Detailed information of TA system TA099940

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/upstrm_HI1419-dnstrm_HI1420
• Location: 2301206..2301791
• Replicon: chromosome
• Accession: NZ_CP027714
• Organism: Pseudomonas chlororaphis strain ATCC 17415

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: C4K25_RS10565
• Protein ID: WP_085530375.1
• Coordinates: 2301498..2301791 (-)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: A0A285IMN7
• Locus tag: C4K25_RS10560
• Protein ID: WP_009043099.1
• Coordinates: 2301206..2301496 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C4K25_RS10550	2298721..2300103	+	1383	WP_114761927.1	efflux transporter outer membrane subunit	-
C4K25_RS10555	2300222..2300809	+	588	WP_007923530.1	hypothetical protein	-
C4K25_RS10560	2301206..2301496	-	291	WP_009043099.1	putative addiction module antidote protein	Antitoxin
C4K25_RS10565	2301498..2301791	-	294	WP_085530375.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C4K25_RS10570	2302027..2303421	-	1395	WP_114761928.1	VOC family protein	-
C4K25_RS10575	2303686..2304810	+	1125	WP_114761929.1	diguanylate cyclase	-
C4K25_RS10580	2304904..2306148	+	1245	WP_114761930.1	M20/M25/M40 family metallo-hydrolase	-
C4K25_RS10585	2306381..2306776	+	396	WP_114761931.1	carboxymuconolactone decarboxylase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (4-96)

Antitoxin

• Uncharacterized protein HI1420 (9-94)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 10736.34 Da
• Isoelectric Point: 10.0960

>T99940 WP_085530375.1 NZ_CP027714:c2301791-2301498 [Pseudomonas chlororaphis]
MDYEILQTTVFAQWHTTLRDLRARIAIARRIERASAGNLGDVKNLGGGLSEMRVNTGAGYRLYFTVRGHTLIVLLLGGDK
STQPADICQARSLAKEF

Nucleotide

• Length: 294 bp

>T99940 NZ_CP027714:c2301791-2301498 [Pseudomonas chlororaphis]
ATGGATTACGAGATCCTGCAGACGACCGTCTTTGCCCAATGGCACACCACGCTACGGGACCTGCGCGCCAGGATAGCCAT
CGCTCGGCGTATCGAGCGGGCTTCGGCGGGCAACCTGGGCGATGTGAAAAACCTCGGCGGCGGCCTCTCGGAAATGCGCG
TGAATACAGGCGCCGGCTACCGCCTCTATTTCACGGTGCGCGGCCACACCCTCATCGTCCTGCTGCTGGGTGGCGACAAG
TCCACCCAGCCTGCCGATATCTGCCAGGCCCGTTCCCTGGCCAAGGAGTTCTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10223.78 Da
• Isoelectric Point: 8.0096

>AT99940 WP_009043099.1 NZ_CP027714:c2301496-2301206 [Pseudomonas chlororaphis]
MSDTLLPFDIAALLDSDQAISEYLSQVLADGDTDELIRALGHVARARGMTQIAKATGQGRASLYKAFAPGAKPRFDTVVK
VMRALGLQLCTTVSRG

Nucleotide

• Length: 291 bp

>AT99940 NZ_CP027714:c2301496-2301206 [Pseudomonas chlororaphis]
ATGTCCGACACCCTGCTTCCCTTCGACATCGCCGCCCTGCTCGACAGCGACCAGGCGATCAGCGAATACCTGTCCCAGGT
CCTGGCCGATGGCGATACCGATGAACTGATCCGCGCCCTGGGCCATGTCGCCAGGGCGCGGGGCATGACGCAGATTGCCA
AGGCCACCGGCCAGGGCCGGGCCAGCCTCTACAAGGCTTTTGCCCCGGGGGCCAAGCCGCGTTTCGATACCGTGGTCAAG
GTCATGCGCGCGCTGGGCCTGCAGCTGTGCACCACCGTGTCTCGCGGCTGA

Structures

Antitoxin

• AlphaFold DB: A0A285IMN7