Detailed information of TA system TA099943

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 313572..314185
• Replicon: chromosome
• Accession: NZ_CP027715
• Organism: Pseudomonas chlororaphis subsp. aurantiaca strain M12

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: C4K24_RS01360
• Protein ID: WP_096372784.1
• Coordinates: 313572..313754 (+)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A554PT90
• Locus tag: C4K24_RS01365
• Protein ID: WP_009046450.1
• Coordinates: 313784..314185 (+)
• Length: 134 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C4K24_RS01330	309338..309595	-	258	WP_124307420.1	hypothetical protein	-
C4K24_RS01335	309648..310136	-	489	WP_124307421.1	DoxX family protein	-
C4K24_RS01340	310210..311106	-	897	WP_124307422.1	SDR family oxidoreductase	-
C4K24_RS01345	311135..311644	-	510	WP_124298260.1	DUF1097 domain-containing protein	-
C4K24_RS01350	311678..312418	-	741	WP_124307423.1	SDR family oxidoreductase	-
C4K24_RS01355	312557..313447	+	891	WP_124298261.1	LysR family transcriptional regulator	-
C4K24_RS01360	313572..313754	+	183	WP_096372784.1	type II toxin-antitoxin system HicA family toxin	Toxin
C4K24_RS01365	313784..314185	+	402	WP_009046450.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
C4K24_RS01370	314285..315331	-	1047	WP_124307424.1	LacI family DNA-binding transcriptional regulator	-
C4K24_RS01375	315539..317797	+	2259	WP_124307425.1	TonB-dependent receptor	-
C4K24_RS01380	317844..319169	+	1326	WP_124307426.1	LLM class flavin-dependent oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (3-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-127)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6902.07 Da
• Isoelectric Point: 10.4588

>T99943 WP_096372784.1 NZ_CP027715:313572-313754 [Pseudomonas chlororaphis subsp. aurantiaca]
MRSRELIRMIEEDGWYLIAVKGSHHPYKHLHKPGRVTIPHPDADLPRGTIHSILKQAGLK

Nucleotide

• Length: 183 bp

>T99943 NZ_CP027715:313572-313754 [Pseudomonas chlororaphis subsp. aurantiaca]
ATGCGCAGTCGGGAACTGATCAGGATGATCGAGGAGGACGGGTGGTATCTGATCGCGGTCAAAGGCAGTCATCACCCGTA
CAAACATCTACACAAGCCGGGGCGGGTCACTATCCCTCACCCGGATGCGGATCTGCCCAGGGGCACGATCCACAGCATCT
TGAAACAGGCGGGCCTGAAATGA

Antitoxin

Protein

• Length: 134 a.a.
• Molecular weight: 14596.44 Da
• Isoelectric Point: 4.9133

>AT99943 WP_009046450.1 NZ_CP027715:313784-314185 [Pseudomonas chlororaphis subsp. aurantiaca]
MKFPVVLHKDADSGYGVIVPDVPGCFSAGHTAAQAFENVKEALSLHYEGLVADGEPLPQVHEVDVHIDNPDYAGGVWGVI
EFDITPYFGKAVRFNATLPEQLLERIDQTVKRDQRYRSRSGFLAAAALRELSA

Nucleotide

• Length: 402 bp

>AT99943 NZ_CP027715:313784-314185 [Pseudomonas chlororaphis subsp. aurantiaca]
ATGAAATTCCCGGTCGTGCTGCACAAGGATGCCGACTCCGGATATGGAGTGATCGTCCCTGATGTGCCGGGCTGTTTTTC
CGCCGGTCATACCGCTGCGCAAGCCTTCGAGAATGTGAAAGAGGCCCTGTCGCTGCATTACGAAGGCCTGGTCGCCGATG
GTGAGCCGCTGCCCCAGGTGCATGAGGTCGACGTGCATATCGACAATCCCGATTACGCCGGTGGCGTGTGGGGGGTGATC
GAGTTCGACATCACGCCTTACTTCGGCAAGGCGGTGCGTTTCAATGCGACGTTGCCGGAGCAACTGTTGGAGCGCATCGA
CCAGACGGTGAAGCGCGACCAGCGCTACCGTTCGCGCTCGGGTTTTCTCGCGGCAGCGGCATTGCGCGAGTTGTCGGCGT
AG

Structures

Antitoxin

• AlphaFold DB: A0A554PT90