATCGTGCCCTTCGGAACC
CAGGCGGCTCTTTATTACGCGCATCTGGTCTCGCATCGCGAAAGATCAGGTCAGCCGATCAACCTGGTGGACGCGCAGAT
TGCAGCGATATGCCGAGTCCATGACGCCGCGATTGCCACCCGTAACACCAAGGACTTCACCGACACCGGGCTGATCGTTA
TCAACCCCTGGCTGCCGCTCGAGGTCTGA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 9084.27 Da
• Isoelectric Point: 6.7287

>AT99950 WP_023970009.1 NZ_CP027716:c311010-310765 [Pseudomonas chlororaphis]
MASLTIRNLDDHIKERLRIEAAHNGHSMEEEARLILRRALSRTAASQGLGSRIRARFAEVGAVELELPERTEPPRAADLN
E

Nucleotide

• Length: 246 bp

>AT99950 NZ_CP027716:c311010-310765 [Pseudomonas chlororaphis]
ATGGCCAGCCTTACGATCCGCAATCTGGACGACCACATCAAAGAACGCCTGCGCATCGAGGCCGCTCACAATGGTCATTC
CATGGAAGAAGAAGCCCGATTGATTTTGCGCCGCGCCCTGAGCAGGACAGCCGCCAGCCAGGGCCTGGGCAGCCGTATCC
GGGCACGCTTTGCCGAGGTCGGCGCAGTGGAACTGGAACTTCCCGAGCGCACCGAGCCCCCGCGCGCAGCGGACCTGAAC
GAATGA

Structures

Toxin

• AlphaFold DB: A0A8A9Z8F6