Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) PumAB/upstrm_HI1419-dnstrm_HI1420
Location 2320904..2321489 Replicon chromosome
Accession NZ_CP027715
Organism Pseudomonas chlororaphis subsp. aurantiaca strain M12

Toxin (Protein)


Gene name PumA Uniprot ID A0A7S5HMS4
Locus tag C4K24_RS10650 Protein ID WP_016705075.1
Coordinates 2321196..2321489 (-) Length 98 a.a.

Antitoxin (Protein)


Gene name PumB Uniprot ID A0A3G7ICX6
Locus tag C4K24_RS10645 Protein ID WP_038580367.1
Coordinates 2320904..2321194 (-) Length 97 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C4K24_RS10630 2316208..2318724 + 2517 WP_206428058.1 FtsX-like permease family protein -
C4K24_RS10635 2318714..2319802 + 1089 WP_124308119.1 iron ABC transporter permease -
C4K24_RS10640 2319916..2320503 + 588 WP_124308120.1 hypothetical protein -
C4K24_RS10645 2320904..2321194 - 291 WP_038580367.1 putative addiction module antidote protein Antitoxin
C4K24_RS10650 2321196..2321489 - 294 WP_016705075.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
C4K24_RS10655 2321724..2323118 - 1395 WP_124308121.1 VOC family protein -
C4K24_RS10660 2323383..2324507 + 1125 WP_096373680.1 diguanylate cyclase -
C4K24_RS10665 2324601..2325845 + 1245 WP_124308122.1 M20/M25/M40 family metallo-hydrolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Addiction module killer protein, predicted

(3-97)

Antitoxin

Uncharacterized protein HI1420

(8-94)


Sequences

Toxin        


Download         Length: 98 a.a.        Molecular weight: 10699.27 Da        Isoelectric Point: 10.0959

>T99947 WP_016705075.1 NZ_CP027715:c2321489-2321196 [Pseudomonas chlororaphis subsp. aurantiaca]
MDYEILQTTVFAQWHTTLRDLRARIAIARRIERASAGNLGDVKNLGGGLSEMRVNTGAGYRLYFTVRGNTLIVLLLGGDK
SSQPADICQARSLAKEF

Download         Length: 294 bp

>T99947 NZ_CP027715:c2321489-2321196 [Pseudomonas chlororaphis subsp. aurantiaca]
ATGGATTACGAGATCCTGCAGACGACCGTCTTTGCCCAATGGCACACCACACTACGGGACCTGCGCGCCAGGATAGCCAT
CGCTCGGCGTATCGAGCGGGCTTCGGCGGGCAACCTGGGCGATGTGAAAAACCTCGGCGGCGGTCTCTCGGAAATGCGCG
TGAACACAGGCGCCGGCTACCGCCTCTATTTCACGGTTCGCGGCAATACCCTCATCGTCCTGCTGCTGGGTGGCGACAAG
TCCAGCCAACCTGCCGATATCTGCCAGGCCCGTTCCCTGGCCAAGGAGTTCTGA

Antitoxin


Download         Length: 97 a.a.        Molecular weight: 10239.78 Da        Isoelectric Point: 8.0096

>AT99947 WP_038580367.1 NZ_CP027715:c2321194-2320904 [Pseudomonas chlororaphis subsp. aurantiaca]
MSDTLVPFDIAALLDSDQAISEYLSQVLADGDTDELIRALGHVARARGMTQIAKTTGQGRASLYKAFAPGAKPRFDTVVK
VMRALGLQLCTTVSRG

Download         Length: 291 bp

>AT99947 NZ_CP027715:c2321194-2320904 [Pseudomonas chlororaphis subsp. aurantiaca]
ATGTCCGACACACTGGTTCCCTTCGACATCGCCGCCCTGCTCGACAGCGACCAGGCGATCAGCGAATACCTGTCCCAGGT
CCTGGCCGATGGCGATACCGACGAACTGATCCGCGCCCTGGGGCACGTCGCCAGGGCCCGGGGCATGACGCAGATTGCCA
AGACCACCGGCCAGGGCCGGGCCAGCCTCTACAAGGCTTTTGCCCCGGGGGCCAAGCCGCGCTTCGATACCGTGGTCAAG
GTCATGCGCGCGCTGGGCCTGCAGCTGTGCACCACTGTGTCCCGAGGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7S5HMS4

Antitoxin

Source ID Structure
AlphaFold DB A0A3G7ICX6

References