Detailed information of TA system TA099933

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/upstrm_HI1419-dnstrm_HI1420
• Location: 2376544..2377129
• Replicon: chromosome
• Accession: NZ_CP027713
• Organism: Pseudomonas chlororaphis strain TAMOak81

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: C4K26_RS11150
• Protein ID: WP_124350394.1
• Coordinates: 2376836..2377129 (-)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: A0A3G7H967
• Locus tag: C4K26_RS11145
• Protein ID: WP_062822914.1
• Coordinates: 2376544..2376834 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C4K26_RS11135	2374066..2375448	+	1383	WP_124350392.1	efflux transporter outer membrane subunit	-
C4K26_RS11140	2375568..2376155	+	588	WP_124350393.1	hypothetical protein	-
C4K26_RS11145	2376544..2376834	-	291	WP_062822914.1	putative addiction module antidote protein	Antitoxin
C4K26_RS11150	2376836..2377129	-	294	WP_124350394.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C4K26_RS11155	2377365..2378759	-	1395	WP_124350395.1	VOC family protein	-
C4K26_RS11160	2379022..2380146	+	1125	WP_124350396.1	diguanylate cyclase	-
C4K26_RS11165	2380240..2381484	+	1245	WP_124350397.1	M20/M25/M40 family metallo-hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-97)

Antitoxin

• Uncharacterized protein HI1420 (11-94)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 10800.44 Da
• Isoelectric Point: 10.0960

>T99933 WP_124350394.1 NZ_CP027713:c2377129-2376836 [Pseudomonas chlororaphis]
MDYEILQTTVFAQWHTTLRDLRARIAIARRIERASAGNLGDVKNLGGGLSEMRVNMGAGYRIYFTVRGHTFIVLLLGGDK
STQPADICQARSLAKEF

Nucleotide

• Length: 294 bp

>T99933 NZ_CP027713:c2377129-2376836 [Pseudomonas chlororaphis]
ATGGATTACGAGATCCTGCAGACGACCGTCTTTGCCCAATGGCACACCACACTACGGGACCTGCGCGCCAGGATAGCCAT
CGCTCGGCGCATCGAGCGGGCTTCGGCGGGCAACCTGGGCGATGTGAAAAACCTCGGTGGCGGTCTCTCGGAAATGCGCG
TGAACATGGGCGCCGGCTACCGGATCTATTTCACGGTGCGCGGCCACACCTTCATCGTCCTGCTGCTGGGTGGCGACAAG
TCCACCCAGCCTGCCGATATCTGCCAGGCCCGTTCCCTGGCCAAGGAGTTCTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10253.80 Da
• Isoelectric Point: 8.0096

>AT99933 WP_062822914.1 NZ_CP027713:c2376834-2376544 [Pseudomonas chlororaphis]
MSDTLLPFDIATLLDSDQAISEYLSQVLADGDTDELIRALGHVARARGMTQIAKATGQGRASLYKAFAPGAKPRFDTVVK
VMRALGLQLCTTVSRG

Nucleotide

• Length: 291 bp

>AT99933 NZ_CP027713:c2376834-2376544 [Pseudomonas chlororaphis]
ATGTCCGACACCCTGCTTCCCTTCGACATCGCCACCCTGCTCGACAGCGACCAGGCGATCAGCGAATACCTGTCCCAGGT
CCTGGCCGATGGCGATACCGATGAACTGATCCGCGCCCTGGGCCACGTCGCCAGGGCCCGGGGCATGACGCAGATTGCCA
AGGCCACCGGCCAGGGCCGGGCCAGCCTCTACAAGGCCTTTGCCCCGGGCGCCAAGCCGCGCTTCGATACCGTGGTCAAG
GTCATGCGCGCGCTGGGCCTGCAGCTGTGCACCACCGTGTCCCGAGGCTGA

Structures

Antitoxin

• AlphaFold DB: A0A3G7H967