Detailed information of TA system TA099944

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 320420..321090
• Replicon: chromosome
• Accession: NZ_CP027715
• Organism: Pseudomonas chlororaphis subsp. aurantiaca strain M12

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: C4K24_RS01390
• Protein ID: WP_081362979.1
• Coordinates: 320420..320848 (-)
• Length: 143 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A3G7DZE9
• Locus tag: C4K24_RS01395
• Protein ID: WP_009046453.1
• Coordinates: 320845..321090 (-)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C4K24_RS01375	315539..317797	+	2259	WP_124307425.1	TonB-dependent receptor	-
C4K24_RS01380	317844..319169	+	1326	WP_124307426.1	LLM class flavin-dependent oxidoreductase	-
C4K24_RS01385	319254..320291	+	1038	WP_124307427.1	L-glyceraldehyde 3-phosphate reductase	-
C4K24_RS01390	320420..320848	-	429	WP_081362979.1	type II toxin-antitoxin system VapC family toxin	Toxin
C4K24_RS01395	320845..321090	-	246	WP_009046453.1	plasmid stabilization protein	Antitoxin
C4K24_RS01400	321230..322069	-	840	WP_124307428.1	taurine dioxygenase	-
C4K24_RS01405	322306..323145	-	840	WP_009046455.1	taurine ABC transporter permease TauC	-
C4K24_RS01410	323142..323936	-	795	WP_102676415.1	taurine ABC transporter ATP-binding subunit	-
C4K24_RS01415	324032..325009	-	978	WP_124307429.1	taurine ABC transporter substrate-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-124)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 143 a.a.
• Molecular weight: 15853.36 Da
• Isoelectric Point: 5.4927

>T99944 WP_081362979.1 NZ_CP027715:c320848-320420 [Pseudomonas chlororaphis subsp. aurantiaca]
MIVLDTNVLSELMRPQPDTAVLLWIEAQPVEDLYISAMTMAEILHGIARLPHGKRKQTLHASAVAMFEEDFIDRIVPFGT
QAALYYAHLVSHRERSGQPINLVDAQIAAICRVHDAAIATRNTKDFTDTGLIVINPWLPLEV

Nucleotide

• Length: 429 bp

>T99944 NZ_CP027715:c320848-320420 [Pseudomonas chlororaphis subsp. aurantiaca]
ATGATAGTACTCGACACAAATGTGTTGTCAGAACTGATGCGCCCTCAACCGGATACGGCGGTATTGCTGTGGATCGAAGC
GCAACCCGTCGAGGACCTCTACATCAGCGCTATGACCATGGCCGAGATTCTCCACGGTATCGCGCGCCTGCCCCACGGCA
AGCGCAAACAGACGCTGCATGCATCGGCGGTGGCCATGTTCGAGGAAGACTTCATCGACCGGATCGTGCCCTTCGGAACC
CAAGCAGCTCTTTACTACGCGCATCTGGTTTCGCATCGCGAAAGGTCAGGCCAGCCGATCAATCTGGTGGATGCGCAAAT
TGCAGCGATATGCCGGGTCCACGACGCCGCGATCGCCACTCGTAACACCAAGGATTTCACCGACACCGGGCTGATCGTTA
TCAACCCCTGGCTGCCGCTCGAGGTCTGA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 9103.27 Da
• Isoelectric Point: 6.2314

>AT99944 WP_009046453.1 NZ_CP027715:c321090-320845 [Pseudomonas chlororaphis subsp. aurantiaca]
MASLTIRNLDDHIKERLRIEAAHNGHSMEEEARLILRRALSRTADSQGLGSRIRARFAETGGVELELPERTEPARLVDLG
E

Nucleotide

• Length: 246 bp

>AT99944 NZ_CP027715:c321090-320845 [Pseudomonas chlororaphis subsp. aurantiaca]
ATGGCCAGCCTTACGATCCGTAATCTGGACGACCACATCAAAGAACGCCTGCGCATCGAGGCCGCTCACAATGGTCATTC
CATGGAAGAAGAAGCCCGATTGATTTTGCGCCGCGCATTGAGCAGGACAGCTGACAGCCAAGGCCTGGGCAGCCGTATCA
GGGCACGCTTTGCCGAGACCGGCGGAGTAGAGCTGGAGCTGCCTGAACGTACCGAACCGGCACGCTTGGTGGACCTGGGC
GAATGA

Structures

Antitoxin

• AlphaFold DB: A0A3G7DZE9