Detailed information of TA system TA099945

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 1533098..1533720
• Replicon: chromosome
• Accession: NZ_CP027715
• Organism: Pseudomonas chlororaphis subsp. aurantiaca strain M12

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A2N8AR08
• Locus tag: C4K24_RS06975
• Protein ID: WP_007929414.1
• Coordinates: 1533538..1533720 (-)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: C4K24_RS06970
• Protein ID: WP_124307846.1
• Coordinates: 1533098..1533505 (-)
• Length: 136 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C4K24_RS06950	1528410..1529300	+	891	WP_124309894.1	LysR family transcriptional regulator	-
C4K24_RS06955	1529535..1531313	+	1779	WP_124307843.1	GGDEF and EAL domain-containing protein	-
C4K24_RS06960	1531347..1532261	-	915	WP_124307844.1	SGNH/GDSL hydrolase family protein	-
C4K24_RS06965	1532391..1532858	-	468	WP_124307845.1	GAF domain-containing protein	-
C4K24_RS06970	1533098..1533505	-	408	WP_124307846.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
C4K24_RS06975	1533538..1533720	-	183	WP_007929414.1	type II toxin-antitoxin system HicA family toxin	Toxin
C4K24_RS06985	1534435..1535319	-	885	WP_124307847.1	alpha/beta hydrolase	-
C4K24_RS06990	1535671..1536363	-	693	WP_124307848.1	16S rRNA pseudouridine(516) synthase	-
C4K24_RS06995	1536397..1536630	-	234	WP_016702123.1	cysteine-rich CWC family protein	-
C4K24_RS07000	1536623..1538110	-	1488	WP_124307849.1	sensor domain-containing diguanylate cyclase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (6-57)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-128)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6850.15 Da
• Isoelectric Point: 11.6709

>T99945 WP_007929414.1 NZ_CP027715:c1533720-1533538 [Pseudomonas chlororaphis subsp. aurantiaca]
VNSRYLIGQIVADGWYLVRVRGSHHHFRHPSKPGLVTVPHPKKDLLRKTAISILKQALLM

Nucleotide

• Length: 183 bp

>T99945 NZ_CP027715:c1533720-1533538 [Pseudomonas chlororaphis subsp. aurantiaca]
GTGAACAGTCGGTATTTGATCGGCCAAATCGTCGCAGACGGTTGGTATCTGGTCAGGGTCAGAGGCAGTCACCACCATTT
CAGGCACCCCAGTAAACCGGGGCTGGTGACAGTTCCACATCCCAAAAAGGACCTGCTGAGAAAAACCGCCATCAGCATTC
TGAAACAGGCGCTGCTCATGTGA

Antitoxin

Protein

• Length: 136 a.a.
• Molecular weight: 14634.62 Da
• Isoelectric Point: 4.3638

>AT99945 WP_124307846.1 NZ_CP027715:c1533505-1533098 [Pseudomonas chlororaphis subsp. aurantiaca]
MLYPIAISMGDDEHAWGVEVPDIPGCFSAGDDLDDAMAMTREAIEGHFEILAEDGAAIPPANKVTLHAANPQYAGCTWAL
IDIDVTKYLGKAQKLNITLPGYLLNRIDEYVLHHPEAKSRSGFLASAALKVLQQD

Nucleotide

• Length: 408 bp

>AT99945 NZ_CP027715:c1533505-1533098 [Pseudomonas chlororaphis subsp. aurantiaca]
ATGCTTTACCCGATTGCAATTTCAATGGGTGATGACGAACACGCCTGGGGCGTGGAAGTCCCCGACATTCCTGGCTGTTT
CTCGGCCGGCGACGACCTCGACGACGCCATGGCCATGACCCGCGAAGCCATCGAAGGGCACTTCGAGATTCTCGCCGAAG
ACGGTGCCGCCATCCCGCCCGCCAACAAGGTCACCCTGCACGCGGCCAACCCTCAGTACGCAGGCTGCACCTGGGCGCTG
ATCGACATCGATGTCACCAAGTACCTGGGCAAGGCGCAGAAGCTCAACATCACCCTGCCCGGCTACCTGCTGAACCGCAT
CGACGAATACGTGCTGCATCACCCGGAAGCGAAGAGCCGCTCGGGCTTTCTCGCTTCGGCTGCATTGAAGGTATTGCAGC
AGGACTGA

Structures

Toxin

• AlphaFold DB: A0A2N8AR08