Detailed information of TA system TA099937

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 318984..319654
• Replicon: chromosome
• Accession: NZ_CP027714
• Organism: Pseudomonas chlororaphis strain ATCC 17415

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: C4K25_RS01380
• Protein ID: WP_062824000.1
• Coordinates: 318984..319412 (-)
• Length: 143 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A2N8BIH3
• Locus tag: C4K25_RS01385
• Protein ID: WP_062823999.1
• Coordinates: 319409..319654 (-)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C4K25_RS01365	314106..316364	+	2259	WP_114760181.1	TonB-dependent receptor	-
C4K25_RS01370	316411..317736	+	1326	WP_009041689.1	LLM class flavin-dependent oxidoreductase	-
C4K25_RS01375	317821..318858	+	1038	WP_114760182.1	L-glyceraldehyde 3-phosphate reductase	-
C4K25_RS01380	318984..319412	-	429	WP_062824000.1	type II toxin-antitoxin system VapC family toxin	Toxin
C4K25_RS01385	319409..319654	-	246	WP_062823999.1	hypothetical protein	Antitoxin
C4K25_RS01390	319782..320621	-	840	WP_097134161.1	taurine dioxygenase	-
C4K25_RS01395	320805..321644	-	840	WP_114760183.1	taurine ABC transporter permease TauC	-
C4K25_RS01400	321641..322435	-	795	WP_114760184.1	taurine ABC transporter ATP-binding subunit	-
C4K25_RS01405	322531..323508	-	978	WP_009041696.1	taurine ABC transporter substrate-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-127)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 143 a.a.
• Molecular weight: 15811.42 Da
• Isoelectric Point: 5.4927

>T99937 WP_062824000.1 NZ_CP027714:c319412-318984 [Pseudomonas chlororaphis]
MIVLDTNVLSELMRPQPDASVLLWIEAQPVEDLYISAMTMAEILHGIARLPHGKRKQMLHASALAMFEEDFIDRIVPFGT
QAALYYAHLISHRESLGRPISLVDAQIAAICRVHDAAIATRNAKDFSDTGLIVINPWLPLEV

Nucleotide

• Length: 429 bp

>T99937 NZ_CP027714:c319412-318984 [Pseudomonas chlororaphis]
ATGATCGTGCTCGACACTAATGTGCTGTCGGAACTGATGCGTCCCCAACCGGACGCATCGGTATTGCTGTGGATCGAAGC
GCAACCTGTCGAAGACCTCTACATCAGCGCCATGACCATGGCCGAGATTCTTCACGGCATCGCGCGCCTGCCCCACGGCA
AGCGCAAACAGATGCTCCATGCGTCGGCGCTCGCGATGTTCGAGGAAGATTTCATCGACCGCATCGTGCCCTTCGGGACC
CAGGCGGCTCTTTACTACGCCCATCTGATTTCGCACCGGGAAAGCCTGGGGCGTCCGATCAGCCTGGTGGATGCGCAAAT
CGCGGCCATATGCCGCGTCCACGACGCGGCGATCGCCACCCGTAACGCCAAGGACTTCAGCGATACAGGGTTGATCGTTA
TCAACCCCTGGCTGCCACTCGAGGTTTGA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 9129.35 Da
• Isoelectric Point: 7.3226

>AT99937 WP_062823999.1 NZ_CP027714:c319654-319409 [Pseudomonas chlororaphis]
MASLTIRNLDDHIKERLRIEAAHNGHSMEEEARLILRRALNRTTDSQGLASRIRARFAEAGGVKLELPERTEPARVVDLG
E

Nucleotide

• Length: 246 bp

>AT99937 NZ_CP027714:c319654-319409 [Pseudomonas chlororaphis]
ATGGCCAGCCTGACAATCCGAAACCTGGACGACCACATCAAAGAGCGCCTGCGCATCGAGGCCGCCCACAACGGCCACTC
CATGGAAGAAGAAGCCCGCTTGATTTTGCGCCGCGCCCTGAACAGAACAACCGACAGCCAAGGCCTGGCCAGTCGTATCC
GGGCACGCTTTGCCGAGGCCGGCGGAGTGAAGCTTGAGCTGCCCGAACGTACCGAGCCGGCACGCGTGGTGGATCTGGGC
GAATGA

Structures

Antitoxin

• AlphaFold DB: A0A2N8BIH3