Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 312150..312763 Replicon chromosome
Accession NZ_CP027714
Organism Pseudomonas chlororaphis strain ATCC 17415

Toxin (Protein)


Gene name hicA Uniprot ID A0A285EYZ4
Locus tag C4K25_RS01350 Protein ID WP_009041685.1
Coordinates 312150..312332 (+) Length 61 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A2N8BII0
Locus tag C4K25_RS01355 Protein ID WP_009041686.1
Coordinates 312362..312763 (+) Length 134 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C4K25_RS01320 307915..308172 - 258 WP_062824009.1 hypothetical protein -
C4K25_RS01325 308225..308713 - 489 WP_009041680.1 DoxX family protein -
C4K25_RS01330 308787..309683 - 897 WP_114760179.1 SDR family oxidoreductase -
C4K25_RS01335 309713..310222 - 510 WP_009041682.1 DUF1097 domain-containing protein -
C4K25_RS01340 310256..310996 - 741 WP_053259257.1 SDR family oxidoreductase -
C4K25_RS01345 311135..312025 + 891 WP_053259258.1 LysR family transcriptional regulator -
C4K25_RS01350 312150..312332 + 183 WP_009041685.1 type II toxin-antitoxin system HicA family toxin Toxin
C4K25_RS01355 312362..312763 + 402 WP_009041686.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
C4K25_RS01360 312852..313898 - 1047 WP_114760180.1 LacI family DNA-binding transcriptional regulator -
C4K25_RS01365 314106..316364 + 2259 WP_114760181.1 TonB-dependent receptor -
C4K25_RS01370 316411..317736 + 1326 WP_009041689.1 LLM class flavin-dependent oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(3-59)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(3-127)


Sequences

Toxin        


Download         Length: 61 a.a.        Molecular weight: 6933.08 Da        Isoelectric Point: 10.4588

>T99936 WP_009041685.1 NZ_CP027714:312150-312332 [Pseudomonas chlororaphis]
MRSRELIRMIEEDGWYLIAVKGSHHQYKHLHKPGRVTIPHPDADLPRGTIHSILKQAGLK

Download         Length: 183 bp

>T99936 NZ_CP027714:312150-312332 [Pseudomonas chlororaphis]
ATGCGCAGTCGGGAACTGATCAGGATGATCGAGGAGGACGGGTGGTATCTGATCGCGGTCAAAGGCAGTCATCACCAGTA
CAAGCATCTGCACAAGCCGGGGCGGGTCACTATCCCTCACCCGGATGCGGATCTGCCCAGGGGCACGATCCACAGCATCT
TGAAACAGGCGGGCCTGAAATGA

Antitoxin


Download         Length: 134 a.a.        Molecular weight: 14629.48 Da        Isoelectric Point: 4.7569

>AT99936 WP_009041686.1 NZ_CP027714:312362-312763 [Pseudomonas chlororaphis]
MKFPVVLHKDADSGYGVIVPDVPGCFSAGHTAAEAFENVKEALSLHYEGLVADGEPLPQVHEVDVHIDNPDYAGGVWGMI
EFDITPYFGKAVRFNATLPEQLLERIDQTVKRDQRYRSRSGFLAAAALRELSA

Download         Length: 402 bp

>AT99936 NZ_CP027714:312362-312763 [Pseudomonas chlororaphis]
ATGAAATTCCCGGTCGTGCTGCACAAGGATGCCGACTCCGGATATGGAGTGATCGTCCCCGATGTGCCGGGCTGTTTTTC
CGCCGGTCATACCGCAGCAGAAGCCTTCGAGAATGTGAAAGAGGCCCTGTCGCTGCATTACGAAGGCCTGGTCGCCGATG
GTGAACCGCTGCCCCAGGTGCATGAGGTCGACGTGCATATCGACAATCCCGATTACGCCGGTGGCGTGTGGGGCATGATT
GAGTTCGACATCACGCCTTACTTCGGCAAGGCGGTGCGCTTCAACGCCACCCTGCCGGAGCAATTGCTGGAACGCATCGA
CCAGACGGTGAAGCGCGACCAGCGCTACCGCTCGCGTTCGGGCTTTCTCGCCGCGGCGGCATTACGCGAGTTATCGGCGT
AG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A285EYZ4

Antitoxin

Source ID Structure
AlphaFold DB A0A2N8BII0

References