Detailed information of TA system TA099941

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 5866996..5867585
• Replicon: chromosome
• Accession: NZ_CP027714
• Organism: Pseudomonas chlororaphis strain ATCC 17415

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: A0A285IHJ3
• Locus tag: C4K25_RS26460
• Protein ID: WP_062824477.1
• Coordinates: 5867286..5867585 (-)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: C4K25_RS26455
• Protein ID: WP_007924096.1
• Coordinates: 5866996..5867289 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C4K25_RS26430	5862709..5863167	+	459	WP_009045805.1	GNAT family N-acetyltransferase	-
C4K25_RS26435	5863421..5864077	+	657	WP_007924093.1	DedA family protein	-
C4K25_RS26440	5864083..5864892	+	810	WP_062824480.1	zinc-dependent peptidase	-
C4K25_RS26445	5865157..5865684	+	528	WP_114761534.1	inorganic diphosphatase	-
C4K25_RS26450	5866499..5866880	+	382	Protein_5168	DUF3077 domain-containing protein	-
C4K25_RS26455	5866996..5867289	-	294	WP_007924096.1	putative addiction module antidote protein	Antitoxin
C4K25_RS26460	5867286..5867585	-	300	WP_062824477.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C4K25_RS26465	5867757..5868206	+	450	WP_009045812.1	DUF3828 domain-containing protein	-
C4K25_RS26470	5868231..5869241	-	1011	WP_205350727.1	zinc-binding dehydrogenase	-
C4K25_RS26475	5869299..5869598	-	300	WP_007924100.1	ArsR family transcriptional regulator	-
C4K25_RS26480	5869666..5870754	-	1089	WP_062824475.1	nitrite reductase, copper-containing	-
C4K25_RS26485	5870980..5871222	+	243	WP_007924102.1	exodeoxyribonuclease VII small subunit	-
C4K25_RS26490	5871219..5872106	+	888	WP_053262814.1	(2E,6E)-farnesyl diphosphate synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (1-94)

Antitoxin

• Uncharacterized protein HI1420 (6-91)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11261.01 Da
• Isoelectric Point: 10.8652

>T99941 WP_062824477.1 NZ_CP027714:c5867585-5867286 [Pseudomonas chlororaphis]
MIEIKQTATFMEWENRLKDSRARALIAARIFRLANGLAGDVSPVGEGVSELRIHYGPGYRVYFQQRGLQLVILLCGGDKS
RQGRDIELAKQLARRWSRS

Nucleotide

• Length: 300 bp

>T99941 NZ_CP027714:c5867585-5867286 [Pseudomonas chlororaphis]
ATGATCGAGATCAAACAGACCGCGACCTTTATGGAGTGGGAAAACAGGCTCAAGGATTCCAGAGCCAGGGCGTTGATTGC
TGCGCGGATTTTTCGCCTGGCCAACGGTCTTGCCGGGGACGTGTCGCCGGTGGGCGAAGGCGTCAGTGAGCTGCGCATCC
ATTACGGGCCGGGCTACCGGGTTTACTTCCAGCAGCGCGGCTTGCAACTCGTCATTCTGTTGTGCGGCGGCGACAAGAGT
CGCCAGGGCCGCGATATCGAACTGGCCAAACAGCTGGCCCGTCGTTGGAGCCGATCATGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10430.94 Da
• Isoelectric Point: 5.6558

>AT99941 WP_007924096.1 NZ_CP027714:c5867289-5866996 [Pseudomonas chlororaphis]
MNEPLFDYDPATALRDPQAIALFMADAFETGDAGYIAKALGVVARAKGMTELAHETGLSREQLYRSFSDKGNPTLKTLLA
VMRALGLDMTARVHGAS

Nucleotide

• Length: 294 bp

>AT99941 NZ_CP027714:c5867289-5866996 [Pseudomonas chlororaphis]
ATGAACGAACCGCTTTTCGACTATGACCCCGCCACGGCCCTGCGCGACCCGCAGGCCATTGCCCTGTTCATGGCGGACGC
CTTCGAGACCGGCGATGCCGGCTACATCGCCAAAGCCCTGGGCGTGGTGGCGCGGGCCAAGGGTATGACCGAACTGGCCC
ATGAAACCGGGCTGTCTCGCGAGCAGCTGTACCGCTCATTCAGCGACAAGGGCAACCCGACCCTGAAGACCCTGCTGGCG
GTGATGCGTGCACTGGGGCTGGACATGACGGCGCGGGTGCATGGGGCGTCTTGA

Structures

Toxin

• AlphaFold DB: A0A285IHJ3