Detailed information of TA system TA099948

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 5443266..5443894
• Replicon: chromosome
• Accession: NZ_CP027715
• Organism: Pseudomonas chlororaphis subsp. aurantiaca strain M12

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: C4K24_RS24480
• Protein ID: WP_038576104.1
• Coordinates: 5443266..5443664 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: C4K24_RS24485
• Protein ID: WP_038576101.1
• Coordinates: 5443664..5443894 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C4K24_RS24455	5438652..5439149	+	498	WP_081363157.1	PAAR domain-containing protein	-
C4K24_RS24460	5439153..5439887	+	735	WP_124309470.1	alpha/beta hydrolase	-
C4K24_RS24465	5440058..5440414	+	357	WP_080748359.1	hypothetical protein	-
C4K24_RS24470	5440601..5441125	+	525	WP_039968197.1	hypothetical protein	-
C4K24_RS24475	5441259..5443208	+	1950	WP_124309471.1	alkaline phosphatase family protein	-
C4K24_RS24480	5443266..5443664	-	399	WP_038576104.1	tRNA(fMet)-specific endonuclease VapC	Toxin
C4K24_RS24485	5443664..5443894	-	231	WP_038576101.1	antitoxin	Antitoxin
C4K24_RS24490	5444172..5445149	+	978	WP_038576099.1	type I-F CRISPR-associated endonuclease Cas1	-
C4K24_RS24495	5445146..5448565	+	3420	WP_124309472.1	type I-F CRISPR-associated helicase Cas3	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-124)

Antitoxin

• SpoVT-AbrB domain (7-53)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14886.17 Da
• Isoelectric Point: 7.2433

>T99948 WP_038576104.1 NZ_CP027715:c5443664-5443266 [Pseudomonas chlororaphis subsp. aurantiaca]
MLKYMLDTNICIFTIKNKPERVREAFNRQHGRLCISSVTLMELIYGAEKSAAPERNLSVIEGFVARLEVLAYDYEAAIHS
GQLRAELARAGTPIGPYDQLIAGHARSQGLVLVTNNVREFERVPGLRIEDWL

Nucleotide

• Length: 399 bp

>T99948 NZ_CP027715:c5443664-5443266 [Pseudomonas chlororaphis subsp. aurantiaca]
ATGCTCAAGTACATGTTGGATACGAACATCTGTATTTTCACCATCAAGAACAAACCGGAGCGGGTTCGGGAGGCGTTCAA
CCGCCAGCACGGCCGGTTGTGCATCAGTTCGGTGACCTTGATGGAACTGATTTATGGAGCAGAAAAATCAGCGGCTCCTG
AGAGAAACCTGTCTGTCATAGAGGGCTTCGTGGCACGTCTGGAAGTGCTCGCGTATGACTACGAAGCTGCTATTCACAGC
GGTCAGTTGCGTGCCGAGTTGGCCAGGGCTGGTACGCCAATAGGTCCTTACGATCAGCTGATTGCCGGCCATGCCAGATC
CCAGGGATTGGTATTGGTGACCAACAATGTGCGCGAGTTTGAACGGGTGCCAGGGCTGCGTATTGAGGATTGGCTCTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8487.41 Da
• Isoelectric Point: 4.2821

>AT99948 WP_038576101.1 NZ_CP027715:c5443894-5443664 [Pseudomonas chlororaphis subsp. aurantiaca]
MEQGSVFKSNRSQAIRMPKAVALPDDVTRVDIVAVGRTRIITPAGEAWDSWFDGESVSADFMSERDQPAEQEREAF

Nucleotide

• Length: 231 bp

>AT99948 NZ_CP027715:c5443894-5443664 [Pseudomonas chlororaphis subsp. aurantiaca]
ATGGAACAAGGGTCGGTATTCAAAAGTAACCGCAGCCAGGCCATTCGAATGCCCAAGGCAGTGGCTCTCCCTGACGATGT
AACGCGAGTGGATATCGTTGCGGTAGGGCGTACCCGGATCATTACCCCGGCGGGGGAAGCCTGGGACAGCTGGTTCGATG
GCGAGTCGGTGAGCGCGGACTTCATGAGTGAGCGGGATCAGCCTGCGGAACAAGAGCGTGAGGCGTTCTGA